總鐵離子含量測定試劑盒 | CheKine™ Mirco Cell Total Iron Ion Content Assay Kit Abbkine貨號KTB1114

CheKine™ Mirco Cell Total Iron Ion Content Assay Kit

產品介紹:

Iron is one of the essential trace elements of the human body, it is the main component of hemoglobin, myoglobin, cytochrome and other enzyme systems, to help oxygen transport, promote fat oxidation. Lack of iron is likely to cause anemia, metabolic disorder, and affect the body’s immune function. CheKine™ Micro Cell Total Iron Ion Content Assay Kit can be used to detect biological samples such as cells. In the kit, after cells is cracked, sodium sulfite reduces Fe3+ in the sample to produce Fe2+, Fe2+ forms a blue complex with tripyridyl triazine under acidic conditions, and has an absorption peak at 593 nm. The content of total iron ion can be calculated by measuring the absorbance of this wavelength.

鐵是人體必需的微量元素( essential trace elements)之一，是血紅素(hemoglobin)、肌紅蛋白(myoglobin)、細胞色素(cytochrome )等酵素系統的主要成分，有助於氧氣運輸，促進脂肪氧化。缺鐵容易引起貧血、代謝失調，並影響人體的免疫功能。 CheKine™微量細胞總鐵離子含量測定試劑盒可用於檢測細胞等生物樣本。試劑盒中，細胞破碎後，亞硫酸鈉還原樣本中的Fe3+生成Fe2+，Fe2+在酸性條件下與三吡啶三嗪形成藍色複合物，在593nm處有吸收峰。透過測定此波長的吸光度，即可計算出樣品中總鐵離子的含量。

樣品製備: 

樣品製備
注意：建議使用新鮮樣品，如不立即測定，樣品可在-80℃保存1個月。細胞：收集1×10 7個細胞於離心管中，用冷PBS清洗細胞，離心後棄上清；加入0.5mL萃取緩衝液，冰上裂解10分鐘，每2分鐘顛倒混勻一次，然後置於冰上待測。注意：1. 本試劑盒的萃取緩衝液不可用於蛋白含量測定，如需測定蛋白含量，需用去離子水萃取蛋白後再進行測定。如需測定樣本的蛋白質濃度，建議使用Abbkine Cat #: KTD3001 Protein Quantification Kit (BCAAssay) 測定樣本的蛋白質濃度。 2. 為避免鐵污染，所有樣品處理及轉移作業均不得使用鐵質器皿。必要時可用1%稀鹽酸浸泡設備4h。

Product name	CheKine™ Mirco Cell Total Iron Ion Content Assay Kit
SampleType	Cells
Alternative	Total Iron Ion
Kit components	• Extraction Buffer • Reagent I •Standard
Features & Benefits	The verified samples are complete in type and easy to operate．
Usage notes	• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If  don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples.
Storage instructions	Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Iron is one of the essential trace elements of the human body, it is the main component of hemoglobin, myoglobin, cytochrome and other enzyme systems, to help oxygen transport, promote fat oxidation. Lack of iron is likely to cause anemia, metabolic disorder, and affect the body’s immune function. CheKine™ Micro Cell Total Iron Ion Content Assay Kit can be used to detect biological samples such as cells. In the kit, after cells is cracked, sodium sulfite reduces Fe3+ in the sample to produce Fe2+, Fe2+ forms a blue complex with tripyridyl triazine under acidic conditions, and has an absorption peak at 593 nm. The content of total iron ion can be calculated by measuring the absorbance of this wavelength.
Alternative	Total Iron Ion