Mouse IGF-1 protein重組蛋白 | 貨號PRP1146

Mouse IGF-1 protein

胰島素樣生長因子 1 (Insulin Like Growth Factors 1, IGF-I) 是類胰島素生長因子的 7.81 kDa 成員，具有 71 個胺基酸殘基。 IGF-I主要表達於肝臟( liver)、脂肪組織(adipose tissue)、子宮頸( cervi)、子宮內膜間質細胞(endometrial stromal cells)、間質細胞，並且可以從血漿中分離。 IGF-I 介導蛋白質合成代謝和促進腦下垂體生長激素的作用。 IGF-I 也透過與 IGF-I 受體結合影響肝醣代謝、DNA 合成和葡萄糖攝取( glucose uptake)。

Product name	Mouse IGF-1 protein
Sequence	MGPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKAA with polyhistidine tag at the C-terminus.
Activity	Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is <2 ng/mL. The specific activity of recombinant mouse IGF-I is > 5 x 10⁵ IU/mg.
Protein length	The protein has a calculated MW of 8.61 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Preparation method	E. coli
Purity	>98% as determined by SDS-PAGE.
Alternative	Somatamedin C, IGF-IA
Formulation	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits	Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight	The protein has a calculated MW of 8.61 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Usage notes	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Storage instructions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution,  store at 2°C to 8°C for up to 1 week.  Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose  solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Insulin Like Growth Factors 1 (IGF-I) is a 7.81 kDa member of the Insulin-like Growth Factors with 71 amino acid residues. IGF-I is mainly expressed from liver, adipose tissue, cervi, endometrial stromal cells, leydig cells, and can be isolated from plasma. IGF-I is mediating the protein anabolic and promoting effect of pituitary growth hormone. IGF-I also affects metabolism of glycogen, DNA synthesis and glucose uptake via binding to IGF-I receptor.
Alternative	Somatamedin C, IGF-IA
Accession	P05017