EZ Cap™ Firefly Luciferase mRNA APExBIO貨號 R1018
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報告基因mRNA系列商品->>Firefly Luciferase mRNA
APExBIO Technology LLC 是一家領先的小分子抑制劑(Small Molecule Inhibitors)/活化劑(Activators)、化合物庫( Compound Libraries)、胜肽(Peptides)、檢測試劑盒(Assay Kits)、螢光標記(Fluorescent Label)、酶(Enzymes)、修飾核苷酸(Modified Nucleotides)、mRNA 合成以及各種分子生物學工具的供應商。APExBIO提供廣泛的產品線，涵蓋 20 多個不同的研究領域，例如癌症(cancer)、免疫學(immunology)、神經科學(neurosciences)、細胞凋亡(apoptosis)和表觀遺傳學(epigenetics)等。公司總部位於美國德州休士頓USA (Houston, Texas)，致力於服務全球客戶。高度重視產品品質。所有產品均遵循嚴格的生產指南，並附有分析證書、HPLC、質譜(Mass Spectrum)和HMNR以及體外驗證(in vitro validation)。 APExBIO產品已被《Nature》、《Cell》和《Science》等眾多頂級同行評審期刊引用。>>更多APExBio商品
EZ Cap™ Firefly Luciferase mRNA APExBIO貨號 R1018
EZ Cap™ Firefly Luciferase mRNA產品介紹
Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability.
EZ Cap™ 螢火蟲螢光素酶 mRNA (EZ Cap™ Firefly Luciferase mRNA) 進入細胞後會表現螢光素酶蛋白，該蛋白最初是從螢火蟲 Photinuspyralis 中提取的。此酵素催化 ATP 依賴性 D-螢光素氧化並導致在約 560 nm 波長處產生化學發光。螢火蟲螢光素酶 (Firefly Luciferase)是一種常用於基因調控和功能研究的生物發光報告基因。它適用於 mRNA 遞送 (mRNA delivery)、翻譯效率(translation efficiency)、細胞活力(cell viability)和體內成像( in vivo imaging)等檢測。目前對mRNA加帽的方法有兩種：一是共轉錄法(co-transcription method)，即在轉錄過程中添加Cap類似物。另一種是酵素法封蓋(enzymatic Capping)。轉錄後，Cap0 由痘苗病毒加帽酶 (VCE)、GTP 和 S-腺苷甲硫氨酸 (SAM) 進行加帽。然後透過 2´-O-甲基轉移酶和 SAM 將 Cap0 生成為 Cap1。 Cap1 封端也可以透過在一步過程中加入 VCE、2´-O-甲基轉移酶、GTP 和 SAM 來進行。 Cap 1結構比Cap 0結構更適合哺乳動物系統，並且具有更高的轉錄效率。添加 Poly (A) 尾巴可提高 mRNA 在體外和體內的穩定性和壽命。 Poly (A) 尾在提高翻譯起始效率方面也扮演重要角色。
Product Citation
- 1. Timothy H. Cheung, Alexander Fuchs, et al. “Acid-Responsive Polymer Additives Increase RNA Transfection from Lipid Nanoparticles.” ADVANCED FUNCTIONAL MATERIALS Volume35, Issue2 January 9, 2025 2413220
- 2. Jingjiao Li, Jie Hu, et al. “High-throughput synthesis and optimization of ionizable lipids through A3 coupling for efficient mRNA delivery.” J Nanobiotechnology. 2024 Nov 4;22(1):672. PMID: 39497197
- 3. Caitlin McMillan, Amy Druschitz, et al. “Tailoring lipid nanoparticle dimensions through manufacturing processes.” RSC Pharm. 2024 Sep 23 PMID: 39323767
- 4. Kathryn A. Whitehead, et al. “Lipid nanoparticle structure and delivery route during pregnancy dictate mRNA potency, immunogenicity, and maternal and fetal outcomes.” Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2307810121. PMID: 38437545
- 5. Peng Zhang, Xiaoqing Hu, et al. “Schlafen-11 and -9 are innate immune sensors for intracellular single-stranded DNA.” bioRxiv 28 February 2024
APExBIO提供優質的胜肽和生物分析試劑，廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域， 還提供定制的服務，包括多肽合成，抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析，高效液相色譜，質譜，和HMNR數據。
| R1001-.1 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1001-5 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1001-1 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1005-.1 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1005-5 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1005-1 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1006-.1 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1006-5 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1006-1 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1007-.1 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1007-5 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1007-1 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1008-.1 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1008-5 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1008-1 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-.1 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-5 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-1 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1010-.1 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1010-5 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1010-1 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-.1 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-5 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-1 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1012-.1 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1012-5 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1012-1 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1013-.1 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1013-5 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1013-1 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1015-.1 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1015-5 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1015-1 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1016-.1 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1016-5 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1016-1 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-.1 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-5 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-1 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1018-.1 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1018-5 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1018-1 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1020-.1 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1020-5 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1020-1 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1021-.1 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1021-5 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1021-1 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1022-.1 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1022-5 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1022-1 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1023-.1 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1023-5 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1023-1 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1024-.1 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1024-5 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1024-1 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1025-.1 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1025-5 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1025-1 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1026-.1 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1026-5 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1026-1 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |