ARCA EGFP mRNA | APExBIO貨號 R1001
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ARCA EGFP mRNA APExBIO貨號 R1001
ARCA EGFP mRNA產品介紹
EGFP (enhanced green fluorescent protein, EGFP)（增強型綠色螢光蛋白）mRNA 轉染細胞後可表達增強型綠色螢光(enhanced green fluorescence )，是一種直接檢測報告基因mRNA，可作為對照，透過各種基於螢光的檢測來研究哺乳動物細胞中的轉染和表達。您可以在 509 nm 處觀察螢光。我們的產品已經透過高效的共轉錄加帽方法用ARCA進行修飾，這意味著大多數mRNA具有Cap 0結構。它是比無帽mRNA更成熟的mRNA，並保持更穩定的狀態，從而在轉染的哺乳動物細胞中具有更高的表達效率。 ARCA 能夠僅以正確的方向插入，從而形成 mRNA，其翻譯效率是 mCAP 起始的 mRNA 的兩倍。ARCA EGFP mRNA 的升級版本現已推出，請查看 R1007 ARCA EGFP mRNA (5-moUTP) 以了解更多詳細資訊。
| Cell line | HEK293T |
| Tissue culture plate | 48-well tissue culture plate |
| The plating cell volume | 50,000 |
| Cell density before transfection | cell fusion degree 50%-60% |
| Transfection conditions | After culturing for 17 hours, transfection (1μg mRNA+2μL lipofectamine 2000) in serum- and antibiotic-free medium, 4 hours later, change to complete medium to return to normal growth environment and perform fluorescence photography after 24 hours. |
| RNA amount and transfection reagent | 1μg mRNA and 2μL lipofectamine 2000 are prepared according to the instructions |
| Transfection efficiency | above 90% transfection efficiency |
- 1. Peiying Li, et al. “Targeted mRNA Nanoparticles Ameliorate Blood–Brain Barrier Disruption Postischemic Stroke by Modulating Microglia Polarization.” ACS Nano. 2024 Jan 30;18(4):3260-3275. PMID: 38227975
- 2. Yan Liang, Jingge Zhang, et al. “Biomimetic Mineralized CRISPR/Cas RNA Nanoparticles for Efficient Tumor-Specific Multiplex Gene Editing.” ACS Nano. 2023 Aug 8;17(15):15025-15043. PMID: 37481734
- 3. Hao Chen, Lina Guo, et al. “A General and Efficient Strategy for Gene Delivery Based on Tea Polyphenols Intercalation and Self‐Polymerization.” Adv Sci (Weinh). 2023 Aug;10(24):e2302620. PMID: 37349886
- 4. Qiming Yin, Xiang Song, et al. “Incorporation of glycyrrhizic acid and polyene phosphatidylcholine in lipid nanoparticles ameliorates acute liver injury via delivering p65 siRNA.” Nanomedicine. 2022 Dec 27;48:102649. PMID: 36584740
APExBIO提供優質的胜肽和生物分析試劑，廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域， 還提供定制的服務，包括多肽合成，抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析，高效液相色譜，質譜，和HMNR數據。
| R1001-.1 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1001-5 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1001-1 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1005-.1 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1005-5 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1005-1 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1006-.1 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1006-5 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1006-1 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1007-.1 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1007-5 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1007-1 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1008-.1 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1008-5 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1008-1 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-.1 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-5 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-1 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1010-.1 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1010-5 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1010-1 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-.1 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-5 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-1 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1012-.1 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1012-5 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1012-1 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1013-.1 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1013-5 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1013-1 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1015-.1 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1015-5 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1015-1 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1016-.1 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1016-5 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1016-1 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-.1 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-5 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-1 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1018-.1 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1018-5 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1018-1 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1020-.1 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1020-5 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1020-1 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1021-.1 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1021-5 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1021-1 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1022-.1 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1022-5 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1022-1 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1023-.1 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1023-5 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1023-1 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1024-.1 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1024-5 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1024-1 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1025-.1 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1025-5 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1025-1 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1026-.1 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1026-5 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1026-1 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |