草醯乙酸測定試劑盒| Oxaloacetate Assay Kit貨號Abnova™KA3793
| 代理廠牌： | |
| 原廠連結： | |
| 相關下載： |
草醯乙酸測定試劑盒| Oxaloacetate Assay Kit貨號Abnova™KA3793
Use of 20 µL sample. Linear detection range 2 µg/mL to 50 µg/mL collagen in 96-well plate assay.
100 assays
草醯乙酸 (OXALOACETATE, OAA) 是檸檬酸循環( citric acid cycle )中的中間體，參與糖質新生作用。 OAA是經由蘋果酸(malate)的氧化、天門冬胺酸的脫醯胺作用或CO2與丙酮酸(pyruvate)或磷酸烯醇丙酮酸(phosphoenolpyruvate)的縮合形成的。草醯乙酸測定試劑盒( Oxaloacetate Assay Kit)提供了一個簡單、直接且自動化的程序來測量草醯乙酸(oxaloacetate )濃度。 OAA 轉化為丙酮酸( pyruvate)，然後丙酮酸( pyruvate)被氧化，染料轉化為有色螢光形式。氧化染料在 570 nm 處的顏色強度或 λex/em = 530/585 nm 處的螢光強度與樣本中草醯乙酸(oxaloacetate )濃度成正比。
應用：
✓ 應用直接測定：血漿、血清、組織和培養基中的草醯乙酸。
特點：
✓靈敏且準確：線性檢測範圍：比色測定為 7 至 400 µM 草醯乙酸，螢光測定為 1 至 40 µM。
Product Description
Oxaloacetate Assay Kit is a quantitative colorimetric/fluorimetric determination of oxaloacetate at OD570, FL530/585 nm.
Suitable Sample
Culture Medium, Plasma, Serum, Tissue
Sample Volume
20 uL
Detection Method
Colorimetric, Fluorimetric
Assay Type
Quantitative
Calibration Range
Colorimetric: 7 to 400 uM, Fluorimetric: 1 to 40 uM
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store the kit at -20°C.