Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag), HPLC-verified貨號10004-H08H

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Her2/ERBB2 重組蛋白（ECD，His 標籤） | SinoBiological貨號10004-H08H

Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

產品介紹:人表皮生長因子受體 2 (Human epidermal growth factor receptor 2, HER2)，也稱為 ErbB2、NEU 和 CD340，是一種 I 型膜糖蛋白(type I membrane glycoprotein )，屬於表皮生長因子 (epidermal growth factor, EGF) 受體家族。 HER2蛋白由於缺乏自身的配體結合域(ligand binding domain)而不能結合生長因子，並且是組成性的自抑制( autoinhibited constitutively)。然而，HER2 與其他配體結合的 EGF 受體家族成員形成異二聚體，因此穩定配體結合( ligand binding)併增強激酶介導( kinase-mediated activation)的下游分子激活。 HER2 在發育(development)、細胞增殖(cell proliferation)和分化(differentiation)中發揮著關鍵作用。據報導，HER2基因與多種癌症的惡性腫瘤(malignancy)和不良預後相關，包括乳腺癌(breast)、前列腺癌(prostate)、卵巢癌(ovarian)、肺癌( lung cancers )等。

SinoBiological提供重組蛋白系列商品；其中Her2/ERBB2 重組蛋白（ECD，His 標籤），蛋白質建構(rotein Construction)為人類ErbB2 (NP_004439.2)胞外結構域(Met 1-Thr 652)的DNA序列與C端的多組胺酸標籤融合，Accession#NP_004439.2。表達的宿主HEK293細胞。以 SDS-PAGE 測定純度> 90 %。以 SEC-HPLC 測定，> 90%。以 LAL 方法測定，內毒素< 1.0 EU/μg 蛋白質。分子量重組人ErbB2含有641個胺基酸，計算分子量為71 kDa。由於糖基化，rh ErbB2 在還原條件下在 SDS-PAGE 中遷移為大約 100-110 kDa 的蛋白質。

Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED₅₀ for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag)(Cat:10004-H08H) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab)(Cat:68046-H002), the EC₅₀ is 10-60 ng/mL.

Captured anti-Her2 IgG1 antibody on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.270 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Captured Trastuzumab (68046-H002) on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.727 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Loaded Anti-Erbb2 Antibody (Trastuzumab) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.547 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Activity

1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag)(Cat:10004-H08H) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab)(Cat:68046-H002), the EC₅₀ is 10-60 ng/mL.
2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED₅₀ for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
3.Captured anti-Her2 IgG1 antibody on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.270 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
4.Captured Trastuzumab (68046-H002) on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.727 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
5.Loaded Anti-Erbb2 Antibody (Pertuzumab) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.382 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
6.Loaded Anti-Erbb2 Antibody (Trastuzumab) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.547 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.

Sino Biological Inc. 為全球生醫製藥研究領域中最大的cDNA、重組蛋白質和抗體製造供應商之一，近年來更致力於生產研發臨床用GMP等級重組蛋白質(GMP Recombinant Protein)。Sino Biological在流感病毒等製病性病毒研發快速領先全球。Sino Biological Inc團隊擁有多年重組蛋白研發經驗。多年來，我們的團隊已經生成了超過6000種重組蛋白(recombinant proteins)，涵蓋了免疫檢查點(immune checkpoints)、抗體藥物靶點(antibody drug targets)、CAR-T細胞治療靶點( CAR-T cell therapy targets)、Fc受體(Fc receptors)、流感病毒蛋白( influenza viral proteins)和細胞因子(cytokines)等最熱門的研究領域。重組蛋白具有高純度和經過驗證的生物活性，可以支持您從生物製藥靶點發現、蛋白質結構和功能分析、細胞治療和重組酶表徵等方面的科學研究。

Catalog #	Species	Molecule	Application
10084-R015	Human	CD274	ELISA, IHC-P
10004-R511	Human	ERBB2	ELISA, ELISA(Cap), FCM, lCC/IF, IF
10565-R102	Human	CD226	ELISA
10231-R301-P	Human	LDLR	FCM
10259-R110	Human	FCGR2B	WB, IHC-P, IP
10773-R023	Human	ASGR1	ELISA
10004-R002	Human	ERBB2	ELISA, IHC-P, FCM, ICC/IF, IF
10001-R021	Human	EGFR	WB, ELISA, ELISA(Det), ICC/IF, IF, IP
10694-R028	Human	EpCAM	WB, ELISA, ELISA(Det), IHC-P, FCM, ICC/IF, IF, IP
13909-R226	Human	F8	ELISA
10005-R017	Human	PVRL2	ELISA
10377-RD67	Human	PDCD1	ELISA, IHC-P
10594-RM03	Human	PCSK9	ELISA
10186-R125	Human	BSG	WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP
10136-R111-P	Human	FLT1	FCM
10390-R024	Human	HAVCR2	ELISA, ELISA(Cap)
10390-R108	Human	HAVCR2	ELISA
11066-R105	Human	VEGFA	ELISA
11215-R107	Human	LAMP1	WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP
11235-R019	Human	REG3A	ELISA, IHC-P
10973-R111	Human	vWF	ELISA, ELISA(Det), IHC-P
11050-R412	Human	NGF	ELISA
11279-R021	Human	CTNNB1	ELISA, IHC-P, ICC/IF, IF, IP
13908-R002	Human	ASGR2	ELISA, IHC-P, FCM, ICC/IF, IF
10008-R064	Human	VEGFA	ELlSA, ELISA(Det)
100087-R013	Human	FGF2	HC-P
10014-R101	Human	FGF2	ELISA
100393-R024-F	Human	GPC3	FCM
100393-R024-P	Human	GPC3	FCM
10084-R001	Human	CD274	ELISA
10231-R301-F	Human	LDLR	FCM
10289-R102	Human	LGALS3	HC-P
10400-R113	Human	CD4	EIISA, IHC-P
10417-R006	Human	TNFRSF1B	ELISA
10980-R081	Human	CD8A	HC-P
11051-Rn208	Human	HAVCR1	ELISA
12078-R001	Human	GLA	W8, ELISA, IP
10007-R104	Human	CSF3	ELISA
10073-R001	Human	CD14	IHC-P
10160-R005	Human	CFD	ELISA
10259-R112	Human	FCGR2B	ELISA.ELISA(Det)
10327-RM04	Human	MMP9	ELISA
10995-R018	Human	SerpinG1	WB, ELISA, ELISA(Det), IP
11031-R039	Human	CD8B	HC-P
11192-R179	Human	CD68	HC-P
11996-R351	Human	KIT	ELISA, IHC-P
12163-R005	Human	VSIG4	ELISA, ELISA(Det)
13908-R002-P	Human	ASGR2	FCM
100128-R001	Human	CD79A	IHC-P
100129-R101	Human	KRT14	WB, IHC-P, IP
100254-R211	Human	VIM	IHC-P

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