BCA蛋白定量試劑盒(1-2000 µg/ml) | Super-Rapid Protein Quantification Kit (BCA Assay) KTD3010-EN
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特點
BCA蛋白定量試劑盒 | 貨號KTD3010-EN 1-2000 µg/ml
The kit is suitable for the detection of protein concentrations in the range of 1-2,000 µg/mL, and the sample volume to be tested is 1-20 µL.
BCA法蛋白定量試劑盒是常用的蛋白質濃度檢測方法之一。超快速蛋白定量試劑盒（BCA Assay）與傳統BCA蛋白定量方法類似，但採用了不同於BCA的全新特異性螯合劑，從而實現快速、穩定、靈敏的蛋白濃度測定。此方法的原理是蛋白質在鹼性條件下將銅離子（Cu2+）還原為亞銅離子（Cu+），生成的Cu+與螯合物形成橙黃色水溶性複合物，在480 nm處有強吸收峰，吸光度值與樣本中蛋白質的含量成正比，根據吸光度值即可測定蛋白質濃度。此試劑盒中的螯合劑可以靈敏、特異地與 Cu+ 結合，只需在室溫下孵育 5 分鐘即可完成顯色反應。
This product has been cited in 2 publications
Abbkine 蛋白定量試劑盒產品特點:
- 節省時間: 5分鐘室溫顯色反應。比傳統的Lowry method方法更容易快四倍。
- 靈敏度: 檢測低至1 µg/mL
- 吸光度準確：採用比色法，在480 nm處吸光度最佳
- 良好的線性度: 線性工作範圍從 1到2000 μg/ml.
- 待測樣品體積：1-20μL
| Product name | Super-Rapid Protein Quantification Kit (BCA Assay) |
| Applications notes | The kit is suitable for the detection of protein concentrations in the range of 1-2,000 µg/mL, and the sample volume to be tested is 1-20 µL. |
| Detection method | Colorimetric |
| Kit components | • Reagent A • Reagent B • BSA Standard |
| Features & Benefits | (1) Fast and time-saving: 5 min room temperature color development reaction; (2) Wide measurement range: wide linear working range, 1-2000 μg/mL protein concentration; (3) High sensitivity: detection of protein concentration as low as 1 μg/mL; (4) Accurate absorbance: the colorimetric method is used to have the best absorbance at 480 nm; (5) Sample volume to be measured: 1-20 μL. |
| Calibration range | 1-2,000 µg/mL |
| Limit of detection | 1 µg/mL |
| Usage notes | 1. The protein concentration determined by method is not affected by chemical substances in most samples, and can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween20, 60, 80 in the sample. However, the method is affected by chelating agents and slightly higher concentrations of reducing agents. It is necessary to ensure that EDTA is less than 10 mM, no dithiothreitol (DTT) and β-mercaptoethanol. 2. It is recommended to make a standard curve for each measurement. Because the color of the method will continue to deepen with the extension of time, and the color reaction will be accelerated due to the increase of temperature. 3. If the sample diluent or lysate itself has a high background, please try the Protein Quantification Kit (Bradford Assay) (Cat #:KTD3002). |
| Storage instructions | Store according to the recommended storage conditions of each component, stable for 12 months. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. |
| Background | The BCA method protein quantification kit is one of the commonly used detection methods for protein concentration. Super-Rapid Protein Quantification Kit (BCA Assay) is similar to the traditional BCA protein quantitation method, but employs a completely new and specific chelator different from BCA, resulting in a rapid, stable, and sensitive determination of protein concentration. The principle of the method is that the protein reduces copper ion (Cu2+) to cuprous ion (Cu+ ) in basic conditions, and the generated Cu+ forms an orange yellow water-soluble complex with chelates and has a strong absorption peak at 480 nm, the absorbance value is proportional to the content of protein in the sample, and the protein concentration can be determined according to the absorbance value. The chelators in this kit can sensitively and specifically bind to Cu+ and complete the color reaction with only 5 min incubation at room temperature. |
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