人類腫瘤壞死因子α（TNF-α）ELISA 試劑盒 |Human tumor necrosis factor α (TNF-α) ELISA Kit貨號CK-bio-13850
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
人類腫瘤壞死因子α（TNF-α）ELISA 試劑盒 |Human tumor necrosis factor α (TNF-α) ELISA Kit貨號CK-bio-13850
Human tumor necrosis factor α (TNF-α) ELISA Kit
偵測範圍:20pg/ml -800pg/ml , 最低靈敏度10.0pg/ml
Double speed Elisa 試劑盒在 90 -120分鐘內即可快速獲得實驗結果；競爭法(competitive method)2-3小時。 保持著卓越的偵測專一性與靈敏度。即用型標準品含有6瓶濃度，不需自行配製。每瓶 0.5ml (濃度依不同產品有所不同) 。
產品描述：該試劑盒用於人類腫瘤壞死因子α（TNF-α）水平，基於雙重原理(sandwich technology)抗體夾心技術酶聯免疫吸附試驗（enzyme linked immunosorbent assay , ELISA）。將標準品(Standard)和Sample 加入到預先包被有目標抗體的孔中，然後加入HRP-Conjugate 試劑形成免疫複合物，孵育，通過孵育和洗滌，去除未結合的酶，然後加入底物A和B，溶液會轉藍色，最後在酸的作用下變成黃色。顏色深度或光是與人類腫瘤壞死因子α（TNF-α）濃度呈正相關。偵測範圍:20pg/ml -800pg/ml, 最低靈敏度10.0pg/ml。可應用於以下樣品來源:血清(Serum)、血漿(plasma)、尿液(urine)、唾液(saliva)、組織勻漿(tissue homogenates)、細胞裂解物(cell lysates)、細胞培養物上清液(cell culture supernates)和其他生物體(biological fluids)液。
人類腫瘤壞死因子α（TNF-α）ELISA 試劑盒
| Name | Human tumor necrosis factor α (TNF-α) ELISA Kit |
| Cat No. | CK-bio-13850 |
| Specification | 48T/96T/5*96T/10*96T |
| Detection range | 20pg/ml -800pg/ml |
| Detection limit | 10.0pg/ml |
| Test principle | Quantitative |
| Test method | Sandwich |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Sample type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
For the quantitative determination of Human tumor necrosis factor α (TNF-α) concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates and other biological fluids.
Note: 提供己配製好的標準品6瓶 Standard (A → F) concentration was followed by: 0pmol/L ,20pmol/L ,40pmol/L ,80pmol/L ,160pmol/L ,320pmol/L
樣品準備要求 (Specimen Requirements)-血清/血漿/細胞/尿液 (實際操作以原廠操作手冊為主)
5天內使用的樣品可在2-8℃保存，否則樣品必須在-20℃（≤1個月）或-80℃（≤2個月）保存，以免生物活性喪失，避免污染。
1.血清 (Serum)：
讓(Serum)在室溫下凝結10-20分鐘。離心（2000-3000 RPM）20 分鐘。仔細收集上清液(supernatants)。儲存過程中出現沉澱時，應重新離心。
2.血漿(Blood plasma)：
根據樣本採集要求，使用EDTA或檸檬酸鈉(sodium citrate)抗凝(anti coagulation)。添加 EDTA 或檸檬酸鈉並混合 10-20 分鐘。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
3.尿液(Urine)：
用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。採集胸腹水、腦脊液時，請按上述程序進行。
4.細胞培養上清液(Cell culture supernatant)：
檢查分泌成分(secrete components)時用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。檢查細胞內成分時，用PBS（PH 7.2-7.4）將細胞懸液稀釋至細胞濃度約100萬/ml。通過反覆凍融循環損壞細胞，釋放內部成分。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
5.組織樣品(Tissue sample)：
切取樣品並稱重。添加一定量的PBS（PH 7.4）。立即用液氮冷凍備用。解凍樣品並保存在2-8℃。加入一定量的PBS（PH 7.4），然後用手或均質器將樣品徹底均質。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。等分並保留一份用於檢查，其餘的冷凍以備後用。
Coon-koon台灣代理商-BRAND/AGENCY/代理品牌-Coon-koon
http://www.coonkoonbio.com/
Shanghai Coon Koon Biotech Co.,Ltd是一家專業從事生物製品研發、生產和經營的綜合性生物公司！ 產品遠銷多個國家Coon Koon Bio主要做ELISA試劑盒、生化試劑、抗體等上萬種科研產品！涉及分子生物學、細胞生物學、免疫學等各個領域！堅持“以質量為前提，以客戶滿意為宗旨”；確立“以客戶需求為導向，以市場需求為導向，不斷開發優質新產品，提供客戶滿意。
| CK-bio-18527 | Bovine Hyaluronidase(HAase)ELISA Kit |
| CK-bio-18528 | Bovine Hydroxyproline(HYP)ELISA Kit |
| CK-bio-18529 | Bovine Immunoglobulin A,IgA ELISA Kit |
| CK-bio-18530 | Bovine Immunoglobulin G,IgG ELISA Kit |
| CK-bio-18531 | Bovine Immunoglobulin G2a(IgG2A)ELISA Kit |
| CK-bio-18532 | Bovine Immunoglobulin M,IgM ELISA Kit |
| CK-bio-18533 | Bovine Infectious Bronchitis Antibody(IBV)ELISA Kit |
| CK-bio-18534 | Bovine Infectious Bursal Disease Antibody(IBD)ELISA Kit |
| CK-bio-18535 | Bovine Infectious Laryngotracheitis Antibody(ILT)ELISA Kit |
| CK-bio-18536 | Bovine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit |
| CK-bio-18537 | Bovine Insulin,INS ELISA Kit |
| CK-bio-18538 | Bovine Insulin-like growth factors 1,IGF-1 ELISA Kit |
| CK-bio-18539 | Bovine Integrin alpha-L(ITGAL)ELISA Kit |
| CK-bio-18540 | Bovine Integrin αM,ITG/CD11b ELISA Kit |
| CK-bio-18541 | Bovine Intercellular Adhesion Molecule 1(ICAM-1)ELISA Kit |
| CK-bio-18542 | Bovine Interferon tau(IFN-tau)ELISA Kit |
| CK-bio-18543 | Bovine Interferon α,IFN-α ELISA Kit |
| CK-bio-18544 | Bovine Interferon β,IFN-β/IFNB ELISA Kit |
| CK-bio-18545 | Bovine Interferon γ ,IFN-γ ELISA Kit |
| CK-bio-18546 | Bovine Interleukin 1,IL-1 ELISA Kit |
| CK-bio-18547 | Bovine Interleukin 10(IL-10)ELISA Kit |
| CK-bio-18548 | Bovine Interleukin 12,IL-12 ELISA Kit |
| CK-bio-18549 | Bovine Interleukin 17,IL-17 ELISA Kit |
| CK-bio-18550 | Bovine Interleukin 17A,IL-17A ELISA Kit |
| CK-bio-18551 | Bovine Interleukin 18(IL-18)ELISA Kit |
| CK-bio-18552 | Bovine Interleukin 1α,IL-1α ELISA Kit |
| CK-bio-18553 | Bovine Interleukin 1β,IL-1β ELISA Kit |
| CK-bio-18554 | Bovine Interleukin 2,IL-2 ELISA kit |
| CK-bio-18555 | Bovine Interleukin 21,IL-21 ELISA Kit |
| CK-bio-18556 | Bovine Interleukin 4,IL-4 ELISA Kit |
| CK-bio-18557 | Bovine Interleukin 6,IL-6 ELISA Kit |
| CK-bio-18558 | Bovine Interleukin 8,IL-8 ELISA Kit |
| CK-bio-18559 | Bovine intestinal fatty acid binding protein,iFABP ELISA Kit |
| CK-bio-18560 | Bovine Inward rectifier potassium channel 2,KCNJ2 ELISA Kit |
| CK-bio-18561 | Bovine Islet amyloid polypeptide,IAPP ELISA Kit |
| CK-bio-18562 | Bovine Isocitrate dehydrogenase [NADP] cytoplasmic(IDH1) ELISA Kit |
| CK-bio-18563 | Bovine Isocitrate dehydrogenase [NADP] mitochondrial(IDH2) ELISA Kit |
| CK-bio-18564 | Bovine Japanese Encephalitis IgG(JEV IgG) ELISA Kit |
| CK-bio-18565 | Bovine Junctional adhesion molecule A(F11R)ELISA Kit |
| CK-bio-18566 | Bovine Kappa-casein(CSN3)ELISA Kit |
| CK-bio-18567 | Bovine Kininogen-1,KNG1/HMWK ELISA Kit |
| CK-bio-18568 | Bovine latent transforming growth factor beta binding protein 2,LTBP2 ELISA Kit |
| CK-bio-18569 | Bovine Lecithin Cholesterol Acyltransferase(LCAT)ELISA Kit |
| CK-bio-18570 | Bovine Legumain(LGMN)ELISA Kit |
| CK-bio-18571 | Bovine Leptin,LEP ELISA Kit |
| CK-bio-18572 | Bovine Leptospira IgG(Lep IgG)ELISA Kit |
| CK-bio-18574 | Bovine leukocyte common antigen,LCA/CD45 ELISA Kit |
| CK-bio-18575 | Bovine Leukotriene B4 receptor 1,LTB4R ELISA Kit |
| CK-bio-18576 | Bovine L-Lactate Dehydrogenase(L-LDH)ELISA Kit |
| CK-bio-18577 | Bovine Luteinizing Hormone,LH ELISA Kit |
| CK-bio-18578 | Bovine Lymphatic vessel endothelial hyaluronic acid receptor 1(LYVE-1) ELISA Kit |
| CK-bio-18579 | Bovine Lymphotoxin-alpha,LTA ELISA Kit |
| CK-bio-18580 | Bovine Lys-63-specific deubiquitinase BRCC36,BRCC3/BRCC36 ELISA Kit |
| CK-bio-18581 | Bovine lysophosphatidic acid(LPA)ELISA Kit |
| CK-bio-18582 | Bovine Lysozyme(LZM)ELISA Kit |
| CK-bio-18583 | Bovine Lysyl oxidase-like protein 1,LOXL1 ELISA kit |
| CK-bio-18584 | Bovine Metallothionein-1A(MT1A) ELISA kit |
| CK-bio-18585 | Bovine α-Melanocyte Stimulating Hormone(α-MSH) ELISA Kit |
| CK-bio-18586 | Bovine Melatonin,MT/MLT ELISA Kit |
| CK-bio-18587 | Bovine menbrane IgA(mIgA)ELISA Kit |
| CK-bio-18588 | Bovine Mesencephalic astrocyte-derived neurotrophic factor,MANF ELISA Kit |
| CK-bio-18589 | Bovine Metallothionein-2(MT-2) ELISA Kit |
| CK-bio-18590 | Bovine Monocyte Chemotactic Protein 1(MCP-1)ELISA Kit |
| CK-bio-18591 | Bovine Myo-inositol oxygenase,MIOX ELISA Kit |
| CK-bio-18592 | Bovine Myostatin,MSTN ELISA Kit |
| CK-bio-18593 | Bovine N-acetyl-β-D-glucosaminidase,NAG ELISA Kit |
| CK-bio-18594 | Bovine Inhibin alpha chain(INHA) ELISA kit |
| CK-bio-18595 | Bovine Nesfatin-1 ELISA Kit |
| CK-bio-18596 | Bovine NF-kappa-B inhibitor alpha(NFKBIA)ELISA Kit |
| CK-bio-18597 | Bovine Nitric Oxide(NO)ELISA Kit |
| CK-bio-18598 | Bovine neuropeptide Y (NPY) ELISA kit |
| CK-bio-18599 | Bovine Non-histone chromosomal protein HMG-14,HMG14 ELISA kit |
