輔酶 Ⅱ NADP(H) 測定試劑盒 | CheKine™ Micro Coenzyme Ⅱ NADP(H) Assay Kit貨號KTB1010
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輔酶 Ⅱ NADP(H) 測定試劑盒 | CheKine™ Micro Coenzyme Ⅱ NADP(H) Assay Kit貨號KTB1010
偵測範圍0.5 µM-10 µM
產品描述：
煙醯胺腺嘌呤二核苷酸磷酸 (Nicotinamide adenine dinucleotide phosphate, NADP) 是一種酵素輔助因子(enzymatic cofactor)，參與許多氧化還原反應(redox reactions )，在還原 ( reduced , NADPH) 和氧化 (oxidized , NADP) 形式之間循環。 NADP 也參與生物合成反應，例如脂質( lipid )和核酸合成(nucleic acid synthesis )，其中它充當還原劑( reducing agent)。磷酸戊糖途徑 (pentose phosphate pathway , PPP) 的氧化分支( oxidative branch)是動物細胞中產生的 NADPH 的主要來源。人們一直對監測其濃度水平感興趣。 NADP+ /NADPH 的定量測定可應用於細胞或組織的能量轉化和氧化還原狀態的研究。輔酶 Ⅱ NADP(H) 檢測試劑盒為靈敏檢測 NADP 和 NADPH 及其在各種組織和亞細胞器(subcellular organelles) 中的比例提供了便捷的工具。此試劑盒基於酵素循環反應（不識別 NAD+/NADH），其中形成的NADPH reduces a formazan (MTT) 試劑。在 OD565 nm 處測量的還原產物顏色的強度與樣品中的 NADP+ /NADPH 濃度成正比。
| Product name | CheKine™ Micro Coenzyme Ⅱ NADP(H) Assay Kit |
| Applications notes | Abbkine CheKine™ Micro Coenzyme Ⅱ NADP(H) Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NAD+/NADH), in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. |
| Kit components | • Assay Buffer • Glucose (1 M) • WST-8 Solution • Enhancer Solution • NADP Cycling Enzyme Mix • NADPH standard (10 mM) • NADP Extraction Buffer • NADPH Extraction Buffer |
| Features & Benefits | • Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts. • Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay. |
| Calibration range | 0.5 µM-10 µM |
| Usage notes | • Addition of Working Reagent should be quick and mixing should be brief but thorough. • The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%). |
| Storage instructions | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues. |
Image & description
Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).
引用文獻
Apelin, Omentin-1, and Vaspin in patients with essential hypertension: association of adipokines with trace elements, inflammatory cytokines, and oxidative damage markers(KTB2130-1.1)
Author：Serinkan Cinemre FB, Cinemre H, Bahtiyar N, Kahyaoğlu B, Ağaç MT, Shundo H, Sevinç L, Aydemir B Publication name：Ir J Med Sci IF：1.1