膽紅素測定試劑盒 | Bilirubin Assay Kit ABNOVA貨號KA1614

| 代理廠牌: | |
| 原廠連結: | |
| 相關下載: |
膽紅素測定試劑盒 | Bilirubin Assay Kit ABNOVA貨號KA1614
最低檢測範圍0.16 mg/dL

膽紅素(BILIRUBIN)是紅血球(red blood cells)死亡時形成的血紅蛋白( hemoglobin)的降解產物之一。存在膽紅素不溶性非結合形式( insoluble unconjugated form)(也稱為間接膽紅素),或可溶性葡萄醣醛酸結合形式(soluble glucuronide conjugated form)膽紅素(也是直接膽紅素)。結合膽紅素( Conjugated bilirubin)進入肝臟的膽小管(bile canaliculi ),然後進入膽囊(gall bladder)。當受到進食刺激時,膽汁(包括結合膽紅素)被排泄到小腸,在那裡膽紅素(bilirubin )轉化為尿膽素原(urobilinogen)。膽紅素(bilirubin 一個關鍵的診斷指標。當以下情況時會導致膽紅素(bilirubin 水平升高過多的血紅蛋白被分解或膽紅素(bilirubin) 的去除功能無法正常發揮。積累體內膽紅素(bilirubin )過多會引起黃疸。簡單且自動化的膽紅素定量測定程序在以下領域得到廣泛應用:研究和藥物發現。測定原理(Principle of the Assay) 膽紅素檢測試劑盒設計用於測量 96 孔或比色皿形式的血液樣本中的膽紅素(bilirubin )。改進的 Jendrassik-Grof 方法利用膽紅素(bilirubin )與重氮化磺胺酸(diazotized sulfanilic acid)的反應,其中紅色形成有色產物。在 510-550 nm 處測量的顏色強度可以準確測量樣本中的膽紅素(bilirubin )水平。使用苯甲酸咖啡因(caffeine benzoate)從膽紅素中分離膽紅素來評估總膽紅素未結合膽紅素蛋白複合物。


✓ 直接測定:血清中總膽紅素和直接膽紅素
✓ 藥理學:藥物對膽紅素代謝的影響。
✓ 靈敏、準確。 96 孔板測定中膽紅素的檢測限為 0.16 mg/dL。
✓ 簡單且高通量。該過程包括添加單一工作試劑和孵育10分鐘。可以在 96 孔板中輕鬆實現自動化高通量檢測,進行數千次檢測
- Product Description:
- Bilirubin Assay Kit is a quantitative colorimetric bilirubin determination at 530 nm.
- Sample Volume:
- 50 uL
- Detection Method:
- Colorimetric
- Assay Type:
- Quantitative
- Limit of Detection:
- 0.16 mg/dL
- Regulation Status:
- For research use only (RUO)
- Quality Control Testing:
- Standard Curve

The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Storage Instruction:
- Store the kit at 4°C.
Publication Reference
1.Comparison of Purgative Manna Drop and Phototherapy with Phototherapy Treatment of Neonatal Jaundice: A Randomized Double-Blind Clinical Trial.
Monsef A, Eghbalian F, Rahimi N.Osong Public Health Res Perspect. 2019 Jun;10(3):152-157. doi: 10.24171/j.phrp.2019.10.3.06.
2.Glutamine inhibits CCl4 induced liver fibrosis in mice and TGF-β1 mediated epithelial-mesenchymal transition in mouse hepatocytes.
Nirajan S, Lokendra C, Han MK, Lee SO, Kim CY, Jeong YJ.Food Chem Toxicol. 2016 Apr 29. [Epub ahead of print]
3.Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease.
Ambade A, Satishchandran A, Gyongyosi B, Lowe P, Szabo G.World J Gastroenterol. 2016 Apr 28; 22(16): 4091-4108
4.Decreasing Mitochondrial Fission Prevents Cholestatic Liver Injury.
Yu T, Wang L, Lee HJ Biol Chem. 2014 Oct 23. pii: jbc.M114.588616.