Resazurin Cell Viability Assay Kit (alamarBlue™) | Biotium 貨號#30025-1 (25ml)
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Resazurin Cell Viability Assay Kit (alamarBlue™) | Biotium 貨號#30025-1 (25ml)
A sensitive, safe and cost-effective high-throughput homogeneous assay of cell viability by fluorescence or absorbance measurement.
刃天青細胞活力測定(Resazurin Cell Viability Assay)提供了一種簡單、快速、可靠、靈敏、安全且經濟高效的細胞活力測量方法。此適用螢光和可見光偵測。與其他基於刃天青(resazurin-based )的細胞增殖試劑盒（例如 alamarBlue®、PrestoBlue® 或 CellTiter-Blue®）相比，該測定具有優異的性能。單步、同質、高通量細胞定量用於基於螢光或吸光度的測量靈敏，只需 80 個細胞即可獲得可重複的結果。廣泛用於培養的哺乳動物細胞(cultured mammalian cells)、細菌(bacteria)、原生動物(protozoa)、酵母(yeast)和真菌(fungi)；不需要細胞裂解(Cell lysis )，允許長期動力學監測無毒、無放射性、無致癌性、處置簡單即用型解決方案。刃天青(Resazurin)通過響應細胞生長引起的生長培養基的化學還原，從非螢光染料轉變為高紅色螢光染料試鹵靈來檢測細胞代謝。持續的細胞生長維持還原環境，而抑制生長則維持氧化環境。刃天青(Resazurin)原為深藍色、無螢光性，然而經過粒線體中的NADH 去氫酶作用後，刃天青(Resazurin)還原成為粉紅色，透過偵測螢光 (Ex/Em: 530-560 nm/590 nm) 或吸光值 (570 nm) 吸光度進行檢測。螢光或比色信號與樣品中活細胞的數量成正比。添加底物後 1-24 小時即可獲得結果。刃天青即用型溶液(Resazurin solution)以 25 mL（2500 次測定）或 100 mL（10,000 次測定）規格提供。
刃天青(Resazurin) Cell Viability Assay優點
廣泛用於培養的哺乳動物細胞(cultured mammalian cells)、細菌(bacteria)、原生動物(protozoa)、酵母(yeast)和真菌(fungi)
1. 允許長期動力學監測 (無毒、無放射性、無致癌性、無細胞溶解) 直接加入培養液中，同步及時進行藥物對細胞毒性的動力學分析
2.操作簡單，直接培養於medium中30分鐘反應
3. 不須DMSO回溶步驟，故不易pipetting error，較MTT assay準確
4. 靈敏度較佳， 80 個細胞即可獲得可重複的結果
5.適用螢光或是可見光機器
查看我們完整的細胞活力和細胞凋亡檢測選擇 (Cell Viability & Apoptosis Assays)
Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This assay has excellent performance compared to other resazurin-based cell proliferation kits such as alamarBlue®, PrestoBlue®, or CellTiter-Blue®.
細胞生長創造了一個減少的環境，同時抑制生長氧化環境。響應生長培養基的化學還原刃天青（紫色且無螢光）是細胞生長的結果還原形成紅色螢光染料試鹵靈 (1-3)。刃天青的還原可以通過測量螢光或吸光度來監測。螢光和測定產生的比色信號與活體數量成正比樣品中的細胞。刃天青測定的靈敏度與 [3H]胸苷測定檢測細胞增殖（1）。根據細胞類型，刃天青測定可以可用於檢測少至 40 個細胞，並具有可重複且靈敏的信號。
alamarBlue 是 Trek Biosystems 的註冊商標。 CellTiter-Blue 是 Promega 公司的註冊商標。 PrestoBlue 是 Thermo Fisher Scientific 的註冊商標。alamarBlue is a registered trademark of Trek Biosystems. CellTiter-Blue is a registered trademark of Promega Corporation. PrestoBlue is a registered trademark of Thermo Fisher Scientific.
| Apoptosis/viability marker | Metabolic activity |
|---|---|
| For live or fixed cells | For live/intact cells |
| Detection method/readout | Microplate reader (absorbance), Microplate reader (fluorescence) |
| Assay type/options | Endpoint assay, High-throughput assay, Homogeneous assay, Real-time imaging |
| Colors | Red |
| Storage Conditions | Store at 2 to 8 °C |
| Excitation/Emission | 530-560/590 nm, 570 nm (for absorbance-based assay) |
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Biotium 提供範圍廣泛的螢光探針用於研究凋亡細胞(Apotosis)
The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.