乳糖測定試劑盒 | Lactose Assay Kit ABNOVA貨號KA1672
| 代理廠牌： | |
| 原廠連結： | |
| 相關下載： |
乳糖測定試劑盒 | Lactose Assay Kit ABNOVA貨號KA1672
線性檢測範圍0.2 to 10 mM
乳糖（Lactose, C12H22O11），也稱為乳糖(milk sugar)，是由β-D-半乳糖(β-D-galactose )和α/β-D-葡萄糖(β-D-galactose )組成的二糖通過β1-4糖苷鍵(β1-4 glycosidic linkage)。乳糖(Lactose)是主要的糖，佔牛奶的 2-8%。簡單、直接、乳糖測定的高通量測定有著廣泛的應用。 乳糖測定試劑盒(Lactose Assay Kit )用於定量測定乳糖(lactose)。該試劑盒使用特定的酶偶聯反應，其中乳糖被裂解，所得半乳糖( galactose)形成有色產物。 570 nm 處的顏色強度或 530 nm/585 nm 處的螢光強度與樣品中的乳糖濃度成正比。使用少至 20 μL 的樣品。 96 孔板的線性檢測範圍：17 至 2000 µM 乳糖比色測定和 6 至 100 µM 螢光測定。
✓ 牛奶和其他生物樣品中乳糖的測定。
✓ 藥物發現/藥理學：藥物對乳糖代謝的影響。
✓ 食品和飲料：食品和飲料產品中的乳糖。
Specification
- Specification
- Product Description:
- Lactose Assay Kit is for quantitative determination of lactose. The Kit uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570 nm or fluorescence intensity at 530 nm/585 nm is directly proportional to the lactose concentration in the sample.
- Suitable Sample:
- Food and Beverages Products, Milk and other Biological Samples.
- Sample Volume:
- 20 uL
- Detection Method:
- Colorimetric, Fluorometric
- Assay Type:
- Quantitative
- Calibration Range:
- Colorimetric Assay: 17 to 2000 uM, Fluorimetric Assay: 6 to 100 uM
- Regulation Status:
- For research use only (RUO)
- Quality Control Testing:
- Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Storage Instruction:
- Store the kit at -20°C.
Publication Reference
1.Acute and chronic effects of cortisol on milk yield, the expression of key receptors, and apoptosis of mammary epithelial cells in Saanen goats.
G F Bomfim, G K F Merighe, S A de Oliveira, J A Negrao.J Dairy Sci. 2022 Jan;105(1):818-830. doi: 10.3168/jds.2021-20364. Epub 2021 Oct 28.
2.Dietary Zinc-Amino Acid Complex Does Not Affect Markers of Mammary Epithelial Integrity or Heat Stability of Milk in Mid-Lactating Cows.
Shaffer JE, Mamedova LK, DeFrain JM, Pandalaneni K, Amamcharla JK, Takiya CS, Bradford BJ.Biol Trace Elem Res. 2018 Oct 31. doi: 10.1007/s12011-018-1556-y. [Epub ahead of print]