Minute™ 突觸體分離試劑盒|Synaptosome Isolation Kit Invent貨號SY-052
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快速蛋白質提取試劑盒系列-Rapid Protein Extraction Kits
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。避免 RIPA 不溶性蛋白質的問題基於旋轉柱的方法獲得更完整的蛋白質譜。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。
Minute™ 突觸體分離試劑盒 | Invent貨號SY-052
Minute™ Synaptosome Isolation Kit (50 preps)
手冊和協議 | 材料安全數據表 (MSDS) | 參考文獻 (2)
從神經元組織/培養細胞中分離突觸體的能力對於了解神經系統疾病的機制至關重要。突觸體中含有直徑為80-200 nm的突觸小泡，在信號傳遞中發揮著重要作用。傳統上，突觸體是通過密度超速離心分離的。該過程相對繁瑣且耗時。通常需要大量的起始材料。分鐘TM 突觸體分離試劑盒提供了一種從新鮮/冷凍神經元組織/培養細胞中分離突觸體的簡單快速的方法。該方案可在 1 小時內完成，無需使用 Dounce 均質器和超速離心。所需起始材料的量（10-50 mg 組織）僅是傳統方法所需量的一小部分。使用的緩衝液不含去污劑，突觸體相關蛋白以天然形式分離。
The ability to isolate synaptosomes from neuronal tissues/cultured cells is essential for understanding the mechanisms of neurological diseases. Contained in synaptosomes are synaptic vesicles with diameters of 80-200 nm that play an important role in signal transmission. Traditionally, synaptosomes are isolated by density ultracentrifugation. The procedure is relatively tedious and time-consuming. A larger amount of starting material is usually required. MinuteTM synaptosome isolation kit provides a simple and rapid method for isolating synaptosomes from fresh/frozen neuronal tissues/cultured cells. The protocol can be completed in less than 1 h without using Dounce homogenizer and ultracentrifugation. The amount of starting material required (10-50 mg tissue) is only a fraction of that required by the traditional method. The buffers used are detergent-free and synaptosome associated proteins are isolated in native form.
圖。1。從新鮮小鼠皮質中分離突觸體。
A，麗春紅 S 染色印跡。B，蛋白質印跡。泳道 1. 總組織裂解液；
泳道 2. 胞漿部分；泳道 3. 突觸體部分。突觸體
標記抗體（GluN2B 和 Glu A2/A3/A4）來自 Cell Signaling。
Fig.1. Isolation of Synaptosomes from Fresh Mouse Cortex.
A, Ponceau S-stained blot. B, Western blot. Lane 1. Total tissue lysate;
Lane 2. Cytosolic fraction; Lane 3. Synaptosome fraction. Synaptosome
marker antibodies (GluN2B and Glu A2/A3/A4) are from Cell Signaling.
適用於粘液植物的 Minute™ 葉綠體分離試劑Kit includes:
| Buffer A | 25 ml |
| Buffer B | 3 ml |
| Buffer C | 25 ml |
| Plastic Rods | 2 |
| Filter Cartridges | 50 |
| 2 ml Collection Tubes | 50 |
基於旋轉柱(SPIN-COLUMN-BASED )的蛋白質提取/細胞分級(CELL FRACTIONATION)
Invent Biotechnologies 成立於 2010 年，因革新蛋白質提取(protein extraction)和細胞分離(cell fractionation)而享譽全球。我們開發了基於旋轉柱(spin column-based)的創新技術，用於蛋白質研究( protein research)，可簡化和增強樣品製備。這會帶來更順暢的工作流程和始終可靠的結果，從而帶來卓越的數據質量。選擇 Minute™ 試劑盒進行蛋白質提取(protein extraction)和細胞分級分離(cell fractionation)的研究人員可以相信，他們的樣品準確地代表了所研究的細胞或組織的自然特徵。這些套件只需要一台台式離心機，最大限度地減少實驗變量並確保高質量的材料以獲得更可靠的數據。Minute™ 方法的有效性得到了越來越多 journals的支持 (Nature, Science, and Cell.)。
The Minute™ 的優點：
• 超快（快至 1 分鐘）
• 樣本量小
• 最小化交叉污染
• 內源性基線不變
• 所有活體和組織類型
The Minute™ 應用：
• 蛋白質運輸/易位
• 蛋白質-蛋白質相互作用
• 蛋白質修飾
• 信號轉導
• sc/snRNA-seq
Invent Minute™ 徹底改變蛋白質樣品製備和細胞分級分離
自 2010 年成立以來，我們的首要目標是利用我們基於旋轉柱的創新技術徹底改變全球蛋白質提取(protein extraction)和細胞分離(cell fractionation)市場。我們的目標是讓 Minute™ 試劑盒成為標準實驗室程序，取代所有基於溶液的樣品製備方法。通過利用這些套件，研究人員只需一台台式離心機即可獲得一致的結果並獲得卓越的數據質量。
改變蛋白質提取(Transforming protein extraction)
Minute™ 總蛋白提取試劑盒採用簡單的旋轉柱( simple spin column )，可有效消除細胞或組織裂解過程(lysis)中的非系統性蛋白質損失(non-systematic protein loss)。這代表著相對於傳統的基於解決方案的方法，例如 RIPA 緩衝液(RIPA buffer)和超聲處理(sonication)的顯著進步，這些方法以其可變性和無法提供全面的蛋白質譜而聞名。用於總蛋白提取的 Minute™ 試劑盒與來自各種生物體和組織類型的小樣本量兼容，為研究人員提供了未改變的內源蛋白基線( endogenous proteins)，從而實現更精確的定量和分析。
Invent Biotech’s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.
改進細胞分級分離(Revamping cell fractionation)
由於缺乏簡單的實驗方案，傳統的細胞分級分離方法長期以來一直受到用戶偏差和結果不一致的影響。 Minute™ 細胞分離試劑盒徹底改變了這一過程，使研究人員能夠在 90 分鐘內獲得高純度的細胞器群，並且使用最少的洗滌劑。通過採用簡單的旋轉柱，這些試劑盒無需均質化(homogenization)、密度梯度分離(density gradient separation)和超速離心等傳統技術( ultra-centrifugation)，從而減少了實驗變量的數量。這可以防止細胞器損傷( organelle damage)並產生更一致和生理相關的結果。憑藉多種 Minute™ 試劑盒可滿足不同的細胞分級(cell fractionation)分離要求，我們正在推動蛋白質組研究的轉型。
支持所有研究學科(Supporting all research disciplines)
Minute™ 試劑盒旨在無縫集成到現有的蛋白質提取和細胞分級分離工作流程中，提供快速且一致的性能，確保可靠的結果。然而，不要只相信我們的話——我們的產品已在科學文獻中被廣泛引用，這表明研究界對簡化且可靠的樣品製備方法的需求不斷增長。立即切換到 Minute™，體驗我們技術的優勢，無論您的研究領域如何。
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| SC-003 | Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests) |
| SN-006 | Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) |
| SM-005 | Minute TM Plasma Membrane Protein Isolation Kit (50 Tests) |
| SM-005-p | Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests) |
| MP-007 | Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests) |
| MM-038 | Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests) |
| SD-008/SN-009 | Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) |
| SN-010 | Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) |
| CP-011 | Minute TM Chloroplast Isolation Kit (50 Tests) |
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| SC-012 | Minute TM Single Cell Isolation Kit (50 Tests) |
| NE-013 | Minute TM Nuclear Envelope Protein Extraction Kit (50 Tests) |
| HP-014 | Minute TM Histon/DNA Binding Protein Extraction Kit (50 Tests) |
| YT-015 | Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) |
| YD-016 | Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) |
| YM-017 | Minute™ Yeast Mitochondria Enrichment Kit (50 tests) |
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| HD-021 | MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests) |
| AT-022 | MinuteTM Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Tests) |
| AF-023 | MinuteTM Adipose Tissue Fractionation Kit (20 Tests) |
| NI-024 | MinuteTM Detergent-Free Nuclei Isolation Kit (20 Tests) |
| BN-020 | MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps) |
| SN-047 | MinuteTM Single Nucleus Isolation Kit for Tissues/Cells (20 preps) |
| AN-029 | MinuteTM Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests) |
| FE-025 | MinuteTM Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests) |
| MS-026 | MinuteTM Total Protein Extraction Kit for Mass Spectrometry (50 Tests) |
| EI-027 | MinuteTM Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG) |
| ED-028 | MinuteTM Endosome Isolation and Cell Fractionation Kit (20 Tests) |
| SE-030 | MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG) |
| CS-031 | MinuteTM Cell Suspension Isolation from Fresh/Frozen Tissues (50 tests) |
| NT-032 | MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 tests) |
| NM-033 | MinuteTM Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests) |
| LY-034 | MinuteTM Lysosome Isolation Kit (20 tests) |
| ER-035 | MinuteTM High Fidelity ER Isolation Kit for Liver Tissue (20 tests) |
| ER-036 | MinuteTM ER Enrichment Kit (20 tests) |
| GO-037 | MinuteTM Golgi Apparatus Enrichment Kit (20 tests) |
| LR-039 | MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) |
| LR-042 | MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) |
| PT-040 | MinuteTM Cytosolic Proteasome Enrichment Kit (20 tests) |
| PN-041 | MinuteTM Nuclear Proteasome Enrichment Kit (20 tests) |
| PF-045 | MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) |
| SV-043 | MinuteTM Native Protein Precipitation Kit from Saliva (20 tests) |
| ML-044 | MinuteTM Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) |
| RD-046 | MinuteTM Rubisco Depletion Kit (20 tests) |
| PR-048 | MinuteTM Plant ER Enrichment Kit (20 tests) |
| PG-049 | MinuteTM Plant Golgi Apparatus Enrichment Kit (20 tests) |
| PE-050 | MinuteTM Plant Endosome Enrichment Kit (20 tests) |
| PL-051 | MinuteTM Plant Lipid Raft Isolation Kit (20 tests) |
| SY-052 | MinuteTM Synaptosome Isolation Kit (50 preps) NEW! |
| SA-01-SK | MinuteTM Total Protein Extraction Kit for Skin Tissue (50 tests) |
| SA-02-BT | MinuteTM Total Protein Extraction Kit for Bone Tissue (50 tests) |
| SA-03-BV | MinuteTM Total Protein Extraction Kit for Blood Vessels (50 tests) |
| SA-04-TD | MinuteTM Total Protein Extraction Kit for Tendons (50 tests) |
| SA-05-IM | MinuteTM Total Protein Extraction Kit for Intestines and Mesentery (50 tests) |
| SA-06-MS | MinuteTM Total Protein Extraction Kit for Muscles (50 tests) |
| SA-07-IS | MinuteTM Total Protein Extraction Kit for Insects (50 tests) |
| WA-002 | Minute™ Ab Enhancer (30 ml) |
| WA-003 | Minute™ WB Stripping Solution (250 ml) |
| WA-004 | MinuteTM Ponceau S Stain (250 ml) |
| WA-005 | Minute TM Peroxidase Suppressor (30 ml) |
| WA-006 | Minute TM High Efficiency Protein Precipitation kit |
| WA-007 | MinuteTM RBC Lysis Buffer (250 ml) |
| WA-008 | MinuteTM WB Blocking Solution (250 ml) |
| WA-009 | MinuteTM Denaturing Protein Solubilization Reagent (10 ml) |
| WA-010 | MinuteTM Non-Denatured Protein Solubilization Reagent (10 ml) |
| WA-011 | MinuteTM Protein Solubilization Reagent for MS (2 ml) |
| WA-012 | MinuteTM SDS-Remover (10 ml) |
| WA-013 | MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml) |
| WA-014 | MinuteTM Anti-Clumping Nuclei Storage Buffer NEW! |