EZ Cap™ Reagent AG | Co-transcription capping reagent APExBIO貨號B8176

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共轉錄加帽試劑( Co-transcription capping reagent ) –EZ Cap™ Reagent AG

APExBIO提供優質的胜肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。
EZ Cap™ Reagent AG Co-transcription capping reagent APExBIO貨號B8176
EZ Cap Reagent AG 是一種共轉錄加帽試劑(co-transcription capping reagent ),用於體外轉錄 5′ 加帽 mRNA 以產生 Cap 1 結構m7G(5′)ppp(5′)(2’OMeA)pG。文獻顯示 Cap AG 可提供高達 98% 的封蓋效率(capping efficiency )。EZ Cap AG 需要 AG 啟動器(AG initiator)。 EZ Cap AG 通常提供 >90% 的封端材料(capped material ),生成天然存在的 Cap 1 結構(Cap 1 structure)。EZ Cap 與野生型 T7 聚合酶( wild-type T7 polymerase)兼容。建議使用 5 mM EZ Cap 和 rNTP。模板(Template )設計是任何轉錄的一個組成部分。 EZ Cap 試劑 AG 可與特定起始序列 5′ AG 3′ 一起使用。最終加蓋效率(capping efficiency)取決於 EZ Cap 試劑、DNA 模板(DNA template)和最終 mRNA 序列。 RNA 長度和鹼基組成(base composition)引起的二級結構會影響最終的加帽效率( capping efficiency)。



- 1. Yu Zhang, Xiaodong Xi, et al. “Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice.” Mol Ther Nucleic Acids. 2022 Aug 13;29:657-671. PMID: 36090760
- 2. Binfan Chen, Yuchen Chen, et al. “A Single Dose of Anti-HBsAg Antibody-Encoding mRNA-LNPs Suppressed HBsAg Expression: a Potential Cure of Chronic Hepatitis B Virus Infection.” mBio. 2022 Aug 30;13(4):e0161222. PMID: 35862767
- 3. Shudan Tan, Xue Hu, et al. “Preclinical evaluation of RQ3013, a broad-spectrum mRNA vaccine against SARS-CoV-2 variants.” biorxiv.
- 4. Jing Lu, Guoliang Lu, et al. “A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice.” Cell Res. 2020;1-4. PMID: 32801356
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1211.62 (free acid) |
| Formula | C32H40N15Na3O24P4 (free acid) |
| Synonyms | EZ Cap™ Reagent AG for co-transcriptional capping of mRNA,m7G(5′)ppp(5′)(2’OMeA)pG |
| SDF | Download SDF |
| Canonical SMILES | O=C1NC(N)=NC2=C1[N+](C)=CN2[C@@H]3[C@@H](O)[C@@H](O)[C@H](COP([O-])(OP([O-])(OP([O-])(OC[C@H]4O[C@@H](N5C=NC6=C5N=CN=C6N)[C@H](OC)[C@@H]4OP([O-])(OC[C@H]7O[C@@H](N8C=NC9=C8N=C(N)NC9=O)[C@H](O)[C@@H]7O)=O)=O)=O)=O)O3.[Na+].[Na+].[Na+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |

APExBIO提供優質的胜肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。
