SuperKine™ Lipo3.0高效轉染試劑| Lipo3.0 Efficient Transfection Reagent Abbkine貨號BMU111-EN

new cationic liposome transfection reagent

SuperKine™ Lipo3.0高效轉染試劑是一種高效的新型陽離子脂質體轉染試劑(new cationic liposome transfection reagent )，適用於多種動物細胞質體DNA(plasmid DNA)和siRNA轉染，對大多數動物細胞具有較高的轉染效率(transfection efficiency )。SuperKine™ Lipo3.0 Efficient Transfection Reagent is a highly efficient new cationic liposome transfection reagent suitable for plasmid DNA and siRNA transfection in various animal cells, with high transfection efficiency for most animal cells.

Specification

Product name	SuperKine™ Lipo3.0 Efficient Transfection Reagent
Applications notes	SuperKine™ Lipo3.0 Efficient Transfection Reagent is a highly efficient new cationic liposome transfection reagent suitable for plasmid DNA and siRNA transfection in various animal cells, with high transfection efficiency for most animal cells.

Product Properties

Formulation	Liquid solution
Kit components	Transfection Reagent
Features & Benefits	• High transfection efficiency: The staining efficiency of conventional animal cells, such as HEK293, HeLa, etc. is as high as 90-95%. •Strong compatibility: it is not affected by serum and the medium no need to be changed before and after transfection.
Usage notes	Thaw all the components at room temperature before opening
Storage instructions	Stored at 4℃ for 12 months, avoid freezing
Shipping	Blue ice
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

A.Transfection of DNA

1.Cells were grown in suitable well plates or dish esone day before transfection to a density of 70-80% at the time of transfection.

2.Replace with fresh culture medium before transfection.

3.Preparation of Transfectionreagent/DNAcomplex:

(1)DNA-Opti-MEM: Add 25μL Opti-MEM or other serum-free, antibiotic-free cell medium to a 1.5mL sterile EP tube, then add 0.5 μg DNA to the tube (Asanexample,0.5mg DNA was transfected ineach well of 24-well plate,refertoTable1), gently mix with a pipette. (2)Transfectionreagent-Opti-MEM:Add 25μL Opti-MEM or other serum-free, antibiotic-free cell medium to another 1.5mL terile EP tube, then add1.5μL Transfect ion reagent to the tube (1-5μL/μgDNA,3μL/μgDNAisrecommended), gentlymix with a pipette. (3)Add the Transfection reagent-Opti-MEMtoDNA-Opti-MEM, gently mix with apipette, afters tandingat room temperature for 10-15min,it can be used for transfection.

4.Transfection:Dropthe Transfection reagent/DNAcomplex into the culture medium and gently shake the culture plate or dish to even lydisperse the Transfection reagent/DNA.

B. Transfection of siRNA
1. Cells were grown in suitable well plates or dishes one day before transfection to a density of 70-80% at the time of transfection.

2. Replace with fresh culture medium before transfection.

3. Preparation of siRNA: Prepare siRNA into a 20 μM storage solution using RNase-free H2O.

4. Preparation of Transfection reagent/siRNA complex:

(1) siRNA-Opti-MEM: Add 25 μL Opti-MEM or other serum-free, antibiotic-free cell medium to a 1.5 mL sterile RNase-freeEPtube, then add 1 μL 20 μM siRNA storage solutionto the tube (As an example, 20 pmol siRNA was transfected ineachwell of24-well plate, refer to Table 2), gently mix with a pipette.

(2) Transfection reagent-Opti-MEM: Add 25 μL Opti-MEM or other serum-free, antibiotic-free cell mediumto another 1.5mLsterileRNase-free EP tube, then add 1.2 μL Transfection reagent to the tube (0.4-1 μL/10 pmol siRNA, 0.6 μL/10 pmol siRNAisrecommended), gently mix with a pipette.

(3) Add the Transfection reagent-Opti-MEM to siRNA-Opti-MEM, gently mix with a pipette, after standing at roomtemperaturefor10-15 min, it can be used for transfection.

5. Transfection: Drop the Transfection reagent/siRNA complex into the culture medium and gently shake the culture plateor dish to evenly disperse the Transfection reagent/siRNA.

6. Continue to culture for 24-72 h and test with