BlasTaq™ 探針一步法 RT-qPCR | ABMGOOD貨號G596

BlasTaq™ Probe One-Step RT-qPCR

BlasTaq™ Probe One-Step RT-qPCR 使用 RT-qPCR 酶混合物和 BlasTaq™ Probe 2X qPCR MasterMix 在單個反應管中，進行高靈敏度(highly sensitive )和特異性逆轉錄( specific reverse transcription)以及基於 TaqMan 探針的實時 qPCR 擴增，提供了便利。專有的 RT-qPCR 酶混合物包含下一代 BlasTaq™ DNA 聚合酶以及穩定劑和增強劑，可在“一步”中優化兩個反應。這為最終用戶提供了 RNA 模板和引物選擇的靈活性、高效且易於使用的設置，以及傳統“兩步”順序 RT-qPCR 的可靠替代方案。 MasterMix 適用於 SNP 基因分型分析(SNP genotyping assays)、基因表達分析(gene expression analysis)、微陣列驗證(microarray validation)和高通量篩選應用(high throughput screening applications)。 ROX 參考染料單獨提供，使其與大多數 qPCR 儀器普遍兼容。

產品特點：

• Simple, one-step RT-qPCR reaction
• Flexibility with RNA templates and primer selection
• ROX reference dye provided separately
• Universally compatible with most qPCR instruments
• Earn 2X the rewards points

Product Component	Quantity
BlasTaq™ Probe 2X qPCR MasterMix	1.25 ml
RT-qPCR Enzyme Mix	100 rxn (40 μl)
ROX Reference Dye	15 μl
Nuclease-Free H2O	1.0 ml

 

BlasTaq™ Probe One-Step RT-qPCR comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE	MACHINE
High ROX Machines	ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series Biometra TOptical Fluidigm BioMark™ Wafergene SmartChip System TianLong TL998 System
Low ROX Machines	ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7 BioGene InSyte™ Illumina Eco Analytikjena qTower Series Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines	BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™ Roche LightCycler® (480, 1536, Nano) MJ Research Opticon™, Opticon™ 2, Chromo® 4 Eppendorf™ Realplex 4 BioGene SynChron™ Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat) Cepheid SmartCyler, GeneXpert Enigma™ ML Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS Qiagen Rotor-Gene™ (Q, 6000) Takara Dice™ Thermo Scientific PikoReal DNA-Technology DT96, DTlite, DT-322 Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680) Bioneer Exicycler™