唾液皮質醇檢測ELISA試劑盒| Human Cortisol (COR) ELISA Kit貨號CK-bio-11044
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唾液皮質醇（Cortisol, COR）檢測ELISA試劑盒（競爭法） 貨號CK-bio-11044
Human Cortisol(COR) ELISA Kit (competitive method)
偵測範圍: 100pg/mL -3200pg/mL , 最低靈敏度17.3pg/mL
皮質醇(Cortisol)（唾液）酶聯免疫吸附測定（ELISA）試劑盒是一種體外測定唾液中皮質醇(Cortisol)的定量測定。皮質醇(Cortisol)是一種從激素ACTH(adrenocorticotropic hormone)引起的從腎上腺皮質釋放的類固醇激素。它可以增加血壓和血糖水平，並抑制免疫系統。皮質醇(Cortisol)通過特定的細胞內受體起作用，並且對許多生理系統有影響，包括免疫系統(immune function)，葡萄糖對抗調節(glucose counter regulation)，血管緊張度(vascular tone)，底物利用和骨代謝(bone metabolism)。它作為游離形式存在於血液中，或與皮質類固醇結合球蛋白（CBG）結合。血液中只有1-10％的皮質醇以未結合/游離的形式存在，這一部分代表了引起生物反應的皮質醇(Cortisol)的活性池，並且能夠擴散到靶組織和唾液中。研究一致報導唾液和血清皮質醇水平之間高度相關，表明唾液皮質醇可以可靠地估計血清皮質醇水平。 CoonKoon的皮質醇（Saliva）ELISA試劑盒是一種競爭性酶免疫測定試劑盒(competitive method)。將 Standard 和 Sample 添加到預先包被目的抗體的孔中，然後加入鏈黴親和素 HRP 形成免疫複合物，孵育，通過孵育和洗滌，去除未結合的酶，然後加入底物A和B，則溶液在酸的作用下會變成藍色，最後變成黃色。顏色深淺與檢測唾液皮質醇（Cortisol, COR）的濃度呈負相關。100pg/mL -3200pg/mL , 最低靈敏度17.3pg/mL。唾液樣本：在 2-8°C 下以 1000×g 離心樣本 20 分鐘。收集上清液(clear supernatant )並立即進行測定。注意事項： 1.5天內使用的樣品可在2-8℃保存，否則樣品必須在-20℃（≤1個月）或-80℃（≤2個月）保存，以免失去生物活性，避免污染及反覆冷凍。
即用型標準品含有6瓶濃度，不需自行配製。每瓶 0.5ml (濃度依不同產品有所不同)
Double speed Elisa 試劑盒在 90 -120分鐘內即可快速獲得實驗結果； 保持著卓越的偵測專一性與靈敏度
For the quantitative determination of Human Cortisol(COR) concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates,body fluid and other biological fluids.
產品描述：本試劑盒採用競爭法(competitive method)檢測樣品中檢測唾液皮質醇（Cortisol, COR）的含量。將 Standard 和 Sample 添加到預先包被目的抗體的孔中，然後加入鏈黴親和素 HRP 形成免疫複合物，孵育，通過孵育和洗滌，去除未結合的酶，然後加入底物A和B，則溶液在酸的作用下會變成藍色，最後變成黃色。顏色深淺與檢測唾液皮質醇（Cortisol, COR）的濃度呈負相關。100pg/mL -3200pg/mL , 最低靈敏度17.3pg/mL。可應用於以下樣品來源:血清(Serum)、血漿(plasma)、尿液(urine)、唾液(saliva)、組織勻漿(tissue homogenates)、細胞裂解物(cell lysates)、細胞培養物上清液(cell culture supernates)和其他生物體(biological fluids)液。
唾液皮質醇（Cortisol, COR） ELISA 試劑盒
| Name | General Melatonin(MT) ELISA Kit |
| Cat No. | CK-bio-20626 |
| Specification | 48T/96T/5*96T/10*96T |
| Detection range | 100pg/mL -3200pg/mL |
| Detection limit | 17.3pg/mL |
| Test principle | Quantitative |
| Test method | competitive assay |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Sample type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
Sample collection and storages
1.Serum: Allow the serum to clot for 10-20 minutes at room temperature. Centrifuge (at 2000-3000 RPM) for 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
2.Blood plasma: In accordance with the requirements of sample collection, EDTA or sodium citrate should be used as anti coagulation. Add EDTA or sodium citrate and mix them for 10-20 minutes. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
3.Urine: Collect by sterile tube. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
4.Cell culture supernatant: Collect by sterile tubes when examining secrete components. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (PH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
5.Tissue sample: Incise sample and weigh up. Add a certain amount of PBS (PH 7.4). Freeze with liquid nitrogen immediately for later use. Thaw the sample and keep it at 2-8℃. Add a certain amount of PBS (PH 7.4) and then homogenize the sample thoroughly by hand or homogenizer. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. Aliquot and keep one for examination and freeze the others for later use.
6.saliva sample: Centrifuge samples for 20 minutes at 1000×g at 2-8°C. Collect supernatant and carry out the assay immediately.
Note: 1.Samples to be used within 5 days may be stored at 2-8℃, otherwise samples must be stored at -20℃ (≤1month) or -80℃ (≤2 months) to avoid loss of bioactivity and avoid contamination.
2.Sample hemolysis will influence the result, so the samples should be centrifuged adequately and no hemolysis or granule was allowed.
3.When performing the assay, bring samples to room temperature.
Samples containing NaN3 can’t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).
4.After collecting the sample, extraction should be immediately carried out in accordance with related documents. After extraction, experiment should be conducted immediately as well. Otherwise, keep the sample at -20℃. Avoid repeated freeze-thaw cycles.
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Shanghai Coon Koon Biotech Co.,Ltd是一家專業從事生物製品研發、生產和經營的綜合性生物公司！ 產品遠銷多個國家Coon Koon Bio主要做ELISA試劑盒、生化試劑、抗體等上萬種科研產品！涉及分子生物學、細胞生物學、免疫學等各個領域！堅持“以質量為前提，以客戶滿意為宗旨”；確立“以客戶需求為導向，以市場需求為導向，不斷開發優質新產品，提供客戶滿意。
