Goat F(ab’)2 Anti-Mouse Kappa-HRP抗體 | 貨號1052-05

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Goat F(ab’)2 Anti-Mouse Kappa-HRP抗體 | 貨號1052-05 |規格1ml
Goat F(ab’)2 Anti-Mouse Kappa-HRP antibody for use in ELISA, immunohistochemistry, and ELISpot assays.

產品介紹: 山羊 F(ab’)2 抗小鼠 Kappa-HRP 抗體,用於 ELISA、免疫組織化學和 ELISpot 測定。對mouse κ light chains具有專一性,來源為Pepsin digest of Goat Anti-Mouse Kappa (SB Cat. No. 1050)。交叉吸附(Cross Adsorption ) Mouse λ light chains; may react with κ light chains from other species。緩衝液為50% Glycerol/50% Phosphate buffered saline, pH 7.4。
| Isotype | Goat F(ab’)2 IgG |
| Isotype Control | 0110-05 |
| Specificity | Reacts with mouse κ light chains |
| Source | Pepsin digest of Goat Anti-Mouse Kappa (SB Cat. No. 1050) |
| Cross Adsorption | Mouse λ light chains; may react with κ light chains from other species |
| Conjugate | HRP (Horseradish Peroxidase) |
| Buffer Formulation | 50% Glycerol/50% Phosphate buffered saline, pH 7.4 |
| Clonality | Polyclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Quality tested applications for relevant formats include – ELISA 1 Flow CytometryOther referenced applications for relevant formats include – Immunohistochemistry-Paraffin Sections 8 ELISpot 1 Stimulation 2-7 |
| RRID Number | AB_2794387 |
| Gene ID | 243469 (Mouse) |
| Gene ID Symbol | Igk (Mouse) |
| Gene ID Aliases | kappa |
| UniProt ID | P01837 (Mouse) |
| UniProt Name | IGKC_MOUSE (Mouse) |
文獻資料
1. Velez M, Kane M, Liu S, Gauld SB, Cambier JC, Torres RM, et al. Ig allotypic inclusion does not prevent B cell development or response. J Immunol. 2007;179:1049-57. (ELISA, ELISPOT)
2. Donahue AC, Fruman DA. Distinct signaling mechanisms activate the target of rapamycin in response to different B-cell stimuli. Eur J Immunol. 2007;37:2923-36. (Stim)
3. Hobeika E, Levit-Zerdoun E, Anastasopoulou V, Pohlmeyer R, Altmeier S, Alsadeq A, et al. CD19 and BAFF-R can signal to promote B-cell survival in the absence of Syk. EMBO J. 2015;34:925-39. (Stim)
細胞免疫專家極力推薦SouthernBiotech高品質二級抗體
美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全,包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質(FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)各種螢光標誌、另具酵素(HRP、AP)、或未標誌及Low Endotoxin/Azide Free(LE/AF)等標定。
SouthernBiotech 抗小鼠二抗(anti-mouse secondary antibodies)是經過廣泛表徵的親和純化(affinity-purified)的多克隆(polyclonal)或單克隆抗體(monoclonal antibodies),對小鼠免疫球蛋白重鏈類別(mouse immunoglobulin heavy chain classes)和亞類(subclasses)、輕鍊(light chains)和片段(fragments)具有特異性(specificities)。SouthernBiotech提供在山羊(goat)、兔(rabbit)、驢(donkey)和大鼠(rat)中產生的抗小鼠二抗。它們在檢測一抗(primary antibodies )方面很有價值,並且有多種形式可供選擇,包括未偶聯以及酶、生物素化和螢光偶聯物,用於 ELISA、蛋白質印跡(western blot)、流式細胞術(flow cytometry)、多重和免疫組織化學(immunohistochemistry)等分析。
由於單克隆抗體 ( monoclonal antibodies )將結合抗體靶標上的單個表位,因此它們具有高度特異性(specific),可提供出色的批次間一致性(lot-to-lot consistency),並且通常在文獻中得到很好的表徵。我們的多克隆抗小鼠二抗(polyclonal anti-mouse secondary antibodies)可以通過信號放大提高靈敏度(sensitivity),因為多個二抗可以與單個一抗結合。
選定的抗體已與來自其他物種的 IgG 和/或血清蛋白交叉吸附,以產生對這些物種具有最小交叉反應性(cross-reactivity)的抗體。使用對您的樣品物種具有最小交叉反應性的二抗非常重要。通過提供全面的多克隆(polyclonal)和單克隆(monoclonal)抗小鼠類(anti-mouse class)和亞類特異性二抗(例如 IgG1、IgG2b、IgM),可以在測定中同時檢測多種小鼠一抗。我們有廣泛的全分子和F(ab’)2 fragment 片段多克隆和單克隆抗小鼠抗體可供選擇,所有這些抗體都是內部生產和驗證的,可用於您的特定檢測。
Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

