組蛋白 H2AX ELISA KIT|Human H2AFX (Histone H2AX) ELISA Kit貨號 HUFI05171
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Human H2AFX (Histone H2AX) ELISA Kit (HUFI05171)貨號 HUFI05171
Human H2AFX (Histone H2AX) ELISA Kit
H2AX is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin.the gene encodes a 142-amino acid protein having a unique C-terminal sequence, QASQEY, which has been conserved from lower eukaryotes.Nijmegen breakage syndrome protein (NBS1) and phosphorylated H2AX (gamma-H2AX), which associate with irradiation-induced DNA double-strand breaks (DSBs), are also found at sites of V(D)J (variable, diversity, joining) recombination-induced DSBs. In developing thymocytes, NBS1 and gamma-H2AX form nuclear foci that colocalize with the T-cell receptor-alpha (TCRA) locus in response to recombination-activating gene-1 (RAG1) protein-mediated V(D)J cleavage.
人 H2AFX（組蛋白 H2AX）ELISA 試劑盒 (貨號HUFI05171)。該試劑盒使用Sandwich ELISA, Double Antibody。 可檢測樣品中的組蛋白 H2AX，如serum 、plasma 及其它生物體液(biological fluids)等。
Human H2AFX (Histone H2AX) ELISA Kit
| Product Name: | Human H2AFX (Histone H2AX) ELISA Kit |
| Product Code: | HUFI05171 |
| Size: | 96 Assays |
| Alias: | H2afx ELISA Kit, H2a ELISA Kit, x ELISA Kit, H2AFX ELISA Kit, H2AX ELISA Kit, Histone H2A.X ELISA Kit |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human H2AFX (Histone H2AX) concentrations in serum plasma and other biological fluids. |
| Sensitivity: | < 46.875pg/ml |
| Range: | 78.125-5000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human H2AFX (Histone H2AX) and the recovery rates were calculated by comparing the measured value to the expected amount of Human H2AFX (Histone H2AX) in samples. Enquire for more information. |
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human H2AFX (Histone H2AX) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Enquire for more information. |
| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | – |
