mini質體DNA萃取套組 試劑盒| Plasmid miniPREP Kit  貨號PDP01-0100 100個反應

PureDireX小量質體DNA (plasmid DNA, PDP01-0100) 試劑盒提供了一種快速，簡單，且成本有效地用於從培養的革蘭氏陽性(Gram positive)和革蘭氏陰性細菌細胞(Gram negative bacterial cells)分離質體DNA (plasmid DNA)的方法。該設計基於細菌細胞的鹼裂解(alkaline lysis)，然後在存在高濃度鹽的情況下將 DNA 結合到離心柱(spin column)的玻璃纖維基質(glass fiber matrix)上。使用該試劑盒純化的質體DNA (plasmid DNA)適用於各種常規應用，包括限制性內切酶消化(restriction enzyme digestion)、測序(sequencing)、文庫篩選(library screening)、體外轉譯  (in vitro translation)、細胞轉染(transfection)、連接(ligation)和轉化(transformation)。整個過程可以在15-20分鐘內完成。

最快的程序： 在 15-20 分鐘內完成
樣品： 最多4 ml細菌細胞
產量： 最多30μg質粒

Note: PureDireX / Purification Kits / Extraction Kit / Plasmid DNA
Categories: Plasmid DNA Purification System

 

Plasmid miniPREP Kit

Protocol Step 1 Bacterial Cells Harvesting

1. Transfer 1.5 ml bacterial culture to a microcentrifuge tube.

2. Centrifuge at 14,000 x g for 1 minute and discard the supernatant.

Step 2 Resuspend

1. Resuspend pelleted bacterial cells in 200 μl of the Buffer S1 (RNase A added).

Step 3 Lysis

1.Add 200 μl of the Buffer S2 and mix thoroughly by inverting the tube 10 times (do not vortex) and then stand at the room temperature for 2 minutes or until the lysate is homologous.

Step 4 Neutralization 1.Add 300 μl of the Buffer S3 and mix immediately and thoroughly by inverting the tube 10 times (Do not vortex). 2. Centrifuge at 14,000 x g for 3 minutes.

Step 5 Binding 1.Place a PM column in a Collection Tube. Apply the supernatant (from step 4) to the PM column by decanting or pipetting. 2. Centrifuge at 14,000 x g for 30 seconds, then discard the flow-through, and place the PM column back into the same collection tube.

Step 6 Wash 1.Add 400 μl of the Buffer W1 into the PM column. 2. Centrifuge at 14,000 x g for 30 seconds. 3. Discard the flow-through and place the PM column back into the same collection tube. 4.Add 600 μl of the Buffer W2 (Ethanol added) into the PM column. 5. Centrifuge at 14,000 x g for 30 seconds. 6. Discard the flow-through and place the PM column back into the same collection tube. 7. Centrifuge at 14,000 x g again for 2 minutes to remove the residual Buffer W2.  Step 7 Elution 1. To elute DNA, place the PM column in a clean 1.5 ml microcentrifuge tube. 2.Add 50~200 μl of the Buffer E or H2O (pH between 7.0 and 8.5) to the center of each PM column, let it stand for 2 minutes, and centrifuge at 14,000 x g for 2 minutes. ＊Check the buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.

乾浴加熱水浴槽 |  Finnpipette F3 微量分注器 |  試管振盪器 Vortex Mixer  |  Genereach迷你型微量離心機  |   ELISA儀器

bio-helix.com

Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。

在改善生活質量和實現科學卓越的願望的驅使下，Bio-Helix以最高的誠信標準運營。還將這種精神延伸到我們的服務和全球價值中，以提供給世界各地的客戶。多樣化的員工隊伍和協作網絡形成了公司文化的完美結合。 Bio-Helix 不僅是一家機會均等的公司，而且還努力提供友好和成長的環境。

CAT#	NAME	Size
BP001CU	BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442)	Set
PMI09-0500	IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa)	500 μl
PMI11-0500	IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa)	500 μl
PMB01-0500	Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa)	500 μl
PMB10-0500	Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa)	500 μl
PMB11-0500	Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa)	500 μl
PMB12-0500	Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa)	500 μl
PMB13-0500	Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa)	500 μl
PMU12-0500	UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa)	500 μl
CCH321-B100ML	UltraScence Pico Plus Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
CCH345-B100ML	UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
CCH365-B100ML	UltraScence Femto Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
CCH375-B100ML	UltraScence Femto Plus Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
PS002-B500ML	COOMASSIEnano Protein Staining Solution, 500ml	500 ml
PS003-B500ML	Ponceau S Protein Staining Solution	500 ml
BS001-B500ML	OneStep Blocker Western Blocking Solution and Signal Enhancer	500 ml
TM001-B200ML	TMB ELISA Substrate	100 ml (50ml+50ml)
DM001-R500	BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps)	500 μl
DM003-R500	BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps)	500 μl
DM012-R500	BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps)	500 μl
DM015-R500	BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps)	500 μl
DMF12-0100	OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye)	600 μl
DMF13-0100	OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye)	600 μl
LD011-1000	Prime Juice Preloading Fluorescent Stain	1 ml
LD003-0500	Novel Green Plus (20,000X) DNA Staining Reagent	500 μl
AGT002-0500	Agarose Tablets, 110 pcs	0.5g x 110
MB755-0100	Agarose Powder 100g -Molecular Biology Grade	100 g
MB755-0500	Agarose Powder 500g -Molecular Biology Grade	500 g
MB101-0500	Taq DNA Polymerase	500U/100μl
DP001-0100	nanoTaq™ Hot-Start DNA Polymerase	100 rxns 500U/100μl
DN001-0250	100 mM dNTP Set	4 x 250μl
DN0010	10 mM dNTP Mix	1 ml
MB200-P100	2X PCR SuperMix	100 rxns 2 x 1.25mL
MBA01-0100	OmniPCR Supermix w Fluorescent dye	100 rxns (2.5ml)
RK001-0050	RScript™ cDNA Synthesis Kit	50 rxns
RT001-0050	RScript™ Reverse Transcriptase 10,000U	50 rxns 10,000U/50μl
RT001-0250	RScript™ Reverse Transcriptase 50,000U	250 rxns 50,000U/250μl
RI001-0125	RIBOAssure™ RNase Inhibitor 5,000U	125 rxns 5,000U/125μl
QPR01-0100	PanProbes™ One-Step RT-qPCR Kit	100 reactions (20 μl vol)
QPD01-0100	PanProbes™ Universal qPCR MasterMix	100 reactions (20 μl vol)
QSR01-0100	PanGreen™ One-Step RT-qPCR Kit	100 reactions (20 μl vol)
QSD01-0100	PanGreen™ Universal SYBR® Green Mastermix	100 reactions (20 μl vol)
PDP01-0100	Plasmid miniPREP Kit	100 rxns
PDP02-0020	Plasmid midiPREP Kit	20 rxns
PDP03-0010	Plasmid maxiPREP Kit	10 rxns
PDC01-0100	PCR Clean-Up & Gel Extraction Kit	100 rxns
PDC02-0100	Genomic DNA isolation dual kit	100 rxns
PDC05-0100	Dual Genomic DNA Isolation Kit (Plant)	100 rxns
PDC06-0100	Dual Genomic DNA Isolation Kit(Tissue)	100 rxns
PDC09-0100	Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)	100 rxns
PDC10-0100	Genomic DNA Isolation Kit (Plant)	100 rxns
PDC11-0100	Genomic DNA Isolation Kit (Tissue)	100 rxns
PDC12-0100	Genomic DNA Isolation Kit (Paraffin-embedded tissue)	100 rxns
PDM01-0100	Mbead Buffy Coat Genomic DNA Kit	100 rxns
PDM02-0100	Mbead Tissue Genomic DNA Kit	100 rxns
PDM03-0100	Mbead Bacteria Genomic DNA Kit	100 rxns
PDM05-0100	Mbead Plant Genomic DNA Kit	100 rxns
PDM06-0100	Mbead Blood/Cell Genomic DNA Kit	100 rxns
PDR01-0100	Genomic DNA Isolation Reagent	100 rxns
PDR02-0100	Plant Genomic DNA Isolation Reagent	100 rxns
PDR05-0100	Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue)	100 rxns
PDC04-0100	Total RNA Isolation Kit (Blood/Cultured Cell/Fungus)	100 rxns
PDC07-0100	Total RNA Isolation Kit (Plant)	100 rxns
PDC08-0100	Total RNA Isolation Kit(Tissue)	100 rxns
PDR03-0100	Total RNA Isolation Reagent	100 rxns
PDR04-0100	Plant Total RNA Isolation Reagent	100 rxns
PDC03-0100	Virus Nucleic Acid Isolation Kit	100 rxns
PDM04-0100	Mbead Virus Genomic Nucleic Acid Kit	100 rxns
QP019M-0050	COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)	50 rxns
QP019T-0100	COVID-19 RT-qPCR Detection Kit Plus (TFDA)	100 rxns
QP019-0100	SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO)	100 rxns