脂肪組織/培養脂肪細胞核和細胞質分離試劑盒| Invent貨號AN-029
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
產品名稱: 用於脂肪組織/培養脂肪細胞核和細胞質分離試劑盒（20 個反應）| Invent貨號AN-029 （20 個反應）
Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes (20 preps)
產品介紹: 脂肪細胞(Adipocytes)是體內主要的能量儲存部位，它們也具有重要的內分泌(endocrine)功能。因此，了解脂肪細胞的發育(development)和功能(function)對於了解生理(physiological)和病理(pathological)條件下的代謝穩態(metabolic homeostasis)至關重要。將細胞成分分成單核(single nucleus)和細胞質(cytosolic fraction)部分是實驗室中的常見做法。然而，當涉及到脂肪細胞(adipocytes)時，由於脂肪細胞的脂滴(lipid droplets)濃度高和蛋白質含量低，因此分離這兩個部分要困難得多。文獻中報導的方法既乏味又耗時，並且需要多達 50 克的組織。該試劑盒提供了一種從脂肪組織(adipose tissues)中獲得高純度單核(single nuclei)的非常快速和簡單的方法，最重要的是，只需要毫克量的組織，就可以從小動物和活檢樣本中分離細胞核(nuclei)和細胞質(cytosol)。分離出的完整細胞核有許多應用，例如單核 RNA 測序(single nucleus RNA sequencing)、SDS-PAGE()、免疫印跡(immunoblotting)、ELISA、IP、蛋白質定位(protein localization)、凝膠遷移率變化測定(gel mobility shift assays)、2-D 凝膠(2-D gels)和其他應用。
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性。無須長時間等待、免除重複性的wash步驟。
引用文獻
1. Rajbhandari, P., Arneson, D., Feng, A. C., Ahn, I. S., Diamante, G., Zaghari, N., … & Smale, S. T. (2019). Single Cell Analysis Reveals Immune Cell-Adipocyte Crosstalk Regulating the Transcription of Thermogenic Adipocytes. bioRxiv, 669853.
Kit includes:
| Items | Quantity |
| N/C Buffer | 15 ml |
| 1.5 ml Eppendorf Tubes | 20 units |
| Pestle for 1.5 ml Eppendorf Tubes | 2 units |
| Filter Cartridge | 20 units |
| 2 ml Collection Tubes | 20 units |
| Product Name | Catalog No. |
| Minute TM Total Protein Extraction Kit for Animal Cultured Cells/Tissues (50 Tests) | SD-001/SN-002 |
| Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests) | SC-003 |
| Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) | SN-006 |
| Minute TM Plasma Membrane Protein Isolation Kit (50 Tests) | SM-005 |
| Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests) | SM-005-p |
| Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests) | MP-007 |
| Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests) NEW! | MM-038 |
| Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) | SD-008/SN-009 |
| Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) | SN-010 |
| Minute TM Chloroplast Isolation Kit (50 Tests) | CP-011 |
| Minute TM Bacterial Total Protein Extraction Kit (50 Tests) | SB-004 |
| Minute TM Single Cell Isolation Kit (50 Tests) | SC-012 |
| Minute TM Nuclear Envelope Protein Extraction Kit (50 Tests) | NE-013 |
| Minute TM Histon/DNA Binding Protein Extraction Kit (50 Tests) | HP-014 |
| Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YT-015 |
| Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YD-016 |
| Minute™ Yeast Mitochondria Enrichment Kit (50 tests) | YM-017 |
| Minute™ Plant Microsomal Membrane Extraction Kit (50 Tests) | MM-018 |
| Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 tests) | PN-019 |
| MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests) | HD-021 |
| MinuteTM Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Tests) | AT-022 |
| MinuteTM Adipose Tissue Fractionation Kit (20 Tests) | AF-023 |
| MinuteTM Detergent-Free Nuclei Isolation Kit (20 Tests) | NI-024 |
| MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps) NEW! | BN-020 |
| MinuteTM Single Nucleus Isolation Kit for Tissues/Cells (20 preps) NEW! | SN-047 |
| MinuteTM Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests) | FE-025 |
| MinuteTM Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG) | EI-027 |
| MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG) | SE-030 |
| MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 tests) | NT-032 |
| MinuteTM Lysosome Isolation Kit (20 tests) | LY-034 |
| MinuteTM ER Enrichment Kit (20 tests) | ER-036 |
| MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) NEW! | LR-039 |
| MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) NEW! | LR-042 |
| MinuteTM Cytosolic Proteasome Enrichment Kit (20 tests) NEW! | PT-040 |
| MinuteTM Nuclear Proteasome Enrichment Kit (20 tests) NEW! | PN-041 |
| MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW! | PF-045 |
| MinuteTM Native Protein Precipitation Kit from Saliva (20 tests) NEW! | SV-043 |
| MinuteTM Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW! | ML-044 |
| MinuteTM Rubisco Depletion Kit (20 tests) NEW! | RD-046 |
| MinuteTM Total Protein Extraction Kit for Skin Tissue (50 tests) | SA-01-SK |
| MinuteTM Total Protein Extraction Kit for Bone Tissue (50 tests) | SA-02-BT |
| MinuteTM Total Protein Extraction Kit for Blood Vessels (50 tests) | SA-03-BV |
| MinuteTM Total Protein Extraction Kit for Tendons (50 tests) | SA-04-TD |
| MinuteTM Total Protein Extraction Kit for Intestines and Mesentery (50 tests) | SA-05-IM |
| MinuteTM Total Protein Extraction Kit for Muscles (50 tests) | SA-06-MS |
| MinuteTM Total Protein Extraction Kit for Insects (50 tests) | SA-07-IS |
蛋白質萃取熱門商品 脂肪組織蛋白質萃取套組 | 質膜蛋白萃取套組