qPCR Adeno-Associated Virus Titration (Titer) Kit | AAV病毒快速純化套組G931
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qPCR Adeno-Associated Virus Titration (Titer) Kit 病毒快速定量套組 (不需要額外的裂解)
abm的qPCR AAV滴定試劑盒是通過qPCR進行的一步分析，不需要額外的裂解，可以在一小時內完成。與同類試劑盒相比，該試劑盒旨在提供高靈敏度和特異性，可確保最小的非特異性背景和更好的整體性能。 ROX參考染料與MasterMix分開提供，使該試劑盒與大多數qPCR儀器通用兼容。
qPCR Adeno-Associated Virus Titration (Titer) Kit-G931
| PRODUCT COMPONENT | QUANTITY |
|---|---|
| BlasTaq™ 2X qPCR Titer MasterMix | 1.25 ml |
| Primer Mix | 100 rxn (200 µl) |
| Standard Control DNA | 50 µl |
| DNase I Reaction Mix | 2 x 1.0 ml |
| ROX Reference Dye | 15 µl |
| Nuclease-Free H2O | 2 x 1.0 ml |
abm’s qPCR AAV Titer Kit is a one-step assay by qPCR which does not require additional lysis, and can be completed under one hour. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.
Data Analysis Plot Ct value (Y-axis, linear scale) vs. Virus titer (X-axis, logarithmic scale). Generate a logarithmic regression using the four (4) Standard Control DNA dilutions to determine the unknown virus sample titer using y = mx + b from the trendline equation. The R2 value should be > 0.95 to justify the proper assay setup. Note to include the dilution factor in the final calculation (i.e. if you diluted your purified viral sample 1/100 in Step 1 and 1/10 in Step 2, then the titer of the unknown sample should be multiplied by a factor of 100*10).
| MACHINE TYPE | MACHINE |
|---|---|
| High ROX Machines |
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| Low ROX Machines |
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| No ROX machines |
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