HSP70-Ab熱休克蛋白70抗體ELISA試劑盒| 貨號KTE62472

Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit

熱休克蛋白70（Heat shock protein 70, HSP70）在許多類型的腫瘤(tumors)中均過表達(overexpressed)，並被認為與腫瘤的進展(tumor progression)密切相關。雞HSP70（chHSP70）異種DNA疫苗(xenogeneic DNA vaccine)，可在CTVT（beagle canine transmissible venereal tumor , CTVT）模型中引發免疫應答。 CTVT犬接受空載體疫苗接種且未進行任何治療（CW）作為對照。結論是，異種chHSP70 DNA疫苗先後破壞了對犬HSP70的腫瘤耐受性，並表現出有希望的預防活性，從而抑制了表達HSP70的腫瘤的生長。 70 kilodalton的熱激蛋白（Hsp70s）是一個普遍表達的熱激蛋白家族(ubiquitously expressed heat shock proteins)。幾乎所有活生物體中都存在具有相似結構的蛋白質。 Hsp70s是細胞折疊蛋白質機制的重要組成部分，有助於保護細胞免受壓力。

產品描述：該人類熱激蛋白70抗體（HSP70-Ab）ELISA試劑盒 (貨號KTE62472)採用了競爭性酶免疫分析技術。該試劑盒中提供的微量滴定板已預先塗有對HSP70-Ab特異的抗體。然後將標準品或樣品與辣根過氧化物酶（HRP）共軛的HSP70-Ab加入適當的微量滴定板孔中並進行孵育。在具有抗體的HRP標記的HSP70-Ab和未標記的HSP70-Ab之間啟動競爭性抑制反應。將底物溶液添加到孔中，並且顏色與樣品中HSP70-Ab的數量相反。停止顯色並測量顏色強度。Detection range is 30pg/ml – 480pg/ml。limit of detection value is 1 pg/ml。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

• Product name

Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit

• Reactivity

Human

• Applications

ELISA

• Application notes

This Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP70-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated HSP70-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled HSP70-Ab and unlabeled HSP70-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of HSP70-Ab in the sample. The color development is stopped and the intensity of the color is measured.

• Detection method

Colorimetric

• Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

• Assay type

Sandwich ELISA (quantitative)

• Assay duration

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

• Kit components
• Human Heat Shock Protein 70 antibody microplate
  • Human Heat Shock Protein 70 antibody standard
  • Human Heat Shock Protein 70 antibody detect antibody
  • Streptavidin-HRP
  • Standard diluent
  • Assay buffer
  • HRP substrate
  • Stop solution
  • Wash buffer
  • Plate covers
• Features & Benefits

Human Heat Shock Protein 70 antibody (HSP70-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human HSP70-Ab. No significant cross-reactivity or interference between Human HSP70-Ab and analogues was observed.

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。