Anti-COVID-19 & SARS-CoV S glycoprotein [CR3022], Rabbit IgG, Kappa抗體 |貨號Ab01680-23.0   

COVID19 , S蛋白;SARS-CoV S蛋白;S糖蛋白;E2; SARS冠狀病毒; SARS-CoV-2;SARS CoV 2;2019-nCoV

產品介紹: COVID19抗體與SARS-CoV和SARS-CoV-2結合,具有高親和力(PMID:16796401 = 32065055)。該抗體結合的初始特徵由ELISA進行,表明診斷檢測的發展潛力,無論是病毒捕獲檢測,還是作為血清學測定中測量病毒暴露免疫反應的控制。人類IgG1、IgG3、IgM和IgA等值型可用於類比COVID19(Amanat等人,2020年)中看到的抗體反應。人類IgG2和IgG4亞型,也出現在COVID-19患者的一小部分,也可以調查他們在應對SARS-CoV-2中的作用。最初的人類IgG1版本的抗體與另一種非抗性SARS抗體CR3014協同工作,是SARS-CoV感染被動免疫預防的潛在候選者(Meulen等人,2006年)。據報導,原始抗體(人類IgG1)還結合2019-nCoV RBD(KD為6.3 nM)。這種抗體被歸結為一種作為治療劑,單獨或與其他中和抗體聯合用於治療2019-nCoV感染的潛力(Tian等人,2020年)。

Purification: Protein A affinity purified

Supplied in: PBS with 0.02% Proclin 300.

Storage Recommendation: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Concentration: 1 mg/ml.

免疫原:原始單克隆抗體是通過對暴露於SARS-CoV的患者外周血淋巴細胞進行測序產生的。特異性:這種抗體將SARS-CoV尖峰蛋白的S1域中的氨基酸318-510與SARS-CoV-2(COVID-19)尖峰蛋白結合。抗體還與P462L替代S318-510片段的SARS尖峰蛋白結合。結合的表上皮只能在尖峰蛋白的「開放」匯結中獲得(Joyce等人,2020年)

Antibody first published in: ter Meulen et al. Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants PLoS Med. 2006 Jul; 3(7): e237 PMID:16796401
Note on publication: Describes the discovery and original characterization of this antibody.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (Ab01680-10.0) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (The Native Antigen Company) at concentrations of 5 µg/ml. A 3-fold serial dilution from 125 ng/ml was performed using Ab01680-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (Ab01680-10.0) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (The Native Antigen Company) at concentrations of 5 µg/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using Ab01680-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (Ab01680) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)). ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) (Native Antigen) at concentrations of 5 µg/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using Ab01680-10.0; from 370 ng/ml for Ab01680-16.0 and from 10000 ng/ml for Ab01680-15.0 and Ab01680-11.0. Human IgM, human IgA and human IgG2 were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.

產品貨號	Mouse target	抗體clone	Original Antibody format
Ab01052	4-1BB (CD137)	LOB12.3	Rat IgG1
Ab01054	4-1BBL (CD137L)	AT113-2	Rat IgG1
Ab00881	4-1BBL (CD137L)	TKS-1	Rat IgG2a
Ab01027	BTLA	HMBT-6B2	Hamster IgG
Ab00670	CD27	LG.3A10	Hamster IgG
Ab00895	CD27	RM27-3E5	Rat IgG2a
Ab00286	CD28	E18	Mouse IgG2b
Ab00285	CD28	D665	Mouse IgG1
Ab01109	CD40	3/23	Rat IgG2a
Ab01087	CD40L (CD154)	MR1	Hamster IgG
Ab00816	CD70	TAN 1-7	Rat IgG2a
Ab01056	CD70	TAN1-6	Rat IgG2a
Ab01028	CD80 (B7-1)	RM80	Rat IgG2a
Ab01029	CD86 (B7-2)	PO.3	Rat IgG2b
Ab01089	CD153	RM153	Rat IgG2b
Ab01297	CD96	6A6	Rat IgG2a
Ab00894	CTLA-4	9H10	Hamster IgG2
Ab01018	CTLA-4	9D9	Mouse IgG2b
Ab01088	GAL-9	RG9-35	Rat IgG2a
Ab01060	GITR	DTA-1	Rat IgG2a
Ab00374	GITR	YGITR 860.103.5	Rat IgG2b
Ab00631	GITR	YGITR765	Rat IgG2b
Ab00630	GITRL	YGL386	Rat IgG1
Ab01026	HVEM	HMHV-1B18	Hamster IgG
Ab00814	ICOS	7E.17G9	Rat IgG2b
Ab00882	ICOSL	HK5.3	Rat IgG2a
Ab01295	LAG3	C9B7W	Rat IgG1
Ab01295	LAG3	C9B7W	Rat IgG1
Ab00110	OX40 (CD134)	OX86	Rat IgG1
Ab00564	OX40L	OX-89	Rat IgG1
Ab00813	PD-1	RMP1-14	Rat IgG2a
Ab01017	PD-1H (VISTA)	mam82	Mouse IgG1
Ab01016	PD-1H (VISTA)	MH5A	Hamster IgG
Ab00373	PD-L1	YDC 127.1.1	Rat IgG2a
Ab00883	PD-L2	TY25	Rat IgG2a
Ab00875	PDPN (MAP-Tag)	PMab-1	Rat IgG2a
Ab00973	TIGIT	4D4	Hamster IgG
Ab01258	TIGIT	1B4	Mouse IgG1
Ab01032	TIM-1	3B3	Rat IgG2a
Ab01110	TIM-2	RMT2-14	Rat IgG2a
Ab01057	TIM-3	2C12	Rat IgG1
Ab01058	TIM-4	5G3	Rat IgG1
Ab01059	TIM-4	3A1	Rat IgG1