Minute™植物微粒體膜提取試劑| Invent貨號MM-018
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特點
產品名稱: Minute™植物微粒體膜提取試劑盒（50個分析）| Invent貨號MM-018
Minute™ Plant Microsomal Membrane Extraction Kit (50 Preps)
產品介紹: 從植物組織(plant tissues)中分離微粒體膜(microsomal membranes)是一種常見方法。植物細胞裂解物的微粒體部分(Microsomal fraction)是許多植物研究項目中關注的焦點，並且據信富含細胞質膜(plasma membranes)，內質網(endoplasmic reticulum)，高爾基體(Golgi apparatus)，液泡膜(vacuolar membranes)和膜系統(membrane system)的其他組件。用於微粒體級分分離的傳統方法(Traditional methods)包括差分沉澱法(differential pelleting)，其中膜級分需要離心步驟。微粒體級分的分離(microsomal fraction isolation)通常需要大量的起始原料，並且具有繁瑣的超速離心步驟。 MM-018提供了一種簡單，快速、user-friendly的方法，可使用少量起始原料來提取微粒體膜 (microsomal membrane extraction)。在操作過程中，將水溶性胞質蛋白(water-soluble cytosolic proteins)去除，並在低速微量離心機中用優化的緩衝液提取水不溶性微粒體部份，尤其是質膜部份(plasma membrane fraction)。Minute™植物微粒體膜提取試劑盒 (貨號MM-018)，無需超速離心(without ultracentrifugation)即可在約一小時內從植物組織中分離出天然微粒體蛋白。蛋白質產量為100-200 µg /樣品。
| Minute™植物微粒體膜提取試劑盒 | Quantity |
| Buffer A | 25 ml |
| Buffer B | 10 ml |
| Filter Cartridges | 50 units |
| 2.0 ml Collection Tubes with Caps | 50 units |
| Plastic Rods | 2 units |
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性。無須長時間等待、免除重複性的wash步驟。
文獻引用
- Kim, S., Choi, Y., Kwon, C., & Yun, H. S. (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. Journal of integrative plant biology.
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| Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests) | SC-003 |
| Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) | SN-006 |
| Minute TM Plasma Membrane Protein Isolation Kit (50 Tests) | SM-005 |
| Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests) | SM-005-p |
| Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests) | MP-007 |
| Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests) NEW! | MM-038 |
| Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) | SD-008/SN-009 |
| Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) | SN-010 |
| Minute TM Chloroplast Isolation Kit (50 Tests) | CP-011 |
| Minute TM Bacterial Total Protein Extraction Kit (50 Tests) | SB-004 |
| Minute TM Single Cell Isolation Kit (50 Tests) | SC-012 |
| Minute TM Nuclear Envelope Protein Extraction Kit (50 Tests) | NE-013 |
| Minute TM Histon/DNA Binding Protein Extraction Kit (50 Tests) | HP-014 |
| Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YT-015 |
| Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) | YD-016 |
| Minute™ Yeast Mitochondria Enrichment Kit (50 tests) | YM-017 |
| Minute™ Plant Microsomal Membrane Extraction Kit (50 Tests) | MM-018 |
| Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 tests) | PN-019 |
| MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests) | HD-021 |
| MinuteTM Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Tests) | AT-022 |
| MinuteTM Adipose Tissue Fractionation Kit (20 Tests) | AF-023 |
| MinuteTM Detergent-Free Nuclei Isolation Kit (20 Tests) | NI-024 |
| MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps) NEW! | BN-020 |
| MinuteTM Single Nucleus Isolation Kit for Tissues/Cells (20 preps) NEW! | SN-047 |
| MinuteTM Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests) | AN-029 |
| MinuteTM Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests) | FE-025 |
| MinuteTM Total Protein Extraction Kit for Mass Spectrometry (50 Tests) | MS-026 |
| MinuteTM Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG) | EI-027 |
| MinuteTM Endosome Isolation and Cell Fractionation Kit (20 Tests) | ED-028 |
| MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG) | SE-030 |
| MinuteTM Cell Suspension Isolation from Fresh/Frozen Tissues (50 tests) | CS-031 |
| MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 tests) | NT-032 |
| MinuteTM Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests) | NM-033 |
| MinuteTM Lysosome Isolation Kit (20 tests) | LY-034 |
| MinuteTM High Fidelity ER Isolation Kit for Liver Tissue (20 tests) | ER-035 |
| MinuteTM ER Enrichment Kit (20 tests) | ER-036 |
| MinuteTM Golgi Apparatus Enrichment Kit (20 tests) | GO-037 |
| MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) NEW! | LR-039 |
| MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests) NEW! | LR-042 |
| MinuteTM Cytosolic Proteasome Enrichment Kit (20 tests) NEW! | PT-040 |
| MinuteTM Nuclear Proteasome Enrichment Kit (20 tests) NEW! | PN-041 |
| MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW! | PF-045 |
| MinuteTM Native Protein Precipitation Kit from Saliva (20 tests) NEW! | SV-043 |
| MinuteTM Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW! | ML-044 |
| MinuteTM Rubisco Depletion Kit (20 tests) NEW! | RD-046 |
| MinuteTM Total Protein Extraction Kit for Skin Tissue (50 tests) | SA-01-SK |
| MinuteTM Total Protein Extraction Kit for Bone Tissue (50 tests) | SA-02-BT |
| MinuteTM Total Protein Extraction Kit for Blood Vessels (50 tests) | SA-03-BV |
| MinuteTM Total Protein Extraction Kit for Tendons (50 tests) | SA-04-TD |
| MinuteTM Total Protein Extraction Kit for Intestines and Mesentery (50 tests) | SA-05-IM |
| MinuteTM Total Protein Extraction Kit for Muscles (50 tests) | SA-06-MS |
| MinuteTM Total Protein Extraction Kit for Insects (50 tests) | SA-07-IS |