Annexin V-APC細胞凋亡檢測  | 貨號10040-11   100個反應

ApoScreen® Annexin V Apoptosis Kit-APC 不包括細胞核染劑PI ( SBA貨號10044-01) 或7-AAD (SBA貨號10042-01)

Apoptosis 細胞凋亡介紹-Annexin V分析

細胞凋亡是指為維持有機體內環境穩定，由基因控制的細胞自主的有序的死亡。正常情況下，任何細胞在形成過程中發生的異常都會通過凋亡消除。例如，體內的癌細胞增長為腫瘤的過程會受細胞凋亡的引導而被抑制。然而，在抑癌基因p53出現問題時，凋亡就不會誘導發生，從而導致癌細胞的不斷增長。細胞凋亡可以通過細胞形態的變化或生物化學的變化來檢測。目前常用的指標有caspase活性分析、DNA片段分析、及磷脂醯絲氨酸phosphatidylserine (PS)的外翻等實驗。

Day old BALB/c mouse bone marrow cells were stained with Annexin V-CY5 (SB Cat. No. 10040-15) with and without Propidium Iodide (SB Cat. No. 10044-01).

Annexin V 細胞凋亡偵測原理

Annexin V細胞染色，用於檢測細胞凋亡早期的細胞膜變化。在細胞凋亡早期，膜磷脂醯絲氨酸phosphatidylserine (PS)由脂膜內側翻向外側。膜聯蛋白V (Annexin V)是一種Ca離子依賴性磷脂結合蛋白，可以與磷脂醯絲氨酸phosphatidylserine (PS) 特異結合。用綠色螢光FITC標記或PE標誌的Annexin V通過流式細胞儀或螢光顯微鏡可以檢測到細胞凋亡的發生。結合細胞核染劑PI( SBA貨號10044-01)或7-AAD (SBA貨號10042-01)，利用流式細胞儀或螢光顯微鏡可偵測偵測細胞凋亡現象及其凋亡分期。碘化丙啶(Propidium Iodide, PI, SBA貨號10044-01)是一種核酸染料，PI只能透過凋亡晚期和死細胞的細胞膜，因此Annexin V和PI結合使用，可以區分凋亡早晚期的細胞及死細胞。

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。

Description	ApoScreen® Annexin V (SB Cat. No. 10040) is a recombinant protein that is expressed in E. coli. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.
Source	E. coli
Buffer Formulation	Annexin V Binding Buffer – Contains 0.1% sodium azide and proprietary ingredients
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Flow Cytometry – Quality tested 1-25 Immunohistochemistry-Whole Mount – Reported in literature 26 Immunocytochemistry – Reported in literature 6,27
Recommended Dilutions	Please refer to product specific Technical Bulletin

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

Cat. No.	Product Description	Size
10010-02	ApoScreen® Annexin V Apoptosis Kit-FITC	100 tests
10010-09	ApoScreen® Annexin V Apoptosis Kit-PE	100 tests
10039-01	ApoScreen® Annexin V-UNLB	0.1 mg
10039-02	ApoScreen® Annexin V-FITC	100 tests
10039-08	ApoScreen® Annexin V-BIOT	100 tests
10040-01	ApoScreen® Annexin V-UNLB	0.1 mg
10040-02	ApoScreen® Annexin V-FITC	100 tests
10040-08	ApoScreen® Annexin V-BIOT	100 tests
10040-09	ApoScreen® Annexin V-PE	100 tests
10040-11	ApoScreen® Annexin V-APC	100 tests
10040-15	ApoScreen® Annexin V-CY5	100 tests
10042-01	ApoScreen® 7-Aminoactinomycin D (7-AAD)	100 tests
10044-01	ApoScreen® Propidium Iodide (PI)	100 tests
10045-01	ApoScreen® Annexin V Binding Buffer 10X	50 mL

References

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