曲狀桿菌PCR檢測套組| PCR-Campylobacter Detection Kit貨號K1453-96

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多年來,食源性疾病的發病率有所增加,並導致全球主要的公共衛生問題。 食源性病原體可以在各種食物中找到,檢測食源性病原體以提供安全的食物供應和預防食源性疾病非常重要。 BioVision提供PCR的檢測試劑盒,快速,準確和具有成本效益用於農業食品的食源性病原體檢測。
Advantages & Features:
• Easy and Ready-to-use Kit.
• Fast: Results in less than 24 hours with minimal handling.
• Reliable and highly specific results.
• Versatile: Validated for most used Thermal Cyclers.
• High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility.
PCR-Campylobacter Detection Kit
產品描述: 曲狀桿菌(Campylobacter)是全世界最常造成細菌性腸胃炎的人類致病菌之一,是旅遊者下痢常見病因。曲狀桿菌症為全世界性人畜共通傳染病,是常見的細菌性食物媒介疾病,只需500個細菌就可以致病。曲狀桿菌(Campylobacter)為革蘭氏陰性 (G(-)) 桿菌。具有鞭毛為微好氧菌。最適合生長的溫度是在42~45℃之間,低於30℃或者高於47℃則無法生長。(資料來源FDA網站)。BIOVIOSION提供 PCR-Campylobacter檢測試劑盒是通過real time PCR從食品和環境樣品中,快速且特異可靠地檢測嗜熱曲狀桿菌(空腸曲狀桿菌,大腸桿菌,C.upaliensis和C.lari)的理想工具。該試劑盒包括ready-to-use 即用型PCR MasterMix所需的所有試劑。優化之MasterMix含有緩衝液(Buffer)、dNTPs、熱啟動DNA聚合酶(Hot-start DNA Polymerase)、DNA-free water、MgCl2和內部擴增對照(IAC),其檢測表明不存在PCR抑制劑(PCR inhibitors)。用於擴增IAC以及擴增靶基因的引物和探針在MasterMix中。FAM標記探針用於檢測靶基因,而用JOE螢光染料標記探針用於檢測IAC。該試劑盒還包括陽性對照和陰性對照。提供陽性對照以證明PCR擴增與所提供的組分有效地起作用。為了確認沒有污染,每次使用試劑盒時都應包括陰性對照反應。在PCR檢測之前,包括DNAready Lysis Buffer從樣品中提取DNA。
| Cat # +Size | K1453-96 |
| Size | 96 Rxns |
| Kit Summary | PCR Campylobacter Detection Kit provides a quick, reliable, sensitive, and highly specific method to detect Campylobacter species from food and environmental samples in a single reaction tube by performing a real time PCR. |
| Detection Method | Real time PCR |
| Species Reactivity | C. jejuni, C. coli, C. upsaliensis and C. lari, Campylobacter species |
| Applications | An ideal tool for an specific, rapid, and reliable detection of thermophilic Campylobacter (C. jejuni, C. coli, C. upsaliensis and C. lari) from food and environmental samples by real time PCR. |
| Features & Benefits | • Amplification limit of one copy per reaction • Easy interpretation of the results from the amplification curves. • Highly conserved target genes • Reduce false negative results • Easy and Ready-to-use Kit. • The greatest exclusivity / inclusivity in the market for thermophilic Campylobacter species (C. jejuni, C. coli, C. upsaliensis and C. lari). • Without the need to use tedious conventional isolation and identification techniques. • Reliable and highly specific Results. • Internally validated with a wide variety of food samples. • Reliable results in c.a. 48 hours, including the enrichment step. |
| Kit Components | • PCR Negative Control • PCR Positive Control • PCR Master Mix |
