螢光素酶報導分析套組 | 貨號K801-200

BioVision’s Luciferase Reporter Assay Kit, K801

產品描述： 螢火蟲螢光素酶 (Firefly luciferase) 為具高靈敏度之報告基因，被用作研究基因調控和功能研究。與標準氯黴素乙酰轉移酶（chloramphenicol acetyltransferase , CAT）測定相比，螢光素酶 (Firefly luciferase)具1000倍的靈敏度。螢火蟲螢光素酶(Firefly luciferase)催化螢光素(luciferin)的氧化羧化(oxidative carboxylation)，是已知生物發光反應的最高效率的反應。冷光反應可以使用luminometer冷光儀器測量反應的light emission。 BioVision的螢光素酶報導分析試劑盒 (BioVision’s Luciferase Reporter Assay Kit, K801) 提供了一種檢測轉染的真核細胞中螢光素酶活性的簡單方法。為了在體外測量表達的螢光素酶，首先通過細胞裂解從轉染的細胞中提取螢光素酶。然後將CoA，ATP，Mg 2+和緩衝液加入裂解物中（包含CoA產生幾乎恆定的光發射，而不是典型的閃光動力學）。然後通過注射螢光素引發發光反應，並通過發光計記錄發射的光。通過在優化的緩衝溶液中提供CoA，以及ATP，Mg²，BioVision的螢光素酶報導分析試劑盒可確保最大靈敏度，一致的光輸出，以及使用多個樣品時的方便性和一致性。

Cat # +Size	K801-200
Size	200 assays
Detection Method	Luminescence
Species Reactivity	Mammalian
Applications	This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.
Features & Benefits	• The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.
Kit Components	• Substrate A • Substrate B • Cell Lysis Buffer
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.

引用文獻

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Martha Lappas. NOD1 and NOD2 Regulate Proinflammatory and Prolabor Mediators in Human Fetal Membranes and Myometrium via Nuclear Factor-Kappa B. Biol Reprod, Jul 2013; 89: 14.

Selvaraj Muthusamy et al., Estrogen receptor β and 17β-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer, PNAS, Dec 2011; 108: 20090 – 20094.

Veeraraghavan, J. et. al. Radiation-triggered Tumor Necrosis Factor (TNF) α-NF{kappa}B Cross-signaling Favors Survival Advantage in Human Neuroblastoma Cells. J. Biol. Chem., Jun 2011; 286: 21588 – 21600.