脂質過氧化比色測定 | 貨號K454-100

Lipid Peroxidation Colorimetric Assay Kit

產品描述：脂質過氧化(Lipid peroxidation)是一種影響細胞膜(cellular membranes) ，脂蛋白(lipoproteins)和其他含脂質分子(lipid-containing molecules) 的氧化損傷(oxidative damage)。該系列鏈反應(chain reactions)發生在高活性氧物質(reactive oxygen species)和細胞膜中的不飽和脂肪酸(unsaturated fatty acids)之間，這導致細胞損傷(cell damage)。源自多不飽和脂肪酸(polyunsaturated fatty acids) 的脂質過氧化物(Lipid peroxides)是不穩定的並且分解形成丙二醛（malondialdehyde , MDA）和4-羥基烯醛(4-hydroxyalkenals) 。 MDA的測量被廣泛使用並且被認為是脂質過氧化(lipid peroxidation) 的指標。傳統上，2-硫代巴比妥酸（2-Thiobarbituric acid , TBA）方法已廣泛用於樣品中MDA的比色檢測和測量。然而，該反應是相對非特異性的，因為游離的和蛋白質結合的MDA都與TBA反應。 BioVision的脂質過氧化檢測試劑盒(Lipid Peroxidation Assay Kit, K454) 使用一套專有試劑，可最大限度地減少其他脂質過氧化產物（包括4-hydroxyalkenals., 4-羥基烯醛）的干擾。該原理基於特定顯色試劑和MDA在45℃下的反應。該測定法易於實施，並產生強而穩定的比色信號（OD 586nm），其與樣品中MDA的量成比例。我們的分析可以檢測樣品中低至3 nmol的MDA。 MDA +顯色劑=髮色團（OD 586 nm）。

Cat # +Size	K454-100
Size	100 assays
Kit Summary	BioVision’s Lipid Peroxidation Assay Kit uses a proprietary set of reagents that minimizes the interference from other lipid peroxidation products including 4-hydroxyalkenals. The principle is based on the reaction between a specific chromogenic reagent and MDA at 45 °C. The assay is simple to perform, and yields a strong, yet stable colorimetric signal (OD 586 nm) that is proportional to the amount of MDA in samples. Our assay can detect as low as 3 nmol MDA in samples.
Detection Method	Colorimetric assay (OD 586 nm)
Species Reactivity	All
Applications	Measurement of lipid peroxidation in tissues and serum samples
Features & Benefits	• Specific • Simple procedure • Can detect as low as 3 nmol MDA in samples • Fast and convenient
Kit Components	• Diluent A • Chromogenic Reagent • MDA Standard • Diluent B • BHT