Mouse Anti-Chicken CD3-FITC抗體 | 貨號8200-02  規格1.0 mL  (0.5 mg/mL)

產品介紹:  CD3是T細胞受體相關CD3複合物 (T cell receptor-associated CD3 complex) 的成員。單株抗體CT-3在雞T細胞上識別至少三種Mr 20,19和17kDa的多肽(polypeptides)（其中兩種是N-糖基化， N-glycosylated）的複合物。 抗體還將來自洋地黃皂苷 (digitonin solubilized) 溶解的T細胞裂解物 (T cell lysates) 的90kDa的多肽共沉澱，其可以還原為Mr 50和40kDa的兩種多肽 (polypeptides) 。 Mouse Anti-Chicken CD3-FITC抗體，具FITC螢光標誌。免疫原為Chicken thymocytes and Ig- blood mononuclear cells針對Chicken/Pigeon CD3具特異性。Isotype為Mouse (BALB/c) IgG₁κ。Isotype Control Mouse IgG₁-FITC。緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02) and Mouse Anti-Chicken CD4-PE (SB Cat. No. 8210-09).

 

Clone	CT-3
Isotype	Mouse (BALB/c) IgG1κ
Isotype Control	Mouse IgG1-FITC
Immunogen	Chicken thymocytes and Ig– blood mononuclear cells
Specificity	Chicken/Pigeon CD3
Alternalte Name(s)	T3/TCR complex
Description	CD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.
Format/Conjugate	FITC (Fluorescein)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 1,5,10-18 Immunohistochemistry-Frozen Sections – Reported in literature 2-8 Immunohistochemistry-Paraffin Sections – Reported in literature 9 Immunoprecipitation – Reported in literature 1 Stimulation – Reported in literature 1
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796418

 References

1. Chen CH, Ager LL, Gartland GL, Cooper MD. Identification of a T3/T cell receptor complex in chickens. J Exp Med. 1986;164:375-80. (Immunogen, FC, IP, Stim)
2. Njenga MK, Dangler CA. Endothelial MHC class II antigen expression and endarteritis associated with Marek’s disease virus infection in chickens. Vet Pathol. 1995;32:403-11. (IHC-FS)
3. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)
4. Zheng W, Yoshimura Y. Organ-specificity of estrogen effects on the induction of immunocompetent cells in the chicken. J Poult Sci. 2001;38:41-9. (IHC-FS)
5. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. (FC, IHC-FS)
6. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. (IHC-FS)
7. Hansell C, Zhu XW, Brooks H, Sheppard M, Withanage S, Maskell D, et al. Unique features and distribution of the chicken CD83+ cell. J Immunol. 2007;179:5117-25. (IHC-FS)
8. Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. 2013;110:20170-5. (IHC-FS)
9. Solcan C, Solcan G, Cotea C. Immunotoxic action of ochratoxine A on lymphocytes from lymphoid tissues associated to gut mucosa in chickens. Bulletin UASVM Agriculture. 2010;67:283-90. (IHC-PS)
10. Koskinen R, Göbel TW, Tregaskes CA, Young JR, Vainio O. The structure of avian CD5 implies a conserved function. J Immunol. 1998;160:4943-50. (FC)
11. Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry. 2007;71A:925-33. (FC)
12. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
13. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. (FC)
14. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. (FC)
15. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)
16. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. (FC)
17. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. (FC)
18. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33. (FC, Pigeon Reactivity)

Cat. No.	Product Description	Clone	Size
8200-11	Mouse Anti-Chicken CD3-APC	CT-3	0.1 mg
8200-08	Mouse Anti-Chicken CD3-BIOT	CT-3	0.5 mg
8200-02	Mouse Anti-Chicken CD3-FITC	CT-3	0.5 mg
8200-01	Mouse Anti-Chicken CD3-UNLB	CT-3	0.5 mg
8200-30	Mouse Anti-Chicken CD3-Alexa Fluor® 488	CT-3	0.1 mg
8200-31	Mouse Anti-Chicken CD3-Alexa Fluor® 647	CT-3	0.1 mg
8200-27	Mouse Anti-Chicken CD3-Alexa Fluor® 700	CT-3	0.1 mg
8200-26	Mouse Anti-Chicken CD3-Pacific Blue™	CT-3	0.1 mg
8200-09	Mouse Anti-Chicken CD3-PE	CT-3	0.1 mg
8200-13	Mouse Anti-Chicken CD3-SPRD	CT-3	0.1 mg