Recombinant Human FGF1重組蛋白 | ABMGOOD貨號 Z100005
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生長因子(Growth Factors)和細胞因子(Cytokines Growth factors)-Recombinant Human FGF1
生長因子(Growth Factors)和細胞因子(Cytokines Growth factors)是天然存在的信號分子，是高等生物體細胞間通訊的關鍵而復雜的網絡的核心。這些分子通過在細胞之間傳遞調節信息，在決定所有細胞過程的命運方面發揮著至關重要的作用。雖然細胞因子主要與免疫系統的管家( housekeeping)有關，但生長因子(Growth Factors)在生物體的發育和成熟階段起著調節廣泛過程的作用。值得注意的是，生長因子(Growth Factors)信號傳導不受控制的“異常”激活通常與腫瘤發生有關。考慮到它們的關鍵作用，生長因子(Growth Factors)/細胞因子(Cytokines Growth factors)對於細胞生物學研究的許多方面都是不可或缺的，這並不奇怪。在傳統的細胞生物學實驗中，動物血清通常是正常細胞代謝所需的生長因子和營養物質的豐富但異質來源。然而，使用動物血清是不可取的，因為增加病毒、支原體和朊病毒污染的風險， 與血清採集相關的高成本和倫理問題，在非標準化介質的情況下難以準確解釋實驗結果其中可能包含生長抑制劑。
Recombinant Human FGF1 | 貨號 Z100005
Recombinant Human FGF1
產品介紹: FGF1重組蛋白 (基因Accession No. P05230), 來源為E. coli。純度> 95% (SDS-PAGE)，提供凍乾型式包裝。可應用於Cell culture; Activity Assays等不同研究。aFGF和bFGF顯示55％的同源性(homology)，但是對於少數功能域而言是有限的。 通過X射線晶體學(X-ray crystallography)揭示的aFGF的結構顯示出與IL1類似的折疊結構(folding structure)。 因為aFGF與bFGF結合相同的受體，所以aFGF顯示出或多或少相同的活性譜(spectrum of activities)。 然而，在類似的測定中，它通常比bFGF活性低。 FGF受體由基因家族編碼，該基因家族由至少四種受體酪氨酸激酶(receptor tyrosine kinases)組成，所述受體酪氨酸激酶transduce在與細胞生長和分化相關的各種發育和生理過程中重要的信號。 重組人FGF1是16kDa的單一多肽(single polypeptide)。
蛋白質保存型式
蛋白質溶於0.2 μm filtered solution in PBS為冷凍乾燥包裝。使用前將管子spin down後，重組於distilled water，濃度不低於0.1mg / mL。可以將該溶液稀釋到其他緩衝液中。避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。
| Alternative Names | aFGF, FGF-1, Heparin-binding growth factor 1, HBGF-1, Endothelial cell growth factor, ECGF-beta, FGFA, FGF1 |
| Description | aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. Recombinant Human FGF1 is a single polypeptide of 16 kDa. |
| Gene Symbol | FGF1 |
| Gene ID | 9158 |
| Accession No. | P05230 |
| Source | E. coli |
| Appearance | Lyophilized Powder |
| Molecular Weight | 16.0 kDa |
| Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The ED50 as determined by the dose-dependent stimulation of mouse fibroblasts was <0.5ng/ml |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° – 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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