Phi29 DNA Polymerase聚合酶 | 貨號E014 (1000U/100ul)

A highly processive DNA polymerase which exhibits a strong strand-displacement function

產品描述：Phi29 DNA聚合酶是一種高度加工的polymerase聚合酶 （高達70 kb以上），具有很強的strand displacement activity鏈換置換活性。phi29 DNA聚合酶還具有優先作用於單鏈DNA或RNA的3’→5’核酸外切酶（proofreading）活性。因此強烈推薦3′-modified的primers引物 Phi29 DNA Polymerase聚合酶應用 1.      Rolling circle amplification (RCA)：產生periodic DNA nanotemplates2.      多位移擴增Multiple displacement amplification（MDA）3.      Unbiased amplification無基因擴增全基因組 (WGA)4.      用於SNP和STR檢測擴增DNA5.      Protein-primed DNA擴增。

Description	Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.
Application	• Rolling circle amplification (RCA) • Multiple displacement amplification (MDA) • Whole genome amplification (WGA) • DNA template preparation for sequencing • Protein-primed DNA amplification
Form	Enzyme supplied with 5X Reaction Buffer
Concentration	10 U/μl
Storage	Store all components at -20°C.
Storage Buffer	50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol.
Notes	One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use .