Phi29 DNA Polymerase聚合酶 | 貨號M1239

A highly processive DNA polymerase which exhibits a strong strand-displacement function

產品描述：Phi29 DNA聚合酶是一種高度加工的polymerase聚合酶 （高達70 kb以上），具有很強的strand displacement activity鏈換置換活性。phi29 DNA聚合酶還具有優先作用於單鏈DNA或RNA的3’→5’核酸外切酶（proofreading）活性。因此強烈推薦3′-modified的primers引物

 

Phi29 DNA Polymerase聚合酶應用

–Rolling circle amplification (RCA)：產生periodic DNA nanotemplates

–多位移擴增Multiple displacement amplification（MDA)

–Unbiased amplification無基因擴增全基因組

–用於SNP和STR檢測擴增DNA

–從單細胞中cell-free amplification of DNA

–pathogenic organisms致病生物或metagenomes

–從filter paper blood spot samples濾紙血斑樣本中擴增DNA

–用於測序的DNA模板製備

–Protein-primed DNA擴增

–padlock probes掛鎖探針進行In situ genotyping原位基因分型

–Cell-free cloning of lethal DNA12.  RNA-primed DNA amplification