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Mouse Anti-Human IgE Fc-FITC抗體 | SouthernBiotech貨號9160-02

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Mouse Anti-Human IgE Fc-FITC抗體操作手冊

特點

Mouse Anti-Human IgE Fc-FITC抗體  | 貨號9160-02  |規格1.0 mL (0.5 mg/mL)

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全,包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質(FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)各種螢光標誌、另具酵素(HRP、AP)、或未標誌及Low Endotoxin/Azide Free(LE/AF)等標定。

Clone B3102E8
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-FITC
Specificity Human IgE Fc
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications

Applications for relevant formats of this clone include –

ELISA – Quality tested 1-3
FLISA – Quality tested
Flow Cytometry – Reported in literature 16
Immunohistochemistry-Frozen Sections – Reported in literature 4-13
Immunocytochemistry – Reported in literature 12
Western Blot – Reported in literature 2,3,12,14,15

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

References

  1. Xue L, Johnson R, Gorovits B. Prevalence and isotypic complexity of the anti-Chinese hamster ovary host cell protein antibodies in normal human serum. AAPS J. 2010;12:98-106. (ELISA)
  2. Goodman RE, Panda R, Ariyarathna H. Evaluation of endogenous allergens for the safety evaluation of genetically engineered food crops: review of potential risks, test methods, examples and relevance. J Agric Food Chem. 2013;61:8317-32. (ELISA, WB)
  3. Berkner H, Seutter von Loetzen C, Hartl M, Randow S, Gubesch M, Vogel L, et al. Enlarging the toolbox for allergen epitope definition with an allergen-type model protein. PLoS One. 2014;9(10):e111691. (ELISA, WB)
  4. Meding B, Ahonen K, Brisman J, Hedblad M, Ståhl A, Järvholm B, et al. Late skin-prick-test reactions to malted wheat. Clinical observations and immunohistochemical characterization. Allergy. 1998;53:282-8. (IHC-FS)
  5. Tengvall Linder M, Johansson C, Scheynius A, Wahlgren C. Positive atopy patch test reactions to Pityrosporum orbiculare in atopic dermatitis patients. Clin Exp Allergy. 2000;30:122-31. (IHC-FS)
  6. Åström K, Magnusson CG, Papatziamos G, Hemlin C, Scheynius A, van der Ploeg I, et al. Analysis of epsilon germline transcripts and IL-4 mRNA expression in the adenoids suggests local IgE switching. Allergy. 2000;55:1049-55. (IHC-FS)
  7. Sverremark Ekström E, Nilsson C, Holmlund U, van der Ploeg I, Sandstedt B, Lilja G, et al. IgE is expressed on, but not produced by, fetal cells in the human placenta irrespective of maternal atopy. Clin Exp Immunol. 2002;127:274-82. (IHC-FS)
  8. Nivenius E, Montan PG, Chryssanthou E, Jung K, van Hage-Hamsten M, van der Ploeg I. No apparent association between periocular and ocular microcolonization and the degree of inflammation in patients with atopic keratoconjunctivitis. Clin Exp Allergy. 2004;34:725-30. (IHC-FS)
  9. Rindsjö E, Varli IH, Ofori MF, Lundquist M, Holmlund U, Papadogiannakis N, et al. Presence of IgE+ cells in human placenta is independent of malaria infection or chorioamnionitis. Clin Exp Immunol. 2006;144:204-11. (IHC-FS)
  10. Forshammar J, Isaksson S, Strid H, Stotzer P, Sjövall H, Simrén M, et al. A pilot study of colonic B cell pattern in irritable bowel syndrome. Scand J Gastroenterol. 2008;43:1461-66. (IHC-FS)
  11. Spazierer D, Skvara H, Dawid M, Fallahi N, Gruber K, Rose K, et al. T helper 2 biased de novo immune response to Keyhole Limpet Hemocyanin in humans. Clin Exp Allergy. 2009;39:999-1008. (IHC-FS)
  12. Altrichter S, Kriehuber E, Moser J, Valenta R, Kopp T, Stingl G. Serum IgE autoantibodies target keratinocytes in patients with atopic dermatitis. J Invest Dermatol. 2008;128:2232-9. (IHC-FS, ICC, WB)
  13. Joerink M, Rindsjö E, Stenius F, Alm J, Lilja G, Grönlund H, et al. Evidence for allergen-specific IgE of maternal origin in human placenta. Allergy. 2009;64:905-12. (IHC-FS)
  14. Gómez-Ollés S, Cruz MJ, Renström A, Doekes G, Morell F, Rodrigo MJ. An amplified sandwich EIA for the measurement of soy aeroallergens. Clin Exp Allergy. 2006;36:1176-83. (WB)
  15. Venkatachalam M, Teuber SS, Peterson WR, Roux KH, Sathe SK. Antigenic stability of pecan [Carya illinoinensis (Wangenh.) K. Koch] proteins: effects of thermal treatments and in vitro digestion. J Agric Food Chem. 2006;54:1449-58. (WB)
  16. Buentke E, Zargari A, Heffler LC, Avila-Cariño J, Savolainen J, Scheynius A. Uptake of the yeast Malassezia furfur and its allergenic components by human immature CD1a+ dendritic cells. Clin Exp Allergy. 2000;30:1759-70. (FC)

 

 

 

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