Perfringens Agar (TSC) 產氣層瓊脂培養基 | 貨號NCM0077A/LAB194

Clostridium perfringens產氣莢膜梭狀芽孢桿菌培養

Perfringens Agar Base產氣莢膜瓊脂培養基是一種營養培養基，其中加入egg yolk emulsion蛋黃乳液（X073）/ (Egg Yolk Tellurite Emulsion (50 ml/100 ml per vial) HIMEDIA貨號FD046) 和cycloserine環絲氨酸（X194/NEOGEN NCM4022-0.5）用於製備胰蛋白腖亞硫酸鹽環絲氨酸（TSC）瓊脂。Sodium metabisulphite偏亞硫酸氫鈉和ferric ammonium citrate檸檬酸鐵銨用作Clostridium perfringens產氣莢膜梭菌（Clostridium perfringens）減少sulphite reduction亞硫酸鹽的指標。 通過C.perfringens減少亞硫酸鹽產生黑色菌落，並且摻入培養基中的蛋黃乳液檢測該細菌的卵磷脂酶活性。 然而，並非所有菌株都產生卵磷脂酶，因此黑色卵磷脂酶陽性和黑色卵磷脂酶陰性菌落應被視為推定的C.perfringens。

Minimum Q.C. organisms: Clostridium perfringens WDCM 00007 Escherichia coli WDCM 00013 (inhibition)

產氣層瓊脂培養基Base配方 Typical Formula g/litre

Tryptose——————————————————– 15.0

Soy Peptone —————————————————–5.0

Beef extract—————————————————– 5.0

Yeast extract—————————————————— 5.0

Sodium metabisulphite—————————————- 1.0

Ferric ammonium citrate—————————————- 1.0

Agar—————————————————————-14.0

 

培養基使用方法: 稱取41.0克粉末並分散在900ml去離子水中。 讓混合物浸泡10分鐘，旋轉混合併消毒；通過在121℃下高壓滅菌15分鐘。 讓培養基冷卻至45-50°C並補充2瓶X194（X194/NEOGEN NCM4022-0.5）和 100 mL of NCM4017* Egg Yolk Emulsion, 50%攪拌均勻，倒入無菌培養皿中。 省略蛋黃乳液用於製備無蛋黃。

1. Suspend 41 grams of the medium in 900 mL of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
4. Add 100 mL of NCM4017* Egg Yolk Emulsion, 50%, and the desired antimicrobic supplement: TSC Agar: Add 2 vials of NCM4022-0.5* TSC Perfringens Supplement, each reconstituted using 5 mL sterile deionized/RO water.

Overlay Agar

1. Suspend 41 grams of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
4. Overlay agar does not get supplemented with Egg Yolk Emulsion. Overlay agar is held at 45 -50°C until used and should be poured right after inoculation.

References

Shahidi, S.A. and Furguson, A.R. (1971). Appl. Microbiol. 21. 500-506.

Harmon, S.M., Kauttar, D.A. and Peeler, J.T. (1971). Appl. Microbiol. 22. 688-692.

Hauschild, A. H. W. and Hilsheimar R. (1973). Appl. Microbiol. 27. 78-82.

Hauschild A.H.W. and Hilsheimar, R. (1973). Appl. Microbiol. 27. 521-526.

Hauschild, A.H.W. et al (1977). Can. J. Microbiol. 23. 884-892.

Labbe, R. G. and Harmon, S.M. (1992). Compendium of methods for the microbiological examination of foods, 3rd ed 623-635.
TSC Perfringens Supplement產氣莢膜梭菌的選擇性培養基補充劑 | 貨號NCM4022-0.5 

Specifications
Brand	Neogen® Culture Media
Platform	Dehydrated Culture Media
Formula	Cycloserine – 400 mg/L
Preparation	No preparation required. Ready to use.
Previous Item Number	Product was previously known as Abtek product: #07-IS/CP3 Cycloserine for TSC Agar; and Lab M® product: #X194 D-Cycloserine Supplement
Storage Conditions	2-8°C
Volume	500
Quantity per Package	10
Package Weight	0.66 lb