人重组泛素醛 | 貨號4845-50

Ubiquitin Aldehyde, human recombinant-An inhibitor of deubiquitinylating enzymes (DUBs)

產品描述：Ubiquitin泛素的C-末端甘氨酸羧基被合成修飾成醛。蛋白醛（Ubiquitin Aldehyde , Ub-H）可用於體外穩定ubiquitin-protein conjugates綴合物，增強其在細胞裂解物和組織提取物。 Ub-H對去泛素化酶活性的抑制可用於鑑定和證實這種活性並確定特定酶之抑制動力學。泛素醛可用於活性分析實驗和DUB抑劑劑特異性測定。 它針對五個中的四個主要的DUB家族：UCH，USP，OUT和MJD，同時留下JAMM金屬蛋白酶不羈。 需要針對每種特定應用優化反應條件。 泛素醛與特定的去泛素化酶（模擬天然遍在蛋白底物的抑製劑）的Co-crystallization共結晶也已用於探測enzyme:substrate interactions。推薦初始重組人泛素醛濃度為1-5μM。

Cat # +Size	4845-50
Size	50 μg
Alternate Name	Ubiquitin Aldehyde
Source	E. coli
Appearance	Liquid
Physical Form Description	In 50 mM NaOAc pH 5.0
Molecular Weight	8.6 kDa
Purity by	>97% Pure active Recombinant human Ubiquitin Aldehyde.
Biological Activity	UCHL3 activity with Ubiquitin-AMC measured in the presence of 17.5 nM and 0.1 nM Ubiquitin Aldehyde.
Handling	Centrifuge the vial prior to opening.
Storage Conditions	-80°C
Shipping Conditions	Dry Ice