POD過氧化物酶活性檢測 | Peroxidase Activity Assay Kit貨號K772-100 (Abcam ab155895)

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POD過氧化物酶活性比色/螢光測定試劑盒 | 貨號K772-100 (Abcam ab155895)
Peroxidase Activity Colorimetric/Fluorometric Assay Kit

產品描述:Peroxidases過氧化物酶(EC編號1.11.1.x)是一大類酶,其通常催化形式的反應:ROOR’+電子供體(2e-)+ 2H +→ROH + R’OH。對於許多這些酶,最佳底物是hydrogen peroxide過氧化氫,但是其他酶對organic hydroperoxides有機氫過氧化物如lipid peroxides脂質過氧化物更具活性。Peroxidases過氧化物酶可在其活性位點含有heme cofactor血紅素輔因子,或者可選地含有氧化還原活性的cysteine半胱氨酸或selenocysteine residues硒代半胱氨酸殘基。 BioVision的Peroxidase Assay Kit過氧化物酶檢測試劑盒提供了一種方便的比色和螢光測定方法來測量生物樣品中的過氧化物酶活性。在過氧化物酶存在下,OxiRed探針以1:1的化學計量與H2O2反應,產生紅色螢光氧化產物-resorufin試鹵靈。通過比色(lmax = 570nm)或螢光測定法(Ex / Em = 535 / 587nm)量化, resorufin。該測定法簡單,直接,高靈敏度和高通量準備。根據我們的單位定義,通過比色法檢測限為每孔0.1 mU或通過螢光法檢測每孔0.01 mU。
| Cat # +Size | K772-100 |
| Size | 100 assays |
| Detection Method | Absorbance (570 nm) or Fluorescence (Ex/Em=535/587) |
| Species Reactivity | Mammalian |
| Applications | The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition. |
| Features & Benefits | • The assay is simple, direct, highly sensitive and high-throughput adaptable |
| Kit Components | • Assay Buffer • OxiRed™ Probe (in DMSO) • H₂O₂ Substrate (0.88 M) • HRP Positive Control |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
