附Protein marker 或 positive control

Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) 活性重組蛋白 |貨號APN576Hu61

產品介紹: Cloud clone提供原核及真核生物表現之重組蛋白系列商品；其中Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) 活性重組蛋白，為活性蛋白。純度> 97%，提供凍乾型式包裝。可應用於Cell culture; Activity Assays.等不同研究。

蛋白質保存型式

蛋白質溶於PBS, pH7.6, containing 5% trehalose.為冷凍乾燥包裝。使用前Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。

FNDC5活性重組蛋白相關之抗體 | 蛋白質 產品

 

The protein encoded by FNDC5 can be cleaved into Irisin, which is a myokine linked to exercise and lean body mass. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37oC.
Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.

Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of FNDC5 for 48h. The dose-effect curve of FNDC5 was shown in Figure 2. It was obvious that FNDC5 significantly decreased cell viability of MDA-MB-231 cells. The ED50 for this effect is typically 1.875 nM.

USAGE

Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Figure. Western Blot; Sample: Recombinant FNDC5, Human.

STORAGEAvoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.