Aldolase醛縮酶活性比色測定 | Aldolase Activity Colorimetric Assay Kit貨號K665-100 (abcam ab196994)

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Aldolase醛縮酶活性比色測定試劑盒 | 貨號K665-100 (abcam ab196994)
Aldolase Activity Colorimetric Assay Kit
檢測靈敏度醛縮酶活性小於0.1 mU (100 µU)
產品描述:醛縮酶(果糖二磷酸醛縮酶:EC 4.1.2.13)是糖酵解和糖異生的重要酶。它催化果糖-1,6-二磷酸與甘油醛-3-磷酸和二羥基丙酮的可逆反應。有2類Aldolase -I類:在動物和植物組織中發現,II類:在原核生物和低等真核生物中發現。 I型醛縮酶具有3種同工酶-A型:發現於肌肉和紅細胞中,B型:發現於肝臟和腎臟中,C型:發現於腦中。醛縮酶A缺乏導致肌病和溶血性貧血。肌肉疾病和肝損傷也可引起血清醛縮酶水平升高。精確檢測醛縮酶活性對於診斷和機制研究是有價值的。BioVision的醛縮酶活性測定中,醛縮酶將果糖-1,6-二磷酸轉化為甘油醛-3-磷酸和二羥基丙酮,並且通過一系列反應,將幾乎無色的探針還原為在450nm處具有吸光度的著色產物。該測定試劑盒簡單,靈敏,高通量適應性強。檢測靈敏度醛縮酶活性小於0.1 mU (100 µU)。

| Cat # +Size | K665-100 (abcam ab196994) |
| Size | 100 assays |
| Detection Method | Absorbance (OD 450 nm) |
| Applications | Measurement of Aldolase activity in various tissues/cells Analysis of glycolysis and gluconeogenesis pathways |
| Features & Benefits | • Rapid, convenient and high-throughput adaptable • Kit can detect aldolase activity less than 0.1 mU |
| Kit Components | • Aldolase Assay Buffer • Aldolase Substrate (Lyophilized) • Aldolase Enzyme Mix (Lyophilized) • Aldolase Developer (Lyophilized) • NADH Standard (Lyophilized) • Aldolase Positive Control (Lyophilized) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
