Human CellExp™ CD80, human recombinant重組蛋白 | 貨號7383

Plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages

產品描述：B7-1和B7-2以及它們的受體CD28和CTLA4構成調節T和B細胞應答的主要共刺激途徑之一。儘管CTLA4和CD28都可以結合相同的配體，但CTLA4以比CD28高20-100倍的親和力結合B71和B72，並參與免疫應答的下調。 B淋巴細胞活化抗原B7-1（也稱為B7）也稱為分化80（CD80）簇，是細胞表面免疫球蛋白超家族的成員，並在活化的B細胞，活化的T細胞，巨噬細胞和樹突細胞上表達。它是T細胞表面上兩種不同蛋白的配體：CD28（用於自體調節和細胞間結合）和CTLA-4（用於減弱調節和細胞解離）。 CD80與CD86一起起作用以引發T細胞。 CD80通過激活巨噬細胞中的NF-κB信號通路在誘導先天性免疫反應中起作用。 CD80因此被認為是自體免疫疾病和各種癌症的有希望的治療靶點。

Alternate Name	CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
Gene Symbol	CD80
Gene ID	941
Accession #	P33681
Source	HEK293 cells
Appearance	Lyophilized
Physical Form Description	Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight	This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 24.7 kDa. The predicted N-terminus is Val 35. DTT-reduced Protein migrates as 45-75 kDa due to different glycosylation.
Purity by SDS-PAGE	≥95%
Endotoxin Level	<1 EU/μg by LAL method
Biological Activity	Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.01 – 0.1 µg/mL in the presence of phytohaemagglutinin.
Reconstitution Instructions	Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.