Fc Silent™ Antibodies: Turning down the background noise 只要訊號不要雜訊 (Engineered Fc Domain)

Ab00458-1.1 Anti-dsRNA [9D5]

Absoluted Antibody 提供一系列抗體商品，其中Fc Silent™ Antibodies系列；能有效降低非特異性Fc結合。Fc Silent™技術，為應用生物工程技術，將Fc receptors (FcγR, FcR)點突變，進而中止抗體與ADCC的功能；有效降低Fc所造成的背景訊號。Fc Silent™抗體應用於免疫細胞時flow cytometry、immunohistochemistry (IHC)資料顯示，有效降低非特異性之Fc結合，所造成的背景值。(訂購抗體前，請確認選擇抗體是否有Fc Silent™修飾標誌)。

Anti-dsRNA [9D5] 單株抗體 | 貨號Ab00458-1.1

Species and Isotype: Mouse IgG1, kappa
Clone Number: 9D5
UniProt Accession Number of Target Protein: 
Alternative Name(s) of Target: double-stranded RNA; double-stranded ribonucleic acid
Published Application(s): ELISA; IHC; WB
Published Species Reactivity: Virus
Immunogen: RDV-RNA-methylated bovine serum albumin complex.
Specificity: 
Application Notes: The antibody binds specifically to ds-RNA, which is part of the genetic material of some viruses, such as rotaviruses and the bluetongue virus. The antibody can be used as a diagnostic tool, to determine the nature of an unknown pathogen, and could potentially be used in gene therapy.
Antibody First Published in: 
Kitagawa et al. Immunogenicity of rice dwarf virus-ribonucleic acid. Tohoku J. Exp. Med. 122 (4), 337-343 (1977) PMID:918970 Note on publication: Describes the production and characterisation of antibodies against double-stranded RNA found in the rice dwarf virus (RDV). the antibodies are produced in rabbits immunized with anti-RDV-RNA-methylated bovine serum albumin complexes.

Immunofluorescence staining of cells infected with a Dengue 2 virus using anti-dsRNA antibody (Ab00458) 9D5. Immunofluorescence analysis of Triton-permeabilised paraformaldehyde-fixed cells stained with the chimeric rabbit version of 9D5 (Ab00458-23.0) at 3.3 µg/ml followed by Alexa Fluor® 568 secondary antibody, showing dsRNA staining. The nuclear stain is DAPI (blue). Images are kind courtesy of Cecilia Alejandra Vazquez (IQUIBICEN (UBA-CONICET)).