Abmgood不朽化系列商品 (immortalized cell)

永生化的人類冠狀動脈平滑肌細胞 Immortalized Human Coronary Artery Smooth Muscle Cells | (貨號T0557)

Organism	Homo sapiens
Source Organ	Coronary Arteries
BioSafety Level	II
Growth Properties	Adherent
Morphology	Multipolar
Recommended Seeding Density	5000 cells/cm2
Population Doubling	35-40hours
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 whole gene
Applications	For Research Use Only
Markers	Smooth Muscle Actin

永生化的人類冠狀動脈平滑肌細胞 細胞特性: Adherent型態Multipolar來源Coronary Arteries。細胞培養條件: 使用PriCoat™T25培養瓶 (貨號G299) 以最佳化初代和不朽細胞的生長、增殖和擴增。或塗層細胞外基質(Applied Cell Extracellular Matrix) 增強細胞貼附 (貨號G422)。Grow cells in ECM-coated culture vessels with the following conditions. 培養液PriGrowII (貨號 TM002)，胎牛血清(Cat. No. TM999)最終濃度為10%並填加1% Penicillin/Streptomycin 。1%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C

產品資料請按這PriCoat™ T25 Flasks | type I collagen coating | I 型膠原(type I collagen) | Applied Cell Extracellular Matrix | Prigrow III Medium初代細胞培養液

HOT!! 黴漿菌PCR偵測 | DAPI染核 | 細胞內黴漿菌去除劑 | 水浴槽抑菌劑 | 噴霧式去黴劑 等系列商品

Abmgood不朽化系列商品 (immortalized reagenet) Ready-to-use

Abmgood公司於人類與老鼠不朽之細胞株上，具有幾十年的實驗經驗；提供一系列不朽 (immortalized)化之細胞株、及不朽化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus，可直接加於細胞中進行感染表現；病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液；亦提供一系列病毒力價濃縮與定量試劑商品。

細胞不朽化的工具中，SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因（p53、Rb等）的不活化相關，會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞，SV40 T anbtigen會誘導端粒酶（Telomerase）的活性。與端粒（telomere）長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中，hTERT是處於不活化態，必要時會進行活化保持telomere的長度，避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現（over-expression）對於誘發細胞不朽化是無效的（如初代上皮細胞），反而會造成細胞死亡；同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。

Product	Size	Cat. No.
Immortalized Human Carotid Artery Smooth Muscle Cells – hTERT	1×106 cells / 1.0 ml	T0511
Immortalized Human Carotid Artery Smooth Muscle Cells – SV40	1×106 cells / 1.0 ml	T0510
Immortalized Human Brachiocephalic Artery Endothelial Cells – SV40	1×106 cells / 1.0 ml	T0513
Immortalized Human Carotid Artery Endothelial Cells – SV40	1×106 cells / 1.0 ml	T0512
Immortalized Human Cardiac Microvascular Endothelial Cells – SV40	1×106 cells / 1.0 ml	T0514
Immortalized Human Aortic Endothelial Cells- SV40	1×106 cells / 1.0 ml	T0447
Immortalized Human Pulmonary Artery Endothelial Cells	1×106 cells / 1.0 ml	T0550
Immortalized Human Coronary Artery Endothelial Cells	1×106 cells / 1.0 ml	T0551
Immortalized Human Internal Thoracic Artery Endothelial Cells	1×106 cells / 1.0 ml	T0552
Immortalized Human Subclavian Artery Endothelial Cells	1×106 cells / 1.0 ml	T0553
Immortalized Human Iliac Artery Endothelial Cells	1×106 cells / 1.0 ml	T0555
Immortalized Human Subclavian Artery Smooth Muscle Cells	1×106 cells / 1.0 ml	T0556
Immortalized Human Coronary Artery Smooth Muscle Cells	1×106 cells / 1.0 ml	T0557
Immortalized Human Pulmonary Artery Smooth Muscle Cells	1×106 cells / 1.0 ml	T0558
Immortalized Human Internal Thoracic Artery Smooth Muscle Cells	1×106 cells / 1.0 ml	T0560
Immortalized Human Brachiocephalic Artery Smooth Muscle Cells	1×106 cells / 1.0 ml	T0561
Immortalized Human Aortic Smooth Muscle Cells – SV40	1×106 cells / 1.0 ml	T0515
Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells	1×106 cells/ml	T0549