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乳糖比色/螢光測定試劑盒 | 貨號K624   (ABCAMab83384)

Lactose Colorimetric/Fluorometric Assay Kit

產品描述：乳糖（Lactose, C 12 H 22 O 11 FW：342.3）是一種重要的天然存在的二糖 (disaccharide)，由一個半乳糖(galactose)和一個葡萄糖(glucose)組成。 牛奶(Milk)含有2-8％的乳糖(lactose)。 有些人，尤其是嬰兒( infants)，缺乏消化半乳糖(galactose)所需的酶，導致血液中半乳糖的積累（Galactosemia半乳糖血症），引起肝臟腫大(enlarged liver)，腎功能衰竭(cataracts)，白內障(cataracts )和腦損傷(brain damage)。 在BioVision乳糖分析試劑盒 (BioVision Lactose Assay Kit, K624)中，乳糖被水解成葡萄糖( glucose )和半乳糖(galactose)。 隨後將半乳糖氧化產生顏色（O.D 570nm）和螢光（Ex / Em 535 / 587nm)。在沒有乳糖酶的情況下，游離半乳糖可以通過背景控制進行校正。 乳糖測定試劑盒為各種生物樣品（血清，血漿，其他體液，食物，生長介質等）中乳糖水平的直接測量，提供了簡單，方便和靈敏的方法。此產品 ( 570nm呈色分析) 需由儀器進行定量 ELISA儀器設備請按此。螢光分析（Ex / Em 535 / 587nm) 請用螢光reader儀器檢測。

此商品可以使用乳製品樣品； 由於它們尚未經過專門測試，建議測試幾個劑量的樣品，以確保讀數在標準曲線線性範圍內。 確保樣品不渾濁也很重要，因為這會影響吸光度測量；另確認 游離半乳糖是否干擾測定。 乳製品中存在半乳糖，建議為每個樣品準備兩個Well。 向一個well中加入2ul乳糖酶 (Lactase )，另一個Well中加入2ul assay buffer 用於半乳糖背景對照。 可以從乳糖分析中減去半乳糖背景。若需要測量半乳糖及乳糖；可使用K617 (Galactose and Lactose Colorimetric/Fluorometric Assay Kit) ; K617不是直接測量乳糖。 總游離半乳糖和游離半乳糖之間的差異計算為K617中的乳糖濃度。 這樣做是為了能夠測量同一樣品中的半乳糖和乳糖。

• Product name

  Lactose Assay Kit

• Detection method

  Colorimetric/Fluorometric

• Sample type

  Milk, Other biological fluids

• Assay type

  Quantitative

• Assay time

  1h 00m

• Product overview

  In Abcam’s Lactose Assay Kit Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.
  Visit our FAQs page for tips and troubleshooting.

• Notes

  This product is manufactured by BioVision, an Abcam company and was previously called K624 Lactose Colorimetric/Fluorometric Assay Kit. K624-100 is the same size as the 100 test size of ab83384.

  Lactose (C₁₂H₂₂O₁₁ FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

• Platform

  Microplate reader

引用文獻

 L L Hernandez, et. el. Suppression of lactation and acceleration of involution in the bovine mammary gland by a selective serotonin reuptake inhibitor. J. Endocrinol., Apr 2011; 209: 45 – 54.