IL17RB siRNA Oligos set (Human) | 貨號   i510917

siRNA是用於基因沉默的強大工具，可以通過多種方法遞送至靶細胞或宿主，包括腺病毒，慢病毒或直接轉染DNA質粒或雙鏈RNA（dsRNA）。 siRNA oligo雙鏈體是快速和容易使用的，並且可以在體外和體內用於快速和短暫的基因沉默而不改變宿主基因組。For stable knockdown via lentivirus, please see our lentiviral siRNA library products.abm提供了針對任何人，小鼠或大鼠感興趣的基因的預先設計的3種siRNA寡核苷酸雙鏈體，以及客戶實驗的陽性和陰性對照。雙鏈體可以單獨轉染或合併在一起以實現目標基因的knockdown，最常見的是通過qPCR或western印跡進行評估。提供之siRNA寡核苷酸是化學修飾的（2′-OMe），無需增加額外的電荷，從而提高體外和體內的穩定性和增強的knockdown作用，預pre-annealed,及 lyophilized powder凍乾粉末運輸，以便更長時間和更穩定的儲存。

SiRNA Oligos產品特點

1. HPLC純化：siRNA含量> 97％
2. 設置3個siRNA寡核苷酸雙鏈體，最大限度地maximum knockdown
3. 每個siRNA寡核苷酸雙鏈體為19-23個鹼基
4. 化學修飾（2′-OMe）用於增加體外和體內的穩定性，具更高的功效和更低的毒性
5. 附正面和負面對照
6. 免費設計; 保證knockdown至少70％

Species	Human
Accession Number	NM_018725
Components Included	3 x 5nmol (2 OD duplex, 1 OD single) of chemically modified siRNA oligos (3 target sequences) 1 x 2.5nmol (1 OD duplex, 0.5 OD single) of positive control siRNA 1 x 2.5nmol (1 OD duplex, 0.5 OD single) of negative control siRNA 1 x 2.5nmol (1 OD duplex, 0.5 OD single) of FAM-labeled negative control siRNA
Conversion of ODs, nmols and μg	1 OD = 2.5 nmol siRNA (duplex) = 5 nmol siRNA (single-stranded) = 33 μg
Additional Reagents Required (not supplied)	RNAifectin(Cat.No.G073)
Target Sequence	Sequence available upon placing order
Chemical Modifiation	2′-OMe modification for increased stability and low cell toxicity
Format	Lyophilized double stranded siRNA oligos
Purification	HPLC
Storage	Upon receipt, -70°C for long term storage; -20°C for short term storage. Recommended storage concentration is above 20μM. After reconstitution, store in aliquots to avoid repeated freeze-thawing.
Shelf Life	Up to 6 months if stored at -20°C or below after reconstitution in a non-frost free freezer. 1 year lyophilized (when at -20°C or below in a non-frost free freezer)