Caspase-3胱天蛋白酶-3免疫活性測定試劑盒 | 貨號K163-100

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Caspase-3胱天蛋白酶-3免疫活性測定試劑盒 | 貨號K163-100
CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)
Specifically detects Caspase-3
產品描述:Caspase-3胱天蛋白酶-3的激活在細胞凋亡過程中,扮演關鍵作用。 CaspSELECT™Caspase-3免疫測定試劑盒提供了一種有效的免疫吸附測定法,於微量滴定板中檢測caspase-3活性的特異性。定量該測定法利用Anti-Caspase-3 Antibody胱天蛋白酶-3多克隆抗體從細胞裂解物中捕獲活化的Caspase-3半胱天冬酶-3。然後加入半胱天冬酶底物DEVD-AFC,並與細胞裂解物中活化的半胱天冬酶-3的量成比例地切割。 裂解產生Free的AFC,其可以使用螢光板讀數器進行螢光測定(Ex./Em= 400nm / 505nm)分析。 該測定確保半胱天冬酶-3的絕對特異性檢測。
Cat # +Size | K163-100 |
Size | 100 assays |
Detection Method | Fluorescence (Ex/Em 370-425/ 490-525 nm) |
Species Reactivity | Mammalian |
Applications | Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. |
Features & Benefits | • Simple one-step procedure; takes only ~3 hours • Fast and convenient |
Kit Components | • Cell Lysis Buffer • Coating Buffer • Anti-Caspase-3 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1 M) • DEVD-AFC Substrate (1 mM) • Positive Control (rh-Caspase-3) • Microtiter Plate • Adhesive Plate Cover |