Polybrene | 貨號 G062 (1.0 ml; 0.8 mg/ml)

Polybrene is diluted in purified, sterile filtered water, at a concentration of 0.8 mg/mL

聚凝胺(Polybrene)，或稱為hexadimethrine bromide。一種cationic polymer陽離子聚合物，常用於哺乳動物細胞之DNA感染實驗，可增強脂質體的感染效率。廣泛用於反轉錄病毒介導的基因感染retrovirus (cell culture)，慢病毒介導的基因感染，作用機制可能是通過neutralizing the charge repulsion中和細胞表面唾液酸與病毒顆粒之間的靜電排斥從而促進吸附作用。其也是一種有名的抗肝素劑（肝素拮抗劑），常用來生產非特異性凝集的紅細胞。另外也多用於蛋白測序(protein sequencing)，因為小劑量的聚凝胺在自動測序分析可明顯改善多胜肽的降解現象。Polybrene A highly efficient method of gene transfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene is diluted in purified, sterile filtered water, at a concentration of 0.8 mg/mL

Description	As a cationic polymer, polybrene increases the efficiency of infection in some cells with retrovirus (cell culture) by neutralizing the charge repulsion between virions and the cell surface. It can also be utilized in protein sequencing.
Storage	Store at -20°C.
Reference	Ordelheide, A. M., et al (2013). Nor-1, a novel incretin-responsive regulator of insulin genes and insulin secretion. Molecular Metabolism (2):243-255.

Desmarets, LM et al. “Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses” Vet Res. 44:71 (2013). DOI: 10.1186/1297-9716-44-71. Application: Lentivirus transduction.
Olyslaegers, DA et al. “Generation and characterization of feline arterial and venous endothelial cell lines for the study of the vascular endothelium” BMC Vet Res. 9:170 (2013). DOI: 10.1186/1746-6148-9-170. Application: Lentivirus transduction.
Ordelheide, AM et al. “Nor-1, a novel incretin-responsive regulator of insulin genes and insulin secretion” Mol Metab. 2 (3):243-255 (2013). DOI: 10.1016/j.molmet.2013.06.003. Application: Lentivirus transduction.
Nauwynck, H.J. et al. “Generation and Characterization of feline arterial and cenous endotheilial cell lines for the study of the vascular endothelium ” Biomed Central 9: (2013). DOI: 10.1186/1746-6148-9-170. Application: Gene Delivery.
Laval, K et al. “Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells” J Virol 21:10912-10923 (2015). DOI: 10.1128/JVI.01589-15. PubMed: 26292328. Application: Lentivirus transduction.