Shanghai Crystal Day Biotech成立於2009年，前身為Shanghai Korain Biotech，與大學實驗室合作，旨在為實驗室研究人員提供優質、實惠的ELISA工具。 經過五年的研究和改進，Shanghai Korain Biotech成為ELISA試劑盒的專有平台，並創立了“bt-laboratory（簡稱BT LAB）

Rotavirus antigen ELISA試劑盒 | Human Rotavirus antigen, RV Ag ELISA Kit貨號ED0076Hu

產品描述：Rotavirus antigen ELISA試劑盒, 用於定性(Qualitative)樣品中的Rotavirus antigen（RV Ag）。測量物種: Human。該試劑盒基於定性反相酶免疫分析技術(reverse phase enzyme immuno assay technique)。 微量滴定板已預先塗有目標抗體。 將陽性/陰性對照或樣品加入孔中並孵育。 樣品中的抗原與板上的抗體結合。 未結合的抗原在洗滌步驟中被洗掉。 然後加入辣根過氧化物酶 (Horseradish Peroxidase , HRP) 偶聯的檢測抗體並孵育。 未結合的 HRP 在洗滌步驟中被洗掉。 然後加入 TMB 底物並顯色。 通過加入酸性終止溶液終止反應，顏色變為黃色，可在 450 nm 處測量。 然後可以將未知樣品的 OD 與陽性和陰性對照的 OD 進行比較，以確定 RV Ag 的存在。可應用於以下樣品來源: Serum, Plasma, Cell culture supernatant

Product Summary

Size: 96 Test

Assay type: Qualitative

Sample type: Serum, Plasma, Cell culture supernatant

Reactive with: Human

Shanghai Crystal Day Biotech成立於2009年，前身為Shanghai Korain Biotech，與大學實驗室合作，旨在為實驗室研究人員提供優質、實惠的ELISA工具。 經過五年的研究和改進，Shanghai Korain Biotech成為ELISA試劑盒的專有平台，並創立了“bt-laboratory（簡稱BT LAB）”品牌，擁有6, 000+ ELISA試劑盒的豐富產品線 包括夾心ELISA（酶聯免疫吸附測定）試劑盒、Competitive EIA（酶免疫測定）試劑盒。 2018 年，BT LAB 推出了擁有 10,000+ 多種抗體的抗體系列及10,000+ ELISA套組。2019年10月，我們的實驗室和倉庫在浙江嘉興建成。 現在 Korain 在 5 大洲的 35 個國家/地區發展壯大，引用次數超過 6,000+。

酶聯免疫吸附試驗 (enzyme-linked immunosorbent assay , ELISA) 是一種常用的分析生化分析方法(biochemistry assay)。 這測定使用固相類型的酶免疫測定(enzyme immunoassay)檢測液體樣品中蛋白質的存在使用針對蛋白質的抗體測量。 在最簡單的 ELISA 形式中，抗原從樣品（如血清、血漿、細胞培養上清液、細胞裂解液、唾液、組織裂解液和尿液）被測試的物體附著在一個表面上。 然後，一個匹配的抗體被塗在表面上，所以它可以結合抗原。 這種抗體與酶相連然後去除任何未結合的抗體。 在最後一步，含有酶的物質添加底物。 如果有綁定後續反應產生可檢測的信號，最常見的是顏色變化。

High sensitivity and specificity ELISA kit Sensitivity of the assay is the smallest detectable amount of the target protein in question, it depends mainly on the affinity of the solid phase antibody according to the law of mass action. Specificity helps us recognize if the antibody detect the target protein specifically without crossreacting with non-pecific proteins. Monoclonal antibodies have very high affinity for the antigen, which also have higher specificity than polyclonal antibodies. A monoclonal antibody is used as the detecting antibody in BT LAB sandwich ELISA to provide improved specificity. • Extensive analyte selection • Strict QC control • Vadidated sample testing

高靈敏度(sensitivity)和特異性(specificity )ELISA試劑盒

測定的靈敏度(sensitivity)是所討論的目標蛋白質的最小可檢測量，它根據質量作用定律，主要取決於固相抗體的親和力。特異性幫助我們識別抗體是否特異性檢測目標蛋白質，而不會與非特異性蛋白質發生交叉反應。 單克隆抗體對抗原有很高的親和力，其特異性也高於多克隆抗體。 單克隆抗體用作BT LAB 夾心 ELISA 中的檢測抗體可提供更高的特異性。

• 廣泛的分析物選擇
• 嚴格的質量控制
• 驗證樣本測試

精度和再現性Precision & Reproducibility

精密度(Precision)是作為平均值的變異係數 (variation , CV) 來衡量的。

CV 應根據計算出的濃度而不是原始光學值來計算密度。

• 小於15 的分析間% CV 通常是可以接受的。
• 檢測內 % CV 應小於 10。
• 報告反映結果範圍的濃度的 CV 很重要標本中發現。

BT Lab ELISA Types There are several ELISA configurations, with the most common formats being sandwich, competitive and signaling assays. Concentration-dependent detection of the target analyte is accompanied by either an increase or a decrease in signal, which is related to the format that is chosen.

E0000Mk	Monkey	Monkey Hyaluronic acid,HA ELISA Kit	96 wells/48 wells
E0001Mk	Monkey	Monkey Apolipoprotein C3,Apo C3 ELISA Kit	96 wells/48 wells
E0002MK	Monkey	Monkey Prostaglandin E2,PG-E2 ELISA Kit	96 wells/48 wells
E0004MK	Monkey	Monkey Hemoglobin subunit beta,HBB ELISA Kit	96 wells/48 wells
E0005MK	Monkey	Monkey transforming growth factor α,TGF-α ELISA Kit	96 wells/48 wells
E0007MK	Monkey	Monkey Lymphotoxin-beta,LTB ELISA Kit	96 wells/48 wells
E0009MK	Monkey	Monkey adiponectin,ADP ELISA Kit	96 wells/48 wells
E0011MK	Monkey	Monkey Glutathione reductase,GR ELISA Kit	96 wells/48 wells
E0012MK	Monkey	Monkey secretory immunoglobulin A,SIgA ELISA Kit	96 wells/48 wells
E0013MK	Monkey	Monkey Microalbunminuria,MAU/ALB ELISA Kit	96 wells/48 wells
E0014MK	Monkey	Monkey TEK tyrosine kinase,Endothelial,Tie2 ELISA Kit	96 wells/48 wells
E0015MK	Monkey	Monkey Interleukin 17,IL-17 ELISA Kit	96 wells/48 wells
E0016MK	Monkey	Monkey Prolactin,PRL ELISA Kit	96 wells/48 wells
E0017MK	Monkey	Monkey mast cell tryptase,MCT ELISA Kit	96 wells/48 wells
E0018MK	Monkey	Monkey Dihydrotestosterone,DHT ELISA Kit	96 wells/48 wells
E0019MK	Monkey	Monkey Insulin,INS ELISA Kit	96 wells/48 wells
E0020MK	Monkey	Monkey Histamine,HIS ELISA Kit	96 wells/48 wells
E0021MK	Monkey	Monkey Complement fragment 5a,C5a ELISA Kit	96 wells/48 wells
E0022MK	Monkey	Monkey Complement fragment 3a,C3a ELISA Kit	96 wells/48 wells
E0024MK	Monkey	Monkey S100 calcium binding protein A4,S100A4 ELISA Kit	96 wells/48 wells
E0025MK	Monkey	Monkey matrix metalloproteinase,MMP-7 ELISA Kit	96 wells/48 wells
E0026MK	Monkey	Monkey neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit	96 wells/48 wells
E0027MK	Monkey	Monkey tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit	96 wells/48 wells
E0029MK	Monkey	Monkey islet cell antibody,ICA ELISA Kit	96 wells/48 wells
E0030MK	Monkey	Monkey C-Peptide,C-P ELISA Kit	96 wells/48 wells
E0031MK	Monkey	Monkey Interleukin 1α,IL-1α ELISA Kit	96 wells/48 wells
E0032MK	Monkey	Monkey Interleukin 4,IL-4 ELISA Kit	96 wells/48 wells
E0033MK	Monkey	Monkey Interleukin 5,IL-5 ELISA Kit	96 wells/48 wells
E0034MK	Monkey	Monkey Interleukin 6,IL-6 ELISA Kit	96 wells/48 wells
E0035MK	Monkey	Monkey Interleukin 10,IL-10 ELISA Kit	96 wells/48 wells
E0036MK	Monkey	Monkey Transforming Growth factor β1,TGF-β1 ELISA Kit	96 wells/48 wells
E0037MK	Monkey	Monkey Transforming Growth factor β2,TGF-β2 ELISA Kit	96 wells/48 wells
E0038MK	Monkey	Monkey Interleukin 1β,IL-1β ELISA Kit	96 wells/48 wells
E0039MK	Monkey	Monkey C-reactive protein,CRP/PTX1 ELISA Kit	96 wells/48 wells
E0040MK	Monkey	Monkey Serum Amyloid A2,SAA2 ELISA Kit	96 wells/48 wells
E0041MK	Monkey	Monkey Regenerating Islet Derived Protein 3 Alpha,REG3a ELISA Kit	96 wells/48 wells
E0042MK	Monkey	Monkey Hemoglobin,HB ELISA Kit	96 wells/48 wells
E0043MK	Monkey	Monkey cytochrome P450,CYP450 ELISA Kit	96 wells/48 wells
E0044MK	Monkey	Monkey Cytochrome P450 3A4,CYP3A4 ELISA Kit	96 wells/48 wells
E0046MK	Monkey	Monkey Progesterone,Prog ELISA Kit	96 wells/48 wells
E0047MK	Monkey	Monkey Myxovirus Resistance 1,MX1 ELISA Kit	96 wells/48 wells
E0049MK	Monkey	Monkey Adrenocorticotropic Hormone,ACTH ELISA Kit	96 wells/48 wells
E0050MK	Monkey	Monkey Serotonin,ST ELISA Kit	96 wells/48 wells
E0051MK	Monkey	Monkey Interferon γ ,IFN-γ ELISA Kit	96 wells/48 wells
E0052MK	Monkey	Monkey Luteinizing Hormone,LH ELISA Kit	96 wells/48 wells
E0053MK	Monkey	Monkey Fructosamine,FTA ELISA Kit	96 wells/48 wells
E0087MK	Monkey	Monkey Malondialdehyde ELISA KIT	96 wells/48 wells
E0088MK	Monkey	Monkey Tumor necrosis factor α,TNF-α ELISA Kit	96 wells/48 wells
E0090MK	Monkey	Monkey Substance P ELISA kit	96 wells/48 wells
E0091MK	Monkey	Monkey Hepatitis A virus cellular receptor 1 homolog,HAVCR1/KIM-1 ELISA KIT	96 wells/48 wells
E0092MK	Monkey	Monkey D-Dimer,D2D ELISA Kit	96 wells/48 wells
E0093MK	Monkey	Monkey amyloid beta peptide 1-42,Aβ1-42 ELISA Kit	96 wells/48 wells
E0094MK	Monkey	Monkey Beta-defensin 1,DEFB1 ELISA kit	96 wells/48 wells
E0095MK	Monkey	Monkey Beta-defensin 123,DEFB123 ELISA kit	96 wells/48 wells
E0096MK	Monkey	Monkey Beta-microseminoprotein,MSMB ELISA kit	96 wells/48 wells
E0097MK	Monkey	Monkey brain natriuretic peptide,BNP ELISA Kit	96 wells/48 wells
E0098MK	Monkey	Monkey Brain-derived neurotrophic factor,BDNF ELISA kit	96 wells/48 wells
E0099MK	Monkey	Monkey Cathelicidin antimicrobial peptide,CAMP ELISA kit	96 wells/48 wells
E0100MK	Monkey	Monkey C-C motif chemokine 17,CCL17 ELISA kit	96 wells/48 wells
E0101MK	Monkey	Monkey Chymotrypsin-C,CTRC ELISA kit	96 wells/48 wells
E0102MK	Monkey	Monkey circulating immune complex,CIC ELISA Kit	96 wells/48 wells
E0103MK	Monkey	Monkey Complement 3,C3 ELISA Kit	96 wells/48 wells
E0104MK	Monkey	Monkey Complement 4,C4 ELISA kit	96 wells/48 wells
E0106MK	Monkey	Monkey C-X-C motif chemokine 10,CXCL10 ELISA kit	96 wells/48 wells
E0107MK	Monkey	Monkey Cysteine-rich secretory protein 1,CRISP1 ELISA kit	96 wells/48 wells
E0108MK	Monkey	Monkey Cytochrome P450 1A1,CYP1A1 ELISA kit	96 wells/48 wells
E0109MK	Monkey	Monkey Endothelin 1,ET-1 ELISA Kit	96 wells/48 wells
E0110MK	Monkey	Monkey Erythropoietin,EPO ELISA kit	96 wells/48 wells
E0111MK	Monkey	Monkey gastrin,GAST ELISA kit	96 wells/48 wells
E0112MK	Monkey	Monkey Glutathione S-transferase P,GSTP1 ELISA kit	96 wells/48 wells
E0113MK	Monkey	Monkey Growth hormone receptor,GHR ELISA kit	96 wells/48 wells
E0115MK	Monkey	Monkey IgG receptor FcRn large subunit p51,FCGRT ELISA kit	96 wells/48 wells
E0116MK	Monkey	Monkey immunoglobulin A,IgA ELISA kit	96 wells/48 wells
E0117MK	Monkey	Monkey Immunoglobulin M,IgM ELISA kit	96 wells/48 wells
E0118MK	Monkey	Monkey Insulin-like Growth Factor 1,IGF-1 ELISA KIT	96 wells/48 wells
E0119MK	Monkey	Monkey Interferon α,IFN-α ELISA Kit	96 wells/48 wells
E0120MK	Monkey	Monkey Interferon β,IFN-β ELISA Kit	96 wells/48 wells
E0121MK	Monkey	Monkey interleukin 16,lymphocyte chemoattractant factor,IL16 ELISA kit	96 wells/48 wells
E0122MK	Monkey	Monkey Interleukin-2 receptor,IL-2R ELISA kit	96 wells/48 wells
E0123MK	Monkey	Monkey Interleukin-8,IL-8 ELISA Kit	96 wells/48 wells
E0124MK	Monkey	Monkey macrophage colony-stimulating factor,M-CSF ELISA kit	96 wells/48 wells
E0125MK	Monkey	Monkey Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit	96 wells/48 wells
E0126MK	Monkey	Monkey Macrophage Inflammatory Protein-3α,MIP-3α/CCL20 ELISA Kit	96 wells/48 wells
E0127MK	Monkey	Monkey Macrophage Migration Inhibitory Factor,MIF ELISA Kit	96 wells/48 wells
E0128MK	Monkey	Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit	96 wells/48 wells
E0129MK	Monkey	Monkey motilin,MLN ELISA kit	96 wells/48 wells
E0130MK	Monkey	Monkey N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT	96 wells/48 wells
E0131MK	Monkey	Monkey Osteocalcin,BGLAP ELISA kit	96 wells/48 wells
E0132MK	Monkey	Monkey Proprotein convertase subtilisin/kexin type 9,PCSK9 ELISA kit	96 wells/48 wells
E0133MK	Monkey	Monkey secreted frizzled-related protein 1,SFRP1 ELISA kit	96 wells/48 wells
E0134MK	Monkey	Monkey Somatotropin,GH1 ELISA kit	96 wells/48 wells
E0135MK	Monkey	Monkey thyroid stimulating hormone,TSH ELISA kit	96 wells/48 wells
E0136MK	Monkey	Monkey Tissue factor pathway inhibitor,TFPI ELISA kit	96 wells/48 wells
E0137MK	Monkey	Monkey Toll-like receptor 2,TLR2 ELISA kit	96 wells/48 wells
E0138MK	Monkey	Monkey Vascular Endothelial cell Growth Factor,VEGF ELISA Kit	96 wells/48 wells
E0139MK	Monkey	Monkey Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit	96 wells/48 wells
E0140MK	Monkey	Monkey Stromal cell derived factor 1,SDF-1 ELISA Kit	96 wells/48 wells
E0141MK	Monkey	Monkey Nuclear Factor Kappa B,NFkB ELISA Kit	96 wells/48 wells
E0142MK	Monkey	Monkey Interleukin 12,IL-12 ELISA Kit	96 wells/48 wells
E1374MK	Monkey	Monkey Carboxymethyl Lysine,CML ELISA kit	96 wells/48 wells