InnovaCoat® GOLD 80nm奈米金抗體標誌產品

廠牌INNOVA BIOSCIENCES 貨號231-0010 (10 reactions) 規格10個反應
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傳統奈米金 (passive coating methods using naked gold) 抗體標誌，受限於等電點、pH、salt等問題。因此，奈米金標誌效能不高，穩定性不好，且會有失效等問題。InnovaCoat專利研發奈米金標誌，應用共價鍵結，將抗體/蛋白質/生物分子鍵結於奈米金上，其效能近乎100%，穩定性極佳。且不須考量等電點及pH之問題，操件僅需2分鐘。應用於lateral flow test快篩試驗的開發, 其靈敏度高於ELISA方式。同時廣泛應用於lateral flow test、電鏡、免疫、分子生物學以至生物晶片中。實驗證明，該法樣本用量少、檢測速度快、對比明顯、操作簡單、敏感性和特異性高，既可用於抗原檢測，也可用於抗體檢測診斷。

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InnovaCoat® GOLD – 20OD 80nm Gold Particle Kits

訂購貨號#231-0010 (10個反應)

訂購貨號#231-0005 (3個反應)

奈米金抗體標誌/特點

1. 抗體標誌效能近乎100%, 節省抗體用量。每次反應僅需要0.4ug~0.17ug抗體量 (濃度50ug/1000ul~200ug/1000ul)。

2. 一次反應, 可進行50次的lateral flow test。(體積為50ul, 可以稀釋為1ml)

3. 一組共有10個反應, 每個反應可進行50次lateral flow test。

4. 抗體標誌完奈米金後, 其訊號高於傳統奈米金標誌。

5. 不須考量等電點及pH問題。

6. 奈米標誌僅需要15分鐘。

7. 建立良好的奈金標誌抗體, 其靈敏度遠高於ELISA方法

8. 傳統市售奈米金標誌, 其O.D為0.5,
InnovaCoat的光密度為O.D=20 金球的產品(InnovaCoat? GOLD – 20OD 40nm Gold Particle Kits and InnovaCoat GOLD? Streptavidin)濃度分別是20 OD和10 OD，1個O.D為9.0 x 10¹⁰ particles/mL

9. 奈米金辨視NH3端, 以共價鍵鍵結, 性質穩定。

奈米金抗體標誌/產品系列

InnovaCoat™ GOLD – 20OD 40nm Gold Particle
Kits, 230-0010

InnovaCoat™
GOLD products are ‘conjugation friendly’ nanoparticles with a proprietary surface coat that greatly enhances colloidal stability and permits easy covalent attachment of a variety of molecules, including antibodies, analytes and other biomolecules. In thecase of immuno-gold conjugates, the antibody can be attached irreversibly without the need for extensive trials at different values of pH and/or salt concentration, as is typical of traditional ‘passive’ binding methods.

In the case of analyte-gold conjugates, the multipoint attachment of the polymer to the gold surface ensures that the analyte is firmly anchored. By contrast, direct attachment of analytes to gold surfaces by self-assembly techniques is far less stable and unwanted desorption is a significant problem.

InnovaCoat™
GOLD nanoparticles can be provided in a variety of formats (e.g. 10 OD liquid or as lyophilized nanoparticles) formulated to allow a one-step reaction with antibodies.

The kits available are designed for research use and for the development of diagnostic products, specifically for the screening of potential antibodies or small scale ‘proof of principle’ studies. Please contact us if you require any additional information and to discuss your bulk or custom conjugation requirements.

奈米金抗體標誌/注意事項/抗體純化

streptavidin金球是屬於covalent conjugates, 若要離心建議10,000rpm,5~10分。離心不會甩掉或碰掉streptavidin。

The InnovaCoat GOLD kit does not require any spinning/ washing
step, because it has been developed to provide a concentrated conjugate (20.0OD) and 100% Ab recovery. However, if your customers want to spin the gold colloid I suggest no more than 5-10 minutes at 10,000 rpm in a microfuge each time (no more than 2-3 in total).

移除抗體內BSA、Azide、Tris、glycine等物質,
必須使用Abselect的kit<可以重覆使用。【Abselect kit 抗體純化套組】資料如下連結

 

商品化產品/奈米金-Streptavidin/gold-streptavidin

【奈米金GOLD Streptavidin 】貨號#250-0200 可承受最極端的條件（2.5M的NaOH在70℃〜2小時）。以共價鍵結將streptavidin附著於奈米金，性質穩定。

【奈米金GOLD Biotin 】貨號#240-0200 可承受最極端的條件（2.5M的NaOH在70℃〜2小時）。以共價鍵結將biotin附著於奈米金，性質穩定。