【SV40-永生化毛囊真皮乳頭細胞】Immortalized Hair Follicle Dermal Papilla Cells – SV40貨號T0500
Abmgood不朽化系列商品 (immortalized cell)-Immortalized Hair Follicle Dermal Papilla Cells – SV40
人毛囊真皮乳頭細胞是從頭皮毛囊細胞外基質中分離出的頭髮乳頭分離的高活性間充質細胞。 真皮乳頭細胞通過涉及毛囊細胞的上皮 – 間質相互作用在控制毛髮生長周期和產生中起重要作用。 他們的生存受到信號轉導途徑如ERK和Akt的調節。 這些細胞可用於發育和評價毛髮生長產物並鑑定毛囊內的細胞群。 此外,它們可用於雄激素阻斷試劑的體外篩選,因為它們具有雄激素受體。
毛囊真皮乳頭細胞 – SV40 (Male) 細胞特性: Adherent / Multipolar | 不杇方法: SV40 | 來源Hair Follicle。細胞培養條件:使用PriCoat™T25培養瓶 (貨號G299),表面塗有I型膠原組成的細胞外基質,以最佳化初代和不朽細胞的生長、增殖和擴增。或塗層細胞外基質(Applied Cell Extracellular Matrix) 增強細胞貼附 (貨號G422)。Grow cells in ECM-coated culture vessels with the following conditions. 培養液TM003,胎牛血清 (TM999) 至最終濃度10% 填加1% Penicillin/Streptomycin 。1%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C。
Cat. No. | T0500 |
Name | Immortalized Hair Follicle Dermal Papilla Cells – SV40 |
Description |
Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. Their survival is regulated by signal transduction pathways such as ERK and Akt. These cells can be used for development and evaluation of hair growth products and identifying cell populations within the hair follicle. In addition, they can be used for in vitro screening of androgen blocking reagents since they have androgen receptors. |
Organism | Human (H. sapiens) |
Tissue | Hair Follicle |
Donor History | Male, 66, Caucasian |
Growth Properties | Adherent, multipolar |
Cell Type | Immortalized Cells |
Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
Shipping Conditions | Ship with dry ice. |
Product Format | Frozen |
Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
BioSafety | II |
Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
Growth Conditions | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂ |
細胞特性: 型態為Multipolar。細胞培養液更換大約1週2-3次;細胞繼代為胰蛋白酶溶液0.25% trypsin(TM050) 的下37℃下incubator 3-5分鐘,直到細胞環繞,在顯微鏡下不時監測。細胞外基質Coating dish,用PBS稀釋G422(1:1)並塗覆更少的時間。膠原蛋白含量較高,從而使與細胞的結合更強。可以嘗試減少incubator時間以及塗覆更薄的層。
HOT!! 黴漿菌PCR偵測 | DAPI染核 | 細胞內黴漿菌去除劑 | 水浴槽抑菌劑 | 噴霧式去黴劑 等系列商品
Abmgood不朽化系列商品 (immortalized reagenet) Ready-to-use
Abmgood公司於人類與老鼠不朽之細胞株上,具有幾十年的實驗經驗;提供一系列不朽 (immortalized)化之細胞株、及不朽化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus,可直接加於細胞中進行感染表現;病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液;亦提供一系列病毒力價濃縮與定量試劑商品。
細胞不朽化的工具中,SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因(p53、Rb等)的不活化相關,會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞,SV40 T anbtigen會誘導端粒酶(Telomerase)的活性。與端粒(telomere)長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中,hTERT是處於不活化態,必要時會進行活化保持telomere的長度,避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現(over-expression)對於誘發細胞不朽化是無效的(如初代上皮細胞),反而會造成細胞死亡;同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。