【LDL低密度脂蛋白檢測】 LDL細胞攝取分析 /OxLDL氧化低密度脂蛋白檢測/HDL/LDL/VLDL檢測
【LDL低密度脂蛋白檢測】 LDL攝取分析 /OxLDL氧化低密度脂蛋白檢測/測定HDL/LDL/VLDL含量-Abcam (Biovision)
低密度脂蛋白(Low-density lipoprotein , LDL)是脂蛋白 (lipoprotein)的五大主要組群之一,LDL低密度脂蛋白(Low-density lipoprotein , LDL)是一種大球形大分子,可作為“shuttle穿梭”,通過血液的水性環境將hydrophobic molecules疏水性分子(lipids脂質和cholesterol膽固醇)輸送到全身。 LDL顆粒由膽固醇酯 (cholesterol esters) 的疏水核心(與hydroxyl group羥基連接的long-chain fatty acids長鏈脂肪酸)和由含有510kDa蛋白質載脂蛋白B-100,各種磷脂 (phospholipids) 和非酯化膽固醇 (non-esterified cholesterol) 分子的兩親殼包圍的甘油三酯組成。 LDL通過特異性受體介導的內吞作用被細胞如肝細胞(hepatocytes) ,巨噬細胞(macrophages)和內皮細胞(endothelial cells)攝取。低密度脂蛋白LDL將脂肪分子傳遞給細胞,如果它們在動脈 (arteries) 壁內被氧化,就可以驅動動脈粥樣硬化 (atherosclerosis) 的進展。 由於氧化的低密度脂蛋白(Oxidized low-density lipoproteins , OxLDL)更容易被蛋白多醣 (proteoglycans) 保留,因此當它們侵入內皮 (endothelium) 時會有心血管疾病 (cardiovascular disease) 的風險。因此,高水平的LDL與動脈炎症(arterial inflammation)有關,導致心血管疾病(CVD)如動脈粥樣硬化(arterial inflammation),缺血性中風(ischemic stroke) 和心肌梗塞(myocardial infarction)。 LDL顆粒可以通過發炎的血管內皮(vascular endothelial) 緊密連接遷移,在那裡它們被氧化並吸引循環的單核細胞 (monocytes) 。單核細胞(Monocytes differentiate)分化成活化的巨噬細胞(macrophages),其進一步積累LDL並變成泡沫細胞(foam cells),導致動脈壁增厚/瘢痕形成(arterial wall thickening/scaring)和纖維性脂肪斑塊的形成(fibrous fatty plaques)。目前的CVD預防療法 (CVD preventative therapies) 包括用他汀類藥物(statins)降低LDL,其通過抑制HMG-CoA還原酶來預防內源性膽固醇 (endogenous cholesterol) 的生物合成。然而,還研究了通過增加肝臟LDL再攝取來抑制巨噬細胞攝取LDL或增強LDL清除的藥物。 BioVision的 LDL攝取分析試劑盒(細胞)K436可測量各種細胞類型的受體介導的LDL攝取含量。應用OxLDL氧化低密度脂蛋白 E4517-100 可定量測定小鼠血清,血漿和細胞培養上清液中的OxLDL。HDL高密度脂蛋白/ LDL低密度脂蛋白/非常低密度脂蛋白檢測試劑盒 | K613用以測定HDL/LDL/VLDL含量。

OxLDL (Mouse) ELISA Kit
產品描述:低密度脂蛋白(Low-density lipoprotein , LDL)是脂蛋白 ( lipoprotein) 的五大主要組群之一,其在細胞外水中將所有脂肪分子運輸到身體周圍。 LDL將脂肪分子傳遞給細胞,如果它們在動脈壁內被氧化,就可以驅動動脈粥樣硬化 (atherosclerosis) 的進展。 由於氧化的低密度脂蛋白(OxLDL)更容易被蛋白多醣保留,因此當它們侵入內皮時會有心血管疾病的風險。 BioVision的OxLDL ELISA試劑盒是一種夾心ELISA檢測方法,用於定量測定小鼠血清 (serum) ,血漿(plasma) 和細胞培養上清液中 (cell culture supernatants) 的OxLDL。 顏色密度與從樣品中捕獲的OxLDL的量成比例。

| Cat # +Size | E4517-100 |
| Size | 100 assays |
| Kit Summary | • Detection method- Absorbance (450 nm) • Species reactivity- Mouse • Application- quantitative measurement of OxLDL in Mouse serum, plasma, tissue homogenates and other biological fluids. |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Mouse |
| Applications | This ELISA kit is used for quantitative measurement of OxLDL in Mouse serum, plasma, tissue homogenates and other biological fluids. |
| Features & Benefits | • Easy, convenient and time-saving method to measure the level of OxLDL in Mouse serum, plasma, tissue homogenates, culture supernatants and other biological fluids. • Detection Range: 0.313 – 20 ng/ml • Sensitivity: < 0.188 ng/ml • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% • This assay has high sensitivity and excellent specificity for detection of OxLDL. No significant cross-reactivity or interference between OxLDL and analogues was observed. |
| Kit Components | • Micro ELISA Plate • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers |
LDL Uptake Assay Kit (Cell-Based)

產品描述:低密度脂蛋白(Low-density lipoprotein , LDL)是一種大球形大分子,可作為“shuttle穿梭”,通過血液的水性環境將hydrophobic molecules疏水性分子(lipids脂質和cholesterol膽固醇)輸送到全身。 LDL顆粒由膽固醇酯 (cholesterol esters) 的疏水核心(與hydroxyl group羥基連接的long-chain fatty acids長鏈脂肪酸)和由含有510kDa蛋白質載脂蛋白B-100,各種磷脂 (phospholipids) 和非酯化膽固醇 (non-esterified cholesterol) 分子的兩親殼包圍的甘油三酯組成。 LDL通過特異性受體介導的內吞作用被細胞如肝細胞(hepatocytes) ,巨噬細胞(macrophages)和內皮細胞(endothelial cells)攝取。高水平的LDL與動脈炎症(arterial inflammation)有關,導致心血管疾病(CVD)如動脈粥樣硬化(arterial inflammation),缺血性中風(ischemic stroke) 和心肌梗塞(myocardial infarction)。 LDL顆粒可以通過發炎的血管內皮(vascular endothelial) 緊密連接遷移,在那裡它們被氧化並吸引循環的單核細胞 (monocytes) 。單核細胞(Monocytes differentiate)分化成活化的巨噬細胞(macrophages),其進一步積累LDL並變成泡沫細胞(foam cells),導致動脈壁增厚/瘢痕形成(arterial wall thickening/scaring)和纖維性脂肪斑塊的形成(fibrous fatty plaques)。目前的CVD預防療法 (CVD preventative therapies) 包括用他汀類藥物(statins)降低LDL,其通過抑制HMG-CoA還原酶來預防內源性膽固醇 (endogenous cholesterol) 的生物合成。然而,還研究了通過增加肝臟LDL再攝取來抑制巨噬細胞攝取LDL或增強LDL清除的藥物。 BioVision的LDL攝取分析試劑盒可測量各種細胞類型的受體介導的LDL攝取。該試劑盒包含fluorescent human LDL,具明亮高度光穩定的螢光團共價標記,當被細胞攝取時可以檢測到(Ex / Em = 488 / 523nm)。未標記的human LDL包含在試劑盒中,用於驗證特異性標記的LDL攝取。該試劑盒包含一整套足以進行100次反應的試劑和96孔polylysine塗層的黑色細胞培養板,具有薄的顯微鏡級透明底部份。


| Cat # +Size | K436-100 |
| Size | 100 assays |
| Kit Summary | Easy-to-follow protocol for the measurement of LDL uptake in mammalian cell lines. |
| Detection Method | Fluorometric (Ex/Em= 488/523 nm) |
| Species Reactivity | All |
| Applications | Screening and characterization of drugs or treatment conditions that inhibit or stimulate LDL uptake,Characterization of LDL uptake in different cell types. |
| Features & Benefits | • Measure LDL uptake in different cell types • Non-Radioactive • Contains fluorescent human LDL • which is covalently-labeled with a bright • highly photostable fluorophore • Screen • study or characterize stimulators or inhibitors of LDL uptake |
| Kit Components | • Flurophore-Labeled LDL (5 mg/ml) • Unlabeled LDL • Polylysine-Coated Cell Culture Plate • Uptake Assay Buffer |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
HDL高密度脂蛋白/ LDL低密度脂蛋白/非常低密度脂蛋白檢測試劑盒 | K613-100
Acetoacetate Colorimetric Assay Kit

HDL(高密度脂蛋白) – 膽固醇和LDL(低密度脂蛋白) – 膽固醇的調節在各種疾病發展中扮演核心作用。 眾所周知,低水平的HDL和高水平的LDL與心血管事件風險增加有關。 BioVision的HDL和LDL / VLDL膽固醇定量試劑盒在血清樣品中將LDL和VLDL(極低密度脂蛋白)方便分離後,提供HDL和LDL / VLDL的簡單定量方法。 在測定中,膽固醇氧化酶特異性識別游離膽固醇並產生與探針反應以產生顏色(570nm)和螢光(Ex / Em = 538/587nm)的產物。 膽固醇酯酶將膽固醇酯水解成游離膽固醇,因此膽固醇酯和游離膽固醇可以在反應中膽固醇酯酶存在和不存在的情況下分別檢測。
| Cat # +Size | K613-100 |
| Size | 100 assays |
| Detection Method | Fluorescence Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) |
| Species Reactivity | Mammalian |
| Applications | N/A |
| Features & Benefits | • Simple procedure; takes ~1 hour • Fast and convenient |
| Kit Components | • Cholesterol Assay Buffer • 2X LDL/VLDL Precipitation Buffer • Cholesterol Probe (in DMSO, anhydrous) • Enzyme Mix (Lyophilized) • Cholesterol Esterase (Lyophilized) • Cholesterol Standard (2 µg/µl) |
| Cat # +Size | K597-100 |
| Size | 100 assays |
| Detection Method | Absorbance (OD 450 nm). |
| Applications | Measurement of Succinyl-CoA Synthetase activity in various tissues/cells Analysis of cell signaling pathway |
| Features & Benefits | • Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples. |
| Kit Components | • SCS Assay Buffer • SCS Substrate Mix (Lyophilized) • SCS Enzyme Mix (Lyophilized) • SCS Developer (Lyophilized) • NADH Standard (Lyophilized) • SCS Positive Control (Lyophilized) |
低密度脂蛋白(LDL)檢測試劑盒 (酵素免疫吸附試驗法) | 貨號SEB107Mu
ELISA Kit for Low Density Lipoprotein (LDL)
商品: 低密度脂蛋白(LDL)檢測試劑盒 (酶聯免疫吸附試驗法) Double-antibody Sandwich貨號SEB107Mu,應用物種Mus musculus (Mouse) ;偵測範圍6.25-400ng/mL, 最低靈敏度2.54ng/mL可應用於Serum, plasma and other biological fluids生物樣品之檢測。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。
低密度脂蛋白(LDL)檢測試劑盒套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
LDL攝取分析試劑盒(細胞) | OxLDL 氧化低密度脂蛋白檢測 | HDL高密度脂蛋白/ LDL低密度脂蛋白/非常低密度脂蛋白檢測試劑盒
低密度脂蛋白(LDL)檢測相關之抗體 | 蛋白質 | ELISA | CLIA產品
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEB107Mu | ELISA Kit for Low Density Lipoprotein (LDL) | Enzyme-linked immunosorbent assay for Antigen Detection. |